FlyBase Allele Report: Dmel\shg[GD14421]

General Information

Symbol

Dmel\shg^GD14421

Species

D. melanogaster

Name

FlyBase ID

FBal0210573

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{GD14421}

Also Known As

UAS-shg-RNAi, UAS-shg RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14421}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14421

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Cai et al., 2014)

embryonic/larval epidermis | third instar larval stage, with Scer\GAL4^e22c

(Wang et al., 2015)

flight muscle cell, with Scer\GAL4^Mef2.PR

(Langer et al., 2010)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

myofibril, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression of shg^GD14421 under the control of Scer\GAL4^e22c results in abnormal large syncytia formation in the epidermis of third instar larvae, although the penetrance is low (less than 20% larvae).

(Wang et al., 2015)

Knockdown of shg in the outer, migratory border cells, through expression of shg^GD14421, under the control of Scer\GAL4^slbo.2.6, in contrast to wild-type, results in a significant reduction of direction persistence in migration, with clusters ending up in ectopic locations, and the migration paths wandering, rather than migrating in a straight line. Though these cells are still motile and remain clustered, shg knockdown reduces the average speed from 0.67 υm/min to 0.26 υm/min.

(Cai et al., 2014)

Animals expressing shg^GD14421 under the control of Scer\GAL4^Mef2.PR have missing flight muscles.

(Langer et al., 2010)

Adults expressing shg^GD14421 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or early pupal lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in missing adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in degenerate myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14421

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The phenotype of missing flight muscles that is seen in animals expressing shg^GD14421 under the control of Scer\GAL4^Mef2.PR is not rescued if they are carrying the P{FlyFos-045685} construct (which contains D. pseudoobscura pseudoobscura fosmid sequence that includes the region orthologous to shg), indicating either that the P{GD14421} RNAi phenotype is unspecific, or that the D. pseudoobscura shg ortholog is also targeted by the P{GD14421} hairpin.

(Langer et al., 2010)

Complementation and Rescue Data

Rescued by

shg^GD14421 is rescued by shg^{tension.Ubi-p63E}/Scer\GAL4^slbo.2.6

(Cai et al., 2014)

shg^GD14421 is rescued by Scer\GAL4^{VP16.nanos.UTR}/shg^{tension.Ubi-p63E}

(Cai et al., 2014)

shg^GD14421 is rescued by shg^tension.UAS/Scer\GAL4^slbo.2.6

(Cai et al., 2014)

shg^GD14421 is rescued by Scer\GAL4^{VP16.nanos.UTR}/shg^tension.UAS

(Cai et al., 2014)

shg^GD14421 is rescued by shg^{tension.control.Ubi-p63E}/Scer\GAL4^slbo.2.6