FB2026_03 , released September 17, 2026
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Term edman degradation ID (Ontology) MI:0039 (Molecular Interactions)
Definition In this procedure the N-terminus amino acid is cleaved from a polypeptide and identified by high-pressure liquid chromatography. The cycle is repeated on the ever-shortening polypeptide until all the residues are identified. On average only 20-30 consecutive cycles can be performed and lead to amino acid identification. Longer polypeptides or full length proteins must be cleaved by specific protease before Edman degradation and their sequences built by fragment overlapping.[ PubMed:14755292 ]
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  protein sequence identification
   |__partial identification of protein sequence
       |__edman degradation  13 rec.
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