The expression of shg^HMS00693 under the control of Scer\GAL4^slbo.2.6 leads to border cell migration failure or delay in all egg chambers, despite of no cluster integrity defects.

The expression of shg^HMS00693 under the control of Scer\GAL4^upd1.PU leads to frequent dissociation of the border cell cluster.

Clonal expression of shg^HMS00693 (under the control of Scer\GAL4^Act.PU) in male pupal A8a epithelia cells induces an increase junction growth rate if expressed in one of the cells forming the junction, as compared to control junctions (formed by two wild-type cells).

Knockdown of shg through expression of shg^HMS00693 in nurse cells (under the control of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) results in border cells migrating more slowly than normal and to exhibit a hyperpolarized morphology. A total of 64% of protrusions from border cell clusters migrating between nurse cells are directed toward the oocyte.

Knockdown of shg through expression of shg^HMS00693 in the germline, under the control of Scer\GAL4^slbo.2.6 results in 63% of protrusions from border cell clusters migrating between wild-type nurse cells are directed toward the oocyte. Border cell clusters do not split apart in this mutant.

Knockdown of shg specifically in polar cells, through expression of shg^HMS00693 under the control of Scer\GAL4^upd.PU results in 73% of border cell clusters splitting apart.