A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
The presence of P+PBac{XP5.RB3}BSC890 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The breakpoints of Df(2R)BSC890 predicted from the Release 5 genomic coordinates of the progenitor P{XP}CG11163d05998 and PBac{RB}CG30431e01618 transposable element insertion sites are 2R: 1669744--1669934;2090851 and the cytological breakpoints predicted from these coordinates are 41F11;42A13.