actin, F-actin, BAP47, C, Act42
Gene model reviewed during 5.47
There is only one protein coding transcript and one polypeptide associated with this gene
Oxidation of Met-45 by Mical to form methionine sulfoxide promotes actin filament depolymerization. Methionine sulfoxide is produced stereospecifically, but it is not known whether the (S)-S-oxide or the (R)-S-oxide is produced.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Act42A using the Feature Mapper tool.
Act42A transcripts are most abundant in embryos. They are evenly distributed in preblastoderm embryos, becoming localized to the periphery at blastoderm formation. Later they are observed in the brain and ventral nerve cord as well as in the developing gonad, proventriculus, and midgut.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Act42A in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
Age related changes in Act42A expression have been studied by Northern analysis.
Sequence analysis demonstrates insect muscle actins form a distinct family of closely related proteins significantly diverged from cytoplasmic actins.
Translation of actin RNA in early embryos injected with initiation factors has been studied.
The expression of HIS-C genes during oogenesis has been studied, and compared to periods of DNA synthesis and actin expression during this developmental stage.
Codes for cytoplasmic actin; transcribed throughout development; one of two actin genes transcribed in Kc cells and several other cell lines (FBrf0038990). One of three actin genes expressed in the wing disc during wing development, each with its characteristic profile. Peak expression at 44h of pupal age, little or no expression at 60h rising again at 80h. 44h peak corresponds to time of maximum change in cell shape (FBrf0042391). Transcription increased 6-8 fold by 20-OH-ecdysone in tissue culture cells (FBrf0047102; FBrf0064343).