Isolation and characterization of Df(2R)BSC890
Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC890 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG11163d05998 and PBac{RB}CG30431e01618. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG11163d05998/PBac{RB}CG30431e01618 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC890 from the segment of P{XP}CG11163d05998 to the left of its FRT site and the segment of PBac{RB}CG30431e01618 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods. The breakpoints of Df(2R)BSC890 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 2R: 1669744--1669934;2090851 and the cytological breakpoints predicted from these coordinates are 41F11;42A13. Df(2R)BSC890 failed to complement EcRM544fs.
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Kevin Cook, Ph.D.
Bloomington Drosophila Stock Center
Department of Biology
Indiana University
1001 E. Third St.
Bloomington, IN 47405-7005
