FlyBase Allele Report: Dmel\Myo61F[RNAi.UAS]

General Information

Symbol

Dmel\Myo61F^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0195156

Feature type

allele

Associated gene

Dmel\Myo61F

Associated Insertion(s)

Carried in Construct

P{UAS-Myo61F.RNAi}

Key Links

Transgenic product class

RNAi_reagent

(Hozumi et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Hozumi et al., 2006)

Mutagen

in vitro construct

(Hozumi et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-Myo61F.RNAi}

Cytology

Description

UASt regulatory sequences drive expression of Myo61F cDNA sequences (nucleotides 446-848 of GH04201) in an inverted repeat, with an interruption of the Myo61F fourth intron.

(Hozumi et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Myo61F

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval midgut primordium, with Scer\GAL4^nanos.PG

(Hozumi et al., 2006)

Detailed Description

Statement

Reference

Flies expressing Myo61F^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Myo31DF-NP1548} in the presence of Df(3L)920 show normal rotation of the male genitalia.

(Petzoldt et al., 2012)

Expression of Myo61F^{dsRNA.Scer\UAS} under the control of Scer\GAL4^nos.PG results in a defect in the left-right handedness of the midgut in 7.7% of embryos.

(Hozumi et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Myo61F^RNAi.UAS, Scer\GAL4^NP1548 is a suppressor of male genitalia phenotype of Scer\GAL4^NP1548, shg^KK103334

(Petzoldt et al., 2012)

Other

Statement

Reference

Myo31DF^RNAi.Sym.UAS, Myo61F^RNAi.UAS, Scer\GAL4^NP1548 has male genitalia phenotype

(Petzoldt et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Myo31DF^{dsRNA.Sym.Scer\UAS} and Myo61F^{dsRNA.Scer\UAS} under the control of Scer\GAL4^{Myo31DF-NP1548} (using a temperature sensitive Scer\GAL80 allele to limit expression to day 4 of development onwards) results in partial sinistral rotation of the male genitalia (instead of the wild-type dextral rotation).

Co-expression of Myo61F^{dsRNA.Scer\UAS} shifts the male genitalia rotation defects seen in flies expressing shg^KK103334 under the control of Scer\GAL4^{Myo31DF-NP1548} towards a more wild-type phenotype, with almost half of males having partial genital rotation of between 91 and 180[o].

(Petzoldt et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Used to study the phenotypic consequences of dsRNA interference (RNAi) of the Myo61F gene.

(Hozumi et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Myo61F^RNAi.UAS

Myo61F^{dsRNA.Scer\UAS}

Myo61F^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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