FlyBase Gene Report: Dmel\Fife

General Information

Symbol

Dmel\Fife

Species

D. melanogaster

Name

Fife

Annotation Symbol

CG43955

Feature Type

protein_coding_gene

FlyBase ID

FBgn0264606

Gene Model Status

Current

Stock Availability

29 publicly available

Gene Summary

Fife (Fife) encodes a synaptic scaffolding protein that promotes neurotransmitter release and normal motor behavior. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

62E8-62F1

Sequence location

3L:2,655,415..2,711,690 [-]

Recombination map

3-4

RefSeq locus

NT_037436 REGION:2655415..2711690

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables small GTPase binding

inferred from electronic annotation with InterPro:IPR039032

(InterPro Project Members, 2004-)

enables structural constituent of presynaptic active zone

inferred from biological aspect of ancestor with PANTHER:PTN000255280

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in positive regulation of neurotransmitter secretion

inferred from mutant phenotype

(Bruckner et al., 2012)

involved_in positive regulation of synaptic vesicle clustering

inferred from mutant phenotype

(Bruckner et al., 2012)

involved_in presynaptic active zone assembly

inferred from mutant phenotype

(Bruckner et al., 2017)

involved_in synaptic vesicle clustering

inferred from mutant phenotype

(Bruckner et al., 2017)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in exocytosis

inferred from electronic annotation with InterPro:IPR039032

(InterPro Project Members, 2004-)

involved_in regulation of synaptic vesicle exocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000255280

(GO Reference Genome Project, 2011-)

involved_in synaptic vesicle docking

inferred from biological aspect of ancestor with PANTHER:PTN000255280

(GO Reference Genome Project, 2011-)

involved_in synaptic vesicle priming

inferred from biological aspect of ancestor with PANTHER:PTN000255280

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoskeleton of presynaptic active zone

inferred from direct assay

(Bruckner et al., 2017)

located_in presynaptic active zone

inferred from direct assay

(Bruckner et al., 2012)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR039032

(InterPro Project Members, 2004-)

is_active_in presynaptic active zone cytoplasmic component

inferred from biological aspect of ancestor with PANTHER:PTN000255280

(GO Reference Genome Project, 2011-)

is_active_in presynaptic membrane

inferred from biological aspect of ancestor with PANTHER:PTN000255280

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Fife (Fife) encodes a synaptic scaffolding protein that promotes neurotransmitter release and normal motor behavior. [Date last reviewed: 2019-03-07]

Summary (Interactive Fly)

a presynaptic scaffolding protein of the neuromuscular junction - active zone cytomatrix - homeostatic modulation of neurotransmission - organizes active zones to create synaptic vesicle release sites

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Fife for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9PE32)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Fife-RB

FBtr0333629

NM_001274409

7459

969

Fife-RD

FBtr0333632

NM_001274411

6995

1314

Fife-RF

FBtr0333630

NM_001274410

5131

643

Fife-RG

FBtr0333631

NM_139485

5125

643

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Fife-PB

FBpp0305804

104.8

969

8.28

FBpp0305807

142.8

1314

9.30

FBpp0305805

68.4

643

8.66

FBpp0305806

68.4

643

8.66

Q9VZY3

NP_647742

AAF47680

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

643 aa isoforms: Fife-PF, Fife-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fife using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fife_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.73

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

neuron of larval central nervous system

(Bruckner et al., 2012)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoskeleton of presynaptic active zone

inferred from direct assay

(Bruckner et al., 2017)

located_in presynaptic active zone

inferred from direct assay

(Bruckner et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Fife in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Fife

View exonic expression by tissue for Dmel\Fife

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264606

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38337/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264606

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264606

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12187

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Fife

Transgenic constructs containing regulatory region of Fife

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6091

(Ryder, 2004.4.26)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Mef2.PR

Fife^GD14847

lethal, with Scer\GAL4^pnr-MD237

Fife^GD7522

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Fife^GD7522

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Fife^GD14847

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Fife^GD14847

Sterility

Allele

female sterile

Fife^ex1027

female sterile (with Df(3L)BSC412)

Fife^ex1027

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage

Fife^ex1027

abnormal locomotor behavior | adult stage (with Df(3L)BSC412)

Fife^ex1027

abnormal neuroanatomy | third instar larval stage

Fife^ex1027

abnormal neuroanatomy | third instar larval stage (with Df(3L)BSC412)

Fife^ex1027

abnormal neuroanatomy | third instar larval stage (with Df(3L)Exel6091)

abnormal neurophysiology | third instar larval stage

abnormal neurophysiology | third instar larval stage (with Df(3L)BSC412)

Fife^ex1027

abnormal neurophysiology | third instar larval stage (with Df(3L)Exel6091)

abnormal neurophysiology | third instar larval stage (with Fife^AC)

Fife^ex1027

abnormal neurophysiology | third instar larval stage (with Fife^ex1027)

Fife^AC

some die during pupal stage, with Scer\GAL4^pnr-MD237

Fife^GD14847

visible, with Scer\GAL4^pnr-MD237

Fife^GD14847

Phenotype manifest in

Allele

embryonic/larval neuromuscular junction | third instar larval stage

embryonic/larval neuromuscular junction | third instar larval stage (with Df(3L)Exel6091)

embryonic/larval neuromuscular junction | third instar larval stage (with Fife^AC)

Fife^ex1027

embryonic/larval neuromuscular junction | third instar larval stage (with Fife^ex1027)

Fife^AC

mesonotum, with Scer\GAL4^pnr-MD237

Fife^GD14847

presynaptic active zone

Fife^ex1027

presynaptic active zone (with Df(3L)BSC412)

Fife^ex1027

presynaptic active zone | third instar larval stage (with Df(3L)Exel6091)

presynaptic membrane

Fife^ex1027

presynaptic membrane (with Df(3L)BSC412)

Fife^ex1027

synapse | third instar larval stage (with Df(3L)Exel6091)

synaptic vesicle | third instar larval stage (with Df(3L)Exel6091)

Fife^AC

t-bar | third instar larval stage (with Df(3L)Exel6091)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (50)

Hsap\RIMS1

3 of 14

Yes

Hsap\RIMS2

3 of 14

Yes

Hsap\RIMS3

3 of 14

Yes

Hsap\RIMS4

3 of 14

Yes

Hsap\PCLO

2 of 14

Yes

Hsap\BSN

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (34)

Rnor\Rims1

3 of 14

Yes

Rnor\Rims2

3 of 14

Yes

Rnor\Rims3

3 of 14

Yes

Rnor\Rims4

3 of 14

Yes

Rnor\Bsn

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (37)

Mmus\Rims2

3 of 14

Yes

Mmus\Rims3

3 of 14

Yes

Mmus\Rims4

3 of 14

Yes

Mmus\Pclo

2 of 14

Yes

Mmus\Rims1

2 of 14

Mmus\Bsn

1 of 14

Yes

Mmus\Cytip

1 of 14

Mmus\Dlg5

1 of 14

Mmus\Gm20498

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (28)

Xtro\adcyap1r1

1 of 13

Yes

Xtro\adra2b

1 of 13

Yes

Xtro\cbl

1 of 13

Yes

Xtro\cep97

1 of 13

Yes

Xtro\fam81b

1 of 13

Yes

Xtro\LOC100486361

1 of 13

Yes

Xtro\LOC100493529

1 of 13

Yes

Xtro\med16

1 of 13

Yes

Xtro\pard6g

1 of 13

Yes

Xtro\pclo

1 of 13

Yes

Xtro\prkcb

1 of 13

Yes

Xtro\rasal1

1 of 13

Yes

Xtro\rgs18

1 of 13

Yes

Xtro\rims1

1 of 13

Yes

Xtro\rph3a

1 of 13

Yes

Xtro\spring1

1 of 13

Yes

Xtro\syt1

1 of 13

Yes

Xtro\syt2

1 of 13

Yes

Xtro\syt3

1 of 13

Yes

Xtro\syt7

1 of 13

Yes

Xtro\syt8

1 of 13

Yes

Xtro\syt9

1 of 13

Yes

Xtro\syt17

1 of 13

Yes

Xtro\sytl1

1 of 13

Yes

Xtro\sytl2

1 of 13

Yes

Xtro\sytl4

1 of 13

Yes

Xtro\sytl5

1 of 13

Yes

Xtro\thra

1 of 13

Yes

Danio rerio (Zebrafish) (40)

Drer\pcloa

3 of 14

Yes

Drer\rims1b

3 of 14

Yes

Drer\rims2b

3 of 14

Yes

Drer\rims3

3 of 14

Yes

Drer\rims4

3 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (20)

Cele\unc-10

2 of 14

Yes

Cele\C46H11.6

1 of 14

Yes

Cele\C50F7.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\F28E10.4

1 of 14

Yes

Cele\F40F9.3

1 of 14

Yes

Cele\gras-1

1 of 14

Cele\mics-1

1 of 14

Cele\mpz-4

1 of 14

Yes

Cele\pkc-2

1 of 14

Cele\smz-1

1 of 14

Yes

Cele\smz-2

1 of 14

Yes

Cele\snt-2

1 of 14

Cele\snt-4

1 of 14

Cele\T19B10.5

1 of 14

Yes

Cele\txt-1

1 of 14

Yes

Cele\ZK849.1

1 of 14

Anopheles gambiae (African malaria mosquito) (29)

Agam\AgaP_AGAP003604

8 of 12

Yes

Agam\AgaP_AGAP003603

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP012008

1 of 12

Agam\AgaP_AGAP012252

1 of 12

Agam\AgaP_AGAP012354

1 of 12

Arabidopsis thaliana (thale-cress) (1)

Atha\AT1G20130

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Fife. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (33)

Rim

2 of 13

1 of 13

baz

1 of 13

bbg

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

RIMS1; regulating synaptic membrane exocytosis 1

3 of 14

606629

RIMS2; regulating synaptic membrane exocytosis 2

3 of 14

606630

CONE-ROD SYNAPTIC DISORDER SYNDROME, CONGENITAL NONPROGRESSIVE; CRSDS

RIMS3; regulating synaptic membrane exocytosis 3

3 of 14

611600

RIMS4; regulating synaptic membrane exocytosis 4

3 of 14

611601

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Fife - Rbp

two hybrid

(Petzoldt et al., 2020)

Fife - Rim

anti tag coimmunoprecipitation, anti tag western blot

(Bruckner et al., 2017)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0264606

MIST (protein-protein) - An integrated Molecular Interaction Database

38337

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q86NW3

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-4

Cytogenetic map

62E8-62F1

Sequence location

3L:2,655,415..2,711,690 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

62E8-62F1

Limits computationally determined from genome sequence between P{PZ}Spn⁰⁶⁹¹¹ and P{PZ}l(3)06803⁰⁶⁸⁰³

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-4

(FlyBase, 2016)

3-5.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (29)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

71626

w¹¹¹⁸; P{VT024610-p65.AD}attP40

71878

w¹¹¹⁸; P{VT024604-p65.AD}attP40

73035

w¹¹¹⁸; P{VT024602-p65.AD}attP40

74575

w¹¹¹⁸; P{VT024604-GAL4.DBD}attP2

Korea

12395

P{VT024602-GAL4}attP2

12651

P{VT024603-GAL4}attP2

12652

P{VT024610-GAL4}attP2

13728

P{VT024604-GAL4}attP2

12187R-4

14950R-3

v103267

v110099

More stocks available...

Genomic Clones (38)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (21)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH19777

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0264606

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG12187 CG16976

(FlyBase Genome Annotators, 2011)

Source for merge of: CG12187 anon- EST:Posey171

(Ashburner, 2004.6.30)

Source for merge of: CG43375 CG14950

(FlyBase Genome Annotators, 2012)

Source for merge of: CG12187 CG16976 CG14950

(Bruckner et al., 2012)

Additional comments

Annotations CG43375 and CG14950 merged as CG43955 in release 5.46 of the genome annotation.

(FlyBase Genome Annotators, 2012)

Annotations CG12187 and CG16976 merged as CG43375 in release 5.37 of the genome annotation.

(FlyBase Genome Annotators, 2011)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "fife" to reflect its homology to Piccolo-RIM proteins and its smaller size relative to Piccolo.

(Bruckner et al., 2012)

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG12187

(Aradska et al., 2015, Ashwal-Fluss et al., 2014, Bruckner et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5)

CG14950

(Bruckner et al., 2012, Japanese National Institute of Genetics, 2012.5.21)

CG16976

(Ashwal-Fluss et al., 2014, Bruckner et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Keleman et al., 2009.8.5, Holloway et al., 2008, Adolfsen and Littleton, 2001)

CG43375

(Rodriguez et al., 2012, FlyBase Genome Annotators, 2011)

CG43955

(Aranjuez et al., 2012, Bruckner et al., 2012, FlyBase Genome Annotators, 2012)

Fife

(Tsakiri et al., 2025, Hofbauer et al., 2024, Duan et al., 2018, Shearin et al., 2018, Bruckner et al., 2017, Duun Rohde et al., 2016, Gene Disruption Project members, 2016-)

anon-EST:Posey171

fife

(Frank et al., 2020, Petzoldt et al., 2020, Wentzel et al., 2018, Bruckner et al., 2012)

Name Synonyms

Fife

Secondary FlyBase IDs

FBgn0035366
FBgn0027741
FBgn0035367
FBgn0263177
FBgn0035364

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38337

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9PE32

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9PE32

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0264606

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38337/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0264606

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0264606

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG43955

MARRVEL_MODEL - MARRVEL (model organism gene)

38337

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0264606

DroID - A comprehensive database of gene and protein interactions.

FBgn0264606

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fife_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0264606

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12187

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0264606

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032593

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

38337

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q86NW3

References (56)

The following external sites may use different assemblies or annotations than FlyBase.

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