FlyBase Gene Report: Dmel\Fhos

General Information

Symbol

Dmel\Fhos

Species

D. melanogaster

Name

Formin homology 2 domain containing

Annotation Symbol

CG42610

Feature Type

protein_coding_gene

FlyBase ID

FBgn0266084

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Formin homology 2 domain containing (Fhos) encodes a protein that regulates actin-dependent remodeling of muscle cytoskeleton via induction of actin stress fibers, directs macrophage migration during cellular immune response, and is involved in autophagic programmed cell death. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

l(3)01629, desert, knittrig

Key Links

Genomic Location

Cytogenetic map

66D12-66D15

Sequence location

3L:8,753,619..8,798,719 [+]

Recombination map

3-26

RefSeq locus

NT_037436 REGION:8753619..8798719

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN002509352

(GO Reference Genome Project, 2011-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in imaginal disc-derived wing morphogenesis

inferred from mutant phenotype

(Lammel et al., 2014)

involved_in muscle cell cellular homeostasis

inferred from mutant phenotype

(Kucherenko et al., 2011)

inferred from genetic interaction with FLYBASE:Dg; FB:FBgn0034072

(Kucherenko et al., 2011)

inferred from genetic interaction with FLYBASE:Dys; FB:FBgn0260003

(Kucherenko et al., 2011)

involved_in muscle system process

inferred from expression pattern

(Montana and Littleton, 2006)

inferred from genetic interaction with FLYBASE:Mhc; FB:FBgn0264695

(Montana and Littleton, 2006)

involved_in positive regulation of cell migration

inferred from mutant phenotype

(Lammel et al., 2014)

involved_in positive regulation of stress fiber assembly

inferred from mutant phenotype

(Lammel et al., 2014)

inferred from genetic interaction with FLYBASE:Rok; FB:FBgn0026181

(Lammel et al., 2014)

involved_in positive regulation of substrate adhesion-dependent cell spreading

inferred from mutant phenotype

(Lammel et al., 2014)

involved_in programmed cell death involved in cell development

inferred from mutant phenotype

(Anhezini et al., 2012)

involved_in salivary gland histolysis

inferred from mutant phenotype

(Anhezini et al., 2012)

involved_in trachea morphogenesis

inferred from mutant phenotype

(Lammel et al., 2014)

involved_in wound healing, spreading of cells

inferred from mutant phenotype

(Lammel et al., 2014)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from biological aspect of ancestor with PANTHER:PTN008701008

(GO Reference Genome Project, 2011-)

involved_in positive regulation of stress fiber assembly

inferred from biological aspect of ancestor with PANTHER:PTN001143929

(GO Reference Genome Project, 2011-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Lammel et al., 2014)

located_in nucleus

inferred from direct assay

(Anhezini et al., 2012)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Lammel et al., 2014, Anhezini et al., 2012)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002509352

(GO Reference Genome Project, 2011-)

is_active_in cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN002509352

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Fhos for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry E1JI78)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Fhos-RA

FBtr0302085

NM_168293

5027

1393

Fhos-RB

FBtr0302086

NM_168294

4347

1294

Fhos-RD

FBtr0302583

NM_001202143

5622

1509

Fhos-RE

FBtr0302584

NM_001202142

5159

1387

Fhos-RF

FBtr0306630

NM_001259753

4838

1205

Fhos-RG

FBtr0306631

NM_001259754

4304

1152

Fhos-RH

FBtr0306632

NM_001259755

6029

1744

Fhos-RI

FBtr0333888

NM_001274670

8968

2528

Fhos-RJ

FBtr0333889

NM_001169916

8444

2528

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Fhos-PA

FBpp0291295

155.9

1393

7.39

FBpp0291296

144.5

1294

6.90

FBpp0291739

169.6

1509

8.63

FBpp0291740

155.1

1387

7.62

FBpp0297585

135.0

1205

8.32

FBpp0297586

129.7

1152

7.20

FBpp0297587

196.0

1744

5.86

FBpp0306020

279.2

2528

8.18

FBpp0306021

279.2

2528

8.18

E1JI78

NP_001163387

ACZ94658

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2528 aa isoforms: Fhos-PI, Fhos-PJ

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fhos using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.51

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 9 -- 12

midline primordium | subset

(Kearney et al., 2004)

embryonic stage 12

embryonic/larval salivary gland

(Lammel et al., 2014)

midline glial cell

(Watson et al., 2011)

embryonic stage 12 -- 17

midline glial cell

(Lammel et al., 2014)

embryonic stage 13 -- 16

embryonic/larval salivary gland body

(Fisher et al., 2012)

ventral midline of embryo

(Fisher et al., 2012)

larval tracheal system

(Lammel et al., 2014)

midline glial cell

(Kearney et al., 2004)

embryonic stage 15 -- 17

muscle cell | subset

(Tanaka et al., 2004)

larval ventral nerve cord | restricted

(Tanaka et al., 2004)

ventral midline of embryo

(Tanaka et al., 2004)

embryonic stage 16

cercus

Comment: surrounding

(Lammel et al., 2014)

embryonic/larval hemocyte

(Lammel et al., 2014)

embryonic/larval dorsal vessel

(Lammel et al., 2014)

tracheal tip cell

(Lammel et al., 2014)

larval stage

midline glial cell

(Lammel et al., 2014)

third instar larval stage

imaginal disc

(Lammel et al., 2014)

eye disc

(Lammel et al., 2014)

wing disc

(Lammel et al., 2014)

eye disc morphogenetic furrow

(Lammel et al., 2014)

wing margin

(Lammel et al., 2014)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P1 -- pupal stage P5

organism

(Anhezini et al., 2012)

adult stage

organism

(Anhezini et al., 2012)

Additional Descriptive Data

Fhos is expressed in anterior midline glia but not in posterior midline glia in stage 12 embryos.

(Watson et al., 2011)

Expression pattern inferred from unspecified enhancer trap line.

(Kearney et al., 2004)

Expression of Fhos is observed in a subset of muscles, and at the midline of the ventral nerve cord from stages 15-17.

(Tanaka et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P4 -- pupal stage P5

embryonic/larval salivary gland

(Anhezini et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Fhos protein moves from cytoplasm to nucleus as cell death begins in the salivary gland, around 11h APF, which is at the end of stage P4(i).

(Anhezini et al., 2012)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Lammel et al., 2014)

located_in nucleus

inferred from direct assay

(Anhezini et al., 2012)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Lammel et al., 2014, Anhezini et al., 2012)

Expression Deduced from Reporters

Reporter: P{lwB}AA142

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

midline glial cell

(Klambt et al., 1991)

embryonic stage 12

embryonic/larval salivary gland

(Lammel et al., 2014)

embryonic stage 12 -- 17

midline glial cell

(Lammel et al., 2014)

embryonic stage 13 -- 16

larval tracheal system

(Lammel et al., 2014)

embryonic stage 15

midline glial cell

(Klaes et al., 1994)

embryonic stage 16

midline glial cell

(Ito et al., 1995, Jones et al., 1995)

cercus

Comment: surrounding

(Lammel et al., 2014)

embryonic/larval hemocyte

(Lammel et al., 2014)

embryonic/larval dorsal vessel

(Lammel et al., 2014)

tracheal tip cell

(Lammel et al., 2014)

larval stage

midline glial cell

(Lammel et al., 2014)

first instar larval stage

glial cell of larval central nervous system

(Ito et al., 1995)

larval central nervous system | restricted

(Ito et al., 1995)

third instar larval stage

imaginal disc

(Lammel et al., 2014)

eye disc

(Lammel et al., 2014)

wing disc

(Lammel et al., 2014)

eye disc morphogenetic furrow

(Lammel et al., 2014)

wing margin

(Lammel et al., 2014)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fhos in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Fhos

View exonic expression by tissue for Dmel\Fhos

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266084

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39004/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266084

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266084

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32030

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 36 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Fhos

Transgenic constructs containing regulatory region of Fhos

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

Df(3L)ED4413

(Lammel et al., 2014)

Df(3L)ED4421

(Lammel et al., 2014)

Df(3L)hry-i22

(Lammel et al., 2014, Desbordes et al., 2005, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(3L)Scf-R6

(Lammel et al., 2014, Desbordes et al., 2005)

Gene partially disrupted in

Df(3L)knittrigΔ1

(Lammel et al., 2014)

Df(3L)knittrigΔ140

(Lammel et al., 2014)

Gene not disrupted in

Df(3L)BSC816

(Lammel et al., 2014)

Df(3L)ED4416

(Lammel et al., 2014)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Fhos⁰¹⁶²⁹

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Fhos^GD2374

partially lethal - majority die (with Df(3L)knittrigΔ1)

partially lethal - majority live (with Df(3L)knittrigΔ1)

Fhos^MI04231

viable

Fhos^EY09842

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

Fhos^GD10435

viable, with Scer\GAL4^pnr-MD237

Fhos^GD10435

viable, with Scer\GAL4^tin.CΔ4

Fhos^GD10435

Sterility

Allele

fertile

Fhos^EY09842

Other Phenotypes

Allele

abnormal cell death

abnormal cell death (with Df(3L)hry-i22)

Fhos⁰¹⁶²⁹

abnormal cell shape, with Scer\GAL4^Hml.Δ

Fhos^GD10435

abnormal cell shape | third instar larval stage, with Scer\GAL4^ptc.PU

abnormal locomotor behavior | dominant | temperature conditional

Fhos^A055

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Fhos^GD2374

abnormal stress response

Fhos^BG01659

abnormal wound healing, with Scer\GAL4^Hml.Δ

Fhos^GD10435

decreased size | adult stage, with Scer\GAL4^Mef2.PU

Fhos^KK108388

flightless, with Scer\GAL4^Mef2.PR

Fhos^GD2374

some die during pupal stage, with Scer\GAL4^pnr-MD237

Fhos^GD2374

Phenotype manifest in

Allele

actin filament bundle, with Scer\GAL4^unspecified

embryonic/larval ganglionic tracheal branch (with Df(3L)knittrigΔ1)

embryonic/larval plasmatocyte, with Scer\GAL4^Hml.Δ

Fhos^GD10435

embryonic/larval plasmatocyte | third instar larval stage, with Scer\GAL4^Hml.Δ

Fhos^{ΔDAD.UAS.EGFP}

embryonic/larval salivary gland, with Scer\GAL4^unspecified

embryonic/larval salivary gland | pupal stage

embryonic/larval salivary gland | pupal stage (with Df(3L)hry-i22)

Fhos⁰¹⁶²⁹

indirect flight muscle | adult stage, with Scer\GAL4^Mef2.PU

Fhos^KK108388

indirect flight muscle cell

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

Fhos^GD2374

indirect flight muscle cell (with Fhos^A055)

Fhos^EY09842

indirect flight muscle cell (with Fhos^EY09842)

Fhos^A055

myofibril, with Scer\GAL4^Mef2.PR

Fhos^GD2374

sarcomere, with Scer\GAL4^Mef2.PR

Fhos^GD2374

sarcomere | adult stage, with Scer\GAL4^Mef2.PU

Fhos^KK108388

stress fiber | third instar larval stage, with Scer\GAL4^Hml.Δ

Fhos^{ΔDAD.UAS.EGFP}

wing (with Df(3L)knittrigΔ1)

Fhos^EMS

wing (with Df(3L)knittrigΔ140)

Fhos^EMS

wing disc | third instar larval stage, with Scer\GAL4^ptc.PU

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (15)

Hsap\FHOD1

10 of 14

Yes

Hsap\FHOD3

10 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Fhod3

10 of 14

Yes

Rnor\Fhod1

8 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (14)

Mmus\Fhod3

10 of 14

Yes

Mmus\Fhod1

9 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\fhod1

6 of 13

Yes

Xtro\fhod3

6 of 13

Yes

Xtro\arhgef25

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\slc1a7

1 of 13

Danio rerio (Zebrafish) (23)

Drer\fhod3a

9 of 14

Yes

Drer\fhod1

8 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\fhod-1

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP004873

10 of 12

Yes

Agam\AgaP_AGAP004874

2 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (25)

Atha\AFH1

2 of 13

Yes

Atha\AT2G43800

2 of 13

Yes

Atha\AT3G05470

2 of 13

Yes

Atha\AT3G07540

2 of 13

Yes

Atha\AT5G07740

2 of 13

Yes

Atha\AT5G48360

2 of 13

Yes

Atha\FH6

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\BNI1

1 of 13

Yes

Scer\BNR1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Fhos. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

dia

2 of 13

capu

1 of 13

DAAM

1 of 13

form3

1 of 13

Frl

1 of 13

mwh

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Fhos^EY09842

exacerbates muscular dystrophy

modeled by Dg^O86

(Kucherenko et al., 2011)

modeled by Dg^RNAi.UAS

(Kucherenko et al., 2011)

Fhos^A055

exacerbates muscular dystrophy

modeled by Dg³²³

(Kucherenko et al., 2011)

modeled by Dg^RNAi.UAS

(Kucherenko et al., 2011)

Fhos^HMJ21037

ameliorates synucleinopathy

modeled by Hsap\SNCA^QUAS.cOa

(Ordonez et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

FHOD1; formin homology 2 domain containing 1

10 of 14

606881

FHOD3; formin homology 2 domain containing 3

10 of 14

609691

CARDIOMYOPATHY, FAMILIAL HYPERTROPHIC, 28; CMH28

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Fhos - Rac1

pull down, anti tag western blot

(Lammel et al., 2014)

Fhos - Rho1

pull down, anti tag western blot

(Lammel et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Fhos

(Kucherenko et al., 2011)

sals

enhanceable

Fhos

(Farkas et al., 2024)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0266084

MIST (protein-protein) - An integrated Molecular Interaction Database

39004

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-26

Cytogenetic map

66D12-66D15

Sequence location

3L:8,753,619..8,798,719 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66D12-66D15

Limits computationally determined from genome sequence between P{PZ}SrpRβ^rK561&P{lacW}l(3)j5B6^j5B6 and P{PZ}l(3)01629⁰¹⁶²⁹&P{PZ}mRpL12¹⁰⁵³⁴; Limits computationally determined from genome sequence between P{PZ}l(3)01629⁰¹⁶²⁹&P{PZ}mRpL12¹⁰⁵³⁴ and P{EP}Hsp26^EP3336&P{EP}Hsp26^EP3315

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66D-66E

(Erkner, 1999.12.20)

66E1-66E2

(determined by in situ hybridisation)

(Spradling et al., 1999)

66E1-66E2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-26

(FlyBase, 2016)

3-20.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31400

y¹ v¹; P{TRiP.JF01606}attP2

51391

y¹ v¹; P{TRiP.HMJ21037}attP40

17612

y¹ w^67c23; P{EPgy2}Fhos^EY09842

14014

y¹ w^67c23; P{SUPor-P}Fhos^KG05956 ry⁵⁰⁶

11540

P{PZ}Fhos⁰¹⁶²⁹ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

Kyoto

103977

y^* w^*; P{GawB}Fhos^NP1308 / TM6, P{UAS-lacZ.UW23-1}UW23-1

105036

y^* w^*; P{GawB}Fhos^NP5482 / TM6, P{UAS-lacZ.UW23-1}UW23-1

NIG-Fly

5797R-2

P{NIG.5797R}2

HMJ21037

y¹ v¹; P{TRiP.HMJ21037}attP40/CyO

VDRC

v108347

P{KK108388}VIE-260B

v34034

w¹¹¹⁸; P{GD10435}v34034

v318116

PBac{fTRG00478.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (29)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (40)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD24110

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0266084

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Shwartz et al., 2016, Anhezini et al., 2012)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

New annotation (CG32025) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: Fhos desert

(FlyBase Genome Annotators, 2009)

Source for merge of: CG5775 CG5797

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: Fhos l(3)01629

(Anhezini et al., 2012)

Additional comments

Annotations CG32025 and CG32030 merged as CG42610 in release 5.20 of the genome annotation.

(FlyBase Genome Annotators, 2009)

Annotations CG5775 and CG5797 merged as CG32030 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Nomenclature History

Source for database identify of

Source for identity of: Fhos CG32030

(Montana and Littleton, 2006)

Nomenclature comments

Etymology

Named 'knittrig' (German for 'wrinkled') based on the wing phenotype of mutant adult flies.

(Lammel et al., 2014)

Gene named 'jazigo' which stands for "grave" in Brazilian Portuguese.

(Anhezini et al., 2012)

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CG32025

CG32030

(Anhezini et al., 2012, Cammarato et al., 2011, Keleman et al., 2009.8.5, Ni et al., 2008, Bakal et al., 2007, Firth and Baker, 2007, Valanne et al., 2007, Zhang et al., 2007, Girard et al., 2006, Johansson et al., 2005, Kim et al., 2005, Lipatov et al., 2005)

CG42610

(Ni et al., 2010.12.1, FlyBase Genome Annotators, 2009)

CG5775

(Kurshakova et al., 2019, Lai et al., 2007, Montana and Littleton, 2006)

CG5797

(Montana and Littleton, 2006, Huntley and Golding, 2004, Tanaka et al., 2004, Boutros et al., 2002, Goldstein and Gunawardena, 2000)

dFHOS

(Montana and Littleton, 2006)

desert

(Zhang et al., 2007)

fhos

(Bischoff et al., 2021)

jaz

(Anhezini et al., 2012)

knit

(Lammel et al., 2014)

l(3)01629

(Anhezini et al., 2012, McClure and Schubiger, 2008)

Name Synonyms

CG32030

Fhos

(Chakraborty et al., 2018)

Formin homology 2 domain containing

Formin homology 2 domain containing ortholog

desert

jazigo

(Anhezini et al., 2012)

knittrig

(Pinheiro et al., 2017, Lammel et al., 2014)

lethal (3) 01629

Secondary FlyBase IDs

FBgn0261259
FBgn0052030
FBgn0035925
FBgn0035927
FBgn0040229
FBgn0052025
FBgn0010755

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 103 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39004

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

E1JI78

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

E1JI78

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0266084

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39004/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0266084

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0266084

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG42610

MARRVEL_MODEL - MARRVEL (model organism gene)

39004

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0266084

DroID - A comprehensive database of gene and protein interactions.

FBgn0266084

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0266084

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32030

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032667

MIST (protein-protein) - An integrated Molecular Interaction Database

39004

References (113)

The following external sites may use different assemblies or annotations than FlyBase.

-0.51

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