FlyBase Gene Report: Dmel\Mhcl

General Information

Symbol

Dmel\Mhcl

Species

D. melanogaster

Name

Myosin heavy chain-like

Annotation Symbol

CG31045

Feature Type

protein_coding_gene

FlyBase ID

FBgn0026059

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Myosin heavy chain-like (Mhcl) encodes a protein that interacts with the product of rols during myoblast fusion. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

PDZ-myosin

Key Links

Genomic Location

Cytogenetic map

89B2-89B3

Sequence location

3R:16,068,523..16,098,761 [-]

Recombination map

3-58

RefSeq locus

NT_033777 REGION:16068523..16098761

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables actin filament binding

inferred from direct assay

(Guzik-Lendrum et al., 2011)

NOT enables ATP binding

inferred from direct assay

(Guzik-Lendrum et al., 2011)

NOT enables ATP hydrolysis activity

inferred from direct assay

(Guzik-Lendrum et al., 2011)

NOT enables cytoskeletal motor activity

inferred from direct assay

(Guzik-Lendrum et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables actin filament binding

inferred from biological aspect of ancestor with PANTHER:PTN005210253

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in sarcomere

inferred from direct assay

(Bonn et al., 2013)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN005210253

(GO Reference Genome Project, 2011-)

part_of myosin complex

inferred from electronic annotation with InterPro:IPR002928

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001609, InterPro:IPR002928

(InterPro Project Members, 2004-)

is_active_in myosin filament

inferred from biological aspect of ancestor with PANTHER:PTN005210253

(GO Reference Genome Project, 2011-)

part_of myosin II complex

inferred from biological aspect of ancestor with PANTHER:PTN005210253

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NON-MOTOR MYOSINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Myosin heavy chain-like (Mhcl) encodes a protein that interacts with the product of rols during myoblast fusion. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

NON-MOTOR MYOSINS -: Non-motor myosins include class XX and XVIIIa myosins, which belong to the myosin superfamily but lack ATP-binding/ATPase activity and do not function as molecular motors. They are able to bind actin and are though to act as scaffold or actin tethering proteins. (Adapted from FBrf0213876 and FBrf0223842.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mhcl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q8INC3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mhcl-RA

FBtr0083229

NM_169700

6686

2148

Mhcl-RB

FBtr0083231

NM_169701

7418

2194

Mhcl-RC

FBtr0083232

NM_170650

2923

920

Mhcl-RD

FBtr0083233

NM_169703

4004

1134

Mhcl-RF

FBtr0110973

NM_001043251

6509

1923

Mhcl-RG

FBtr0110974

NM_001043250

6782

2160

Mhcl-RH

FBtr0303845

NM_001202304

2015

533

Mhcl-RI

FBtr0332439

NM_001275694

3941

1113

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mhcl-PA

FBpp0082683

240.1

2148

6.60

FBpp0082685

244.8

2194

6.70

FBpp0082686

106.7

920

5.19

FBpp0082687

128.7

1134

5.26

FBpp0110273

215.6

1923

7.04

FBpp0110274

241.6

2160

6.62

FBpp0292853

62.0

533

4.92

FBpp0304712

126.4

1113

5.29

A0A0B4KGR6

NP_001262623

AGB96003

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mhcl using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.85

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

amnioserosa anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

dorsal ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

dorsal epidermis primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 14

somatic trunk mesoderm

(Bonn et al., 2013)

visceral trunk mesoderm

(Bonn et al., 2013)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

embryonic stage 14

muscle founder cell

(Bonn et al., 2013)

Additional Descriptive Data

Mhcl transcript expression begins at embryonic stage 11, in both visceral and somatic mesoderm. By stage 14, expression can be observed in muscle founder cells. At the end of embryogenesis, transcript is observed in segmental epidermal stripes, and in the esophagus. Mhcl transcript is not observed in mature myotubes.

(Bonn et al., 2013)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Guzik-Lendrum et al., 2011)

larval stage

larval somatic muscle cell

• Z disc

(Bonn et al., 2013)

adult stage

adult somatic muscle cell

• Z disc

(Bonn et al., 2013)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Mhcl protein immunoreactivity is localized immediately adjacent to the Z disc in the sarcomeres of larval and adult somatic muscles.

(Bonn et al., 2013)

A ubiquitous staining pattern is seen at all stages of embryonic development.

(Guzik-Lendrum et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in sarcomere

inferred from direct assay

(Bonn et al., 2013)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mhcl in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mhcl

View exonic expression by tissue for Dmel\Mhcl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026059

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41955/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026059

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026059

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026059

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31045

Flygut - An atlas of the Drosophila adult midgut

FBgn0026059

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Mhcl

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mhcl

Transgenic constructs containing regulatory region of Mhcl

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)ΔMhcl

(Bonn et al., 2013)

Df(3R)Exel7328

(Ryder, 2004.4.26)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (62)

Hsap\MYO18A

14 of 14

Yes

Hsap\MYO18B

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\CEP85

1 of 14

Yes

Hsap\CEP85L

1 of 14

Yes

Hsap\CFAP57

1 of 14

Hsap\CGN

1 of 14

Yes

Hsap\CGNL1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (38)

Rnor\Myo18a

13 of 14

Yes

Rnor\Myo18b

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Rnor\Cep85

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tamalin

1 of 14

Rnor\Ush1c

1 of 14

Rnor\Whrn

1 of 14

Mus musculus (laboratory mouse) (43)

Mmus\Myo18a

14 of 14

Yes

Mmus\Myo18b

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Mmus\Cep85

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tamalin

1 of 14

Mmus\Ush1c

1 of 14

Mmus\Whrn

1 of 14

Xenopus tropicalis (Western clawed frog) (23)

Xtro\myo18b

6 of 13

Yes

Xtro\adcyap1r1

1 of 13

Yes

Xtro\adra2b

1 of 13

Xtro\cbl

1 of 13

Xtro\cep85

1 of 13

Yes

Xtro\cgnl1

1 of 13

Yes

Xtro\cgn

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\spring1

1 of 13

Xtro\thra

1 of 13

Yes

Xtro\tmem229b

1 of 13

Yes

Xtro\tpmt

1 of 13

Yes

Danio rerio (Zebrafish) (46)

Drer\myo18aa

14 of 14

Yes

Drer\myo18ab

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Drer\cep85l

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC100537963

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\tamalin

1 of 14

Drer\ush1c

1 of 14

Drer\whrna

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (31)

Cele\nmy-3

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Cele\C50F7.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\F28E10.4

1 of 14

Yes

Cele\F40F9.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\myo-6

1 of 14

Cele\smz-1

1 of 14

Yes

Cele\smz-2

1 of 14

Yes

Cele\spe-15

1 of 14

Cele\T19B10.5

1 of 14

Yes

Cele\txt-1

1 of 14

Yes

Cele\unc-15

1 of 14

Cele\ZK849.1

1 of 14

Anopheles gambiae (African malaria mosquito) (29)

Agam\AgaP_AGAP011138

12 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012008

1 of 12

Agam\AgaP_AGAP012354

1 of 12

Arabidopsis thaliana (thale-cress) (17)

Atha\ATM1

1 of 13

Yes

Atha\ATM2

1 of 13

Yes

Atha\MYA1

1 of 13

Yes

Atha\MYA2

1 of 13

Yes

Atha\VIIIA

1 of 13

Yes

Atha\VIIIB

1 of 13

Yes

Atha\XIA

1 of 13

Yes

Atha\XIB

1 of 13

Yes

Atha\XIC

1 of 13

Yes

Atha\XID

1 of 13

Yes

Atha\XIE

1 of 13

Yes

Atha\XIF

1 of 13

Yes

Atha\XIG

1 of 13

Yes

Atha\XIH

1 of 13

Yes

Atha\XI-I

1 of 13

Yes

Atha\XIJ

1 of 13

Yes

Atha\XIK

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\MYO1

3 of 13

Yes

Scer\MYO2

1 of 13

Scer\MYO4

1 of 13

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\myo2

3 of 12

Yes

Spom\myp2

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mhcl. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (39)

Mhc

4 of 13

zip

4 of 13

Prm

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

baz

1 of 13

bbg

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

jar

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MYO18B; myosin XVIIIB

model of Klippel-Feil syndrome 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYO18A; myosin XVIIIA

14 of 14

609517, 610067

MYO18B; myosin XVIIIB

8 of 14

607295

KLIPPEL-FEIL SYNDROME 4, AUTOSOMAL RECESSIVE, WITH NEMALINE MYOPATHY AND FACIAL DYSMORPHISM; KFS4

Klippel-Feil syndrome 4

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mhcl

two hybrid

(Bonn et al., 2013)

Mhcl - AhcyL2

experimental knowledge based

(Guruharsha et al., 2011)

Mhcl - Rab5

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Mhcl - Rab6

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Mhcl - rols

two hybrid

(Bonn et al., 2013)

Mhcl - ssp

experimental knowledge based

(Guruharsha et al., 2011)

Mhcl - vers

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67007

DroID - A comprehensive database of gene and protein interactions.

FBgn0026059

MIST (protein-protein) - An integrated Molecular Interaction Database

41955

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q0KI67

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-58

Cytogenetic map

89B2-89B3

Sequence location

3R:16,068,523..16,098,761 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89B2-89B3

Limits computationally determined from genome sequence between P{PZ}blp⁰¹⁶¹⁸ and P{PZ}gish⁰⁴⁸⁹⁵&P{EP}EP3171

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

89B-89B

(Biru, 1999.1.28)

Experimentally Determined Recombination Data

Location

3-58

(FlyBase, 2016)

3-58.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

51456

y¹ v¹; P{TRiP.HMC03191}attP40

62973

y¹ v¹; P{TRiP.HMJ30050}attP40/CyO

26362

w¹¹¹⁸; Mi{ET1}Mhcl^MB08285 CG32855^MB08285

Kyoto

104044

y^* w^*; P{GawB}Mhcl^NP1604 / TM6, P{UAS-lacZ.UW23-1}UW23-1

NIG-Fly

HMC03191

y¹ v¹; P{TRiP.HMC03191}attP40/CyO

HMJ30050

y¹ v¹; P{TRiP.HMJ30050}attP40/CyO

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (51)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

IP18260

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0026059

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0026059

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Bonn et al., 2013, Guzik-Lendrum et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

(Rogers et al., 2003)

Identified with: LP08646 <up>FlyBase curator comment: EST subsequently found to be chimeric</up>.

(BDGP Project Members, 2000-)

FlyBase curator comment: DGC clone appears problematic: chimeric (contains Mhcl and Zeelin1 sequences).

(FlyBase, 1996-)

Relationship to Other Genes

Source for database merge of

Source for merge of: Mhcl CG10224

(Misra, 2001.8.10)

Source for merge of: Myosin-heavy-chain-like CG10224

(FlyBase, 1996-)

Additional comments

Annotations CG10218, CG10224 merged as CG31045 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of Myosin-heavy-chain-like CG10224 was a shared cDNA ( date:010720 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

CG10218

(Rogers et al., 2003, Berg et al., 2001, Goldstein and Gunawardena, 2000)

CG10224

(Stanyon et al., 2004, Misra, 2001.8.10)

CG31045

(Çiçek et al., 2016, Guzik-Lendrum et al., 2011, Ni et al., 2010.12.1, Perkins et al., 2009)

DmPDZ

(Yamashita et al., 2000)

MHCL(PDZ)

(Yamashita et al., 2000)

Mhcl

(Pang et al., 2021, Aboukilila et al., 2020, David-Morrison et al., 2016, Ashwal-Fluss et al., 2014, Luck et al., 2014, Bonn et al., 2013, Rodriguez et al., 2012, Guzik-Lendrum et al., 2011, Levis et al., 2011.12.16, Wasbrough et al., 2010)

Myo7A

(Kollmar, 2015.9.15)

Name Synonyms

Myosin XVIII

(Rogers et al., 2003)

Myosin heavy chain-like

(Trivedi et al., 2022, Yamashita et al., 2000)

Myosin-heavy-chain-like

PDZ-myosin

(Rogers et al., 2003, Berg et al., 2001)

myosin 18

(Guzik-Lendrum et al., 2011)

myosin XVIII (PDZ)

(Rogers et al., 2003)

myosin heavy chain

(Biru, 1999.1.28)

Secondary FlyBase IDs

FBgn0038399
FBgn0051045

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 94 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41955

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q8INC3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q8INC3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026059

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41955/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026059

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026059

FlyMine - An integrated database for Drosophila genomics

FBgn0026059

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG31045

MARRVEL_MODEL - MARRVEL (model organism gene)

41955

Linkouts

BioGRID - A database of protein and genetic interactions.

67007

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0026059

DroID - A comprehensive database of gene and protein interactions.

FBgn0026059

DRSC - Results frm RNAi screens

FBgn0026059

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026059

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0026059

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG31045

Flygut - An atlas of the Drosophila adult midgut

FBgn0026059

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030896

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Motor proteins

MIST (protein-protein) - An integrated Molecular Interaction Database

41955

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q0KI67

References (59)

The following external sites may use different assemblies or annotations than FlyBase.

-0.85

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