myosin-IB, Myo1B, myosin IB, DroMIB, Myo1C
motor protein present within the microvillus of the gut apical brush border where it forms lateral tethers between the microvillus membrane and underlying actin filament core - maintains structural integrity of the brush border domain enterocyte - provides resistance against oral infection by bacterial pathogens
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.45
Gene model reviewed during 5.55
3.5 (northern blot)
1026 (aa); 118 (kD predicted)
The myosin motor domain contains the derminants for the direction of left-right body asymmetry.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Myo61F using the Feature Mapper tool.
At embryonic stage 16, Myo61F protein is concentrated at the basolateral domain of immature enterocytes and is diffusely located in the cytoplasm. At embryonic stage 17, intense apical staining is observed in most regions of the gut. In larvae and adults, Myo61F protein is found in the brush border predominantly in the apical microvillar domain. In egg chambers, it is found in the basolateral domain of follicle cells until stage 8 after which it is more apically positioned. In stage 10, the localization of Myo61F protein to the api al domain of columnar follicle cells near the oocyte is thought to correspond to the microvilli of the brush border which exists there.
Myo61F protein is localized to the brush border of enterocytes through the entire length of the larval midgut. It is also localized to the brush borders of specialized secretory cells of the digestive sytem, including the proventriculus, gastric ceaca, cuprophilic cells, and in the apical domain of epithelial cells of Malphigian tubules. In adults, Myo61F is expressed in high levels in the inner chiasm of the optic lobe, with significant expression in the lamina and medulla. Myo61F is also expressed in photoreceptors, where it is concentrated at the base of microvilli.
Myo61F protein is first detected on western blots in 12-16hr embryos, increases dramatically at the end of embryogenesis and in first instar larvae, increases slightly in later larval stages, is present at low levels in pupae and is observed at higher levels in adults. Myo61F protein is detected by immunolocalization in the alimentary canal and its derivatives and in egg chambers. In stage 17 embryos, it is observed in the gastric caeca but not in the foregut. A gradient of expression is observed in the midgut with most of the staining in the posterior midgut and increasingly less in the middle midgut and anterior midgut. Some protein is present in the hindgut. In egg chambers, protein is present in follicle cells. By stage 10, it is associated only with columnar follicle cells surrounding the oocyte. Additionally, it is associated with centripetally migrating follicle cells over the anterior of the oocyte.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Myo61F in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Myo61F has a role in maintaining the structural integrity of the brush border domain the larval midgut enterocyte and in providing resistance against oral infection by bacterial pathogens.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.