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General Information
Symbol
Dmel\shgKK103334
Species
D. melanogaster
Name
FlyBase ID
FBal0232287
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an inverted repeat.

Allele components
Product class / Tool use(s)
Encoded product / tool
Associated Sequence Features
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In contrast to control clusters, which migrate along a straight path and each the oocyte in ~4hr, nurse cells expressing shgKK103334 under the control of Scer\GAL4otu.T:Hsim\VP16, Scer\GAL4nos.PG and Scer\GAL4nos.UTR.T:Hsim\VP16 causes border cells to wander. A total of 70% of the clusters migrate around the outside of the egg chamber, and even those that migrate between nurse cells do not sustain directed, posterior movement.

Flies expressing shgKK103334 under the control of Scer\GAL4slbo.2.6 show differences in the migration behaviour of border cell clusters. First, a distinct front-back polarity is evident in wild-type clusters, which show spatially segregated protrusion and retraction with high protrusion velocities predominating at the front and high retraction velocities at the back. This spatial segregation is lost in clusters lacking guidance-receptor activities. Second, these double mutant clusters display overall slower protrusion and retraction velocities.

Expression of shgKK103334 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 accelerates age-dependent germline stem cell loss in females.

Expression of shgKK103334 under the control of Scer\GAL4Abd-B-LDN results in defects in male genital rotation, with males showing either no rotation, or close to no rotation (1-90[o]) of the genitalia.

Expression of shgKK103334 under the control of Scer\GAL4Myo31DF-NP1548 results in defects in male genital rotation, with male pharate adults showing either no rotation, or close to no rotation (1-90[o]) of the genitalia.

Depletion of shg in wing margin cells through expression of shgKK103334 under the control of Scer\GAL4wg-IS650 and Scer\GAL80ts.αTub84B results in severe disruption of cell organisation, with the wing margin cell region developing to be broader and necrotic and a wing margin structure that is flattened in the transverse section. Accordingly, the dorsal and ventral wing edges do not attach. In the adult wing with shg depleted from the wing margin cells, the alignment and growth of wing margin hairs is severely disrupted, and the ectopic tanning and shrinkage of the wing blade is found, probably due to necrotic disruption of the wing margin epithelial structure.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of shgKK103334 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 suppresses the retention of N55e11 mutant germline stem cells seen in females at two weeks after clone induction.

Co-expression of Myo61FdsRNA.Scer\UAS shifts the male genitalia rotation defects seen in flies expressing shgKK103334 under the control of Scer\GAL4Myo31DF-NP1548 towards a more wild-type phenotype, with almost half of males having partial genital rotation of between 91 and 180[o].

Males co-expressing shgKK103334 and α-CatGD8808 under the control of Scer\GAL4Abd-B-LDN have defects in genital rotation, either showing partial (77%) or no (23%) rotation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
shgKK103334
Name Synonyms
Secondary FlyBase IDs
    References (9)