FlyBase Gene Report: Dmel\Pi3K68D

General Information

Symbol

Dmel\Pi3K68D

Species

D. melanogaster

Name

Phosphatidylinositol 3-kinase 68D

Annotation Symbol

CG11621

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015278

Gene Model Status

Current

Stock Availability

21 publicly available

Enzyme Name (EC)

phosphatidylinositol 3-kinase (2.7.1.137)

phosphatidylinositol-4-phosphate 3-kinase (2.7.1.154)

Gene Summary

Phosphotidylinositol 3 kinase 68D (Pi3K68D) encodes a phosphatidylinositol kinase involved in the organization of the cortical actin cytoskeleton and the regulation of the Egfr signaling pathway. [Date last reviewed: 2019-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

PI3K, PI3K_68D, dPI3K, cpk, PI(3)K

Key Links

Genomic Location

Cytogenetic map

68D4-68D6

Sequence location

3L:11,774,650..11,790,013 [-]

Recombination map

3-36

RefSeq locus

NT_037436 REGION:11774650..11790013

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

enables 1-phosphatidylinositol-3-kinase activity

inferred from direct assay

(MacDougall et al., 2004, MacDougall et al., 1995)

enables 1-phosphatidylinositol-4-phosphate 3-kinase activity

inferred from direct assay

(MacDougall et al., 1995)

enables phospholipid binding

inferred from direct assay

(MacDougall et al., 1995)

enables protein binding

inferred from physical interaction with FLYBASE:Sbf; FB:FBgn0025802

(Jean et al., 2012)

enables SH3 domain binding

inferred from physical interaction with FLYBASE:drk; FB:FBgn0004638

(MacDougall et al., 2004)

enables small GTPase binding

inferred from physical interaction with FLYBASE:Rab21; FB:FBgn0039966

(Jean et al., 2012)

NOT enables 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity

inferred from direct assay

(MacDougall et al., 1995)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables 1-phosphatidylinositol-3-kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000005674

(GO Reference Genome Project, 2011-)

enables 1-phosphatidylinositol-4-phosphate 3-kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000005674

(GO Reference Genome Project, 2011-)

enables kinase activity

inferred from electronic annotation with InterPro:IPR018936

(InterPro Project Members, 2004-)

enables phosphatidylinositol binding

inferred from electronic annotation with InterPro:IPR001683, InterPro:IPR036871

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in cortical actin cytoskeleton organization

inferred from mutant phenotype

(Jean et al., 2012)

involved_in negative regulation of epidermal growth factor receptor signaling pathway

inferred from genetic interaction with FLYBASE:Egfr; FB:FBgn0003731

(MacDougall et al., 2004)

inferred from genetic interaction with FLYBASE:aos; FB:FBgn0004569

(MacDougall et al., 2004)

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from direct assay

(MacDougall et al., 1995)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in cell migration

inferred from biological aspect of ancestor with PANTHER:PTN000005674

(GO Reference Genome Project, 2011-)

involved_in phosphatidylinositol 3-kinase/protein kinase B signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN000005674

(GO Reference Genome Project, 2011-)

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from electronic annotation with InterPro:IPR015433

(InterPro Project Members, 2004-)

involved_in phosphatidylinositol-3-phosphate biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000005674

(GO Reference Genome Project, 2011-)

involved_in phosphatidylinositol-mediated signaling

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in endosome membrane

inferred from direct assay

(Jean et al., 2012)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000005674

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

PHOSPHATIDYLINOSITOL 3 KINASES

Pathway (FlyBase)

NEGATIVE REGULATORS OF EGFR SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

1-phosphatidylinositol-3-kinase activity

a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl- sn-glycero-3-phospho-(1D-myo-inositol-3-phosphate) + ADP + H(+) (2.7.1.137)

RHEA 12709: 1-phosphatidylinositol-4-phosphate 3-kinase activity

a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3,4-bisphosphate) + ADP + H(+) (2.7.1.154)

RHEA 18373:

Summaries

Gene Snapshot

Pathway (FlyBase)

NEGATIVE REGULATORS OF EGFR SIGNALING PATHWAY -: Negative regulators of Epidermal Growth Factor Receptor signaling down-regulate the pathway, suppressing the activation of ERK kinase (rl) or acting on downstream effectors.

Gene Group (FlyBase)

PHOSPHATIDYLINOSITOL 3 KINASES -: Phosphatidylinositol 3 kinases catalyze the phosphorylation of phosphatidylinositol (PI), or specific mono or bisphosphate derivatives of PI, at position 3 on the inositol ring to generate PI3P, PI(3,4)P[[2]] or PI(3,4,5)P[[3]]. The resulting phosphoinositides can act as signalling molecules to mediate/regulate a range of cellular and physiological processes, including cell polarity, cell migration, autophagy, growth, development, and ageing. (Adapted from FBrf0227909.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pi3K68D for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7K3H0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pi3K68D-RA

FBtr0076088

NM_079304

7211

1876

Pi3K68D-RB

FBtr0076086

NM_168476

1880

366

Pi3K68D-RC

FBtr0076087

NM_168475

6910

1876

Pi3K68D-RD

FBtr0301816

NM_001169949

6862

1876

Pi3K68D-RE

FBtr0332055

NM_001274795

2181

366

Additional Transcript Data and Comments

Reported size (kB)

6.831 (longest cDNA)

(Molz et al., 1996)

7.215 (compiled cDNA)

(MacDougall et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pi3K68D-PA

FBpp0075819

210.5

1876

6.66

FBpp0075817

39.7

366

7.52

FBpp0075818

210.5

1876

6.66

FBpp0291030

210.5

1876

6.66

FBpp0304365

39.7

366

7.52

Q95RR5

NP_001261724

AGB94418

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1876 aa isoforms: Pi3K68D-PA, Pi3K68D-PC, Pi3K68D-PD

366 aa isoforms: Pi3K68D-PB, Pi3K68D-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

1876 (aa); 210 (kD observed); 210 (kD predicted)

(Molz et al., 1996)

1876 (aa); 210 (kD predicted)

(MacDougall et al., 1995)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pi3K68D using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pi3K68D_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.29

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic/larval midgut

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

enzyme assay or biochemical detection

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage -- adult stage

(MacDougall et al., 1995)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Pi3K68D is widely expressed throughout the Drosophila life cycle.

(MacDougall et al., 1995)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in endosome membrane

inferred from direct assay

(Jean et al., 2012)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.2}Pi3K68D^{MI15179-TG4.2}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult brain | restricted

(Lee et al., 2018, Lee et al., 2018)

neuron | subset of adult pars intercerebralis

(Lee et al., 2018)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pi3K68D in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pi3K68D

View exonic expression by tissue for Dmel\Pi3K68D

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015278

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39329/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015278

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015278

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015278

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0015278

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 18 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pi3K68D

Transgenic constructs containing regulatory region of Pi3K68D

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene not disrupted in

Df(3L)vin2

(MacDougall et al., 2004)

Df(3L)vin66

(MacDougall et al., 2004)

Gene disrupted in

Df(3L)vin3

(MacDougall et al., 2004)

Df(3L)vin4

(MacDougall et al., 2004)

Df(3L)vin6

(MacDougall et al., 2004)

Df(3L)vin7

(MacDougall et al., 2004)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^C57

Pi3K68D^UAS.EGFP

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Pi3K68D^GD7348

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pi3K68D^GD7348

viable, with Scer\GAL4^Mef2.PR

Pi3K68D^GD7348

viable, with Scer\GAL4^tin.CΔ4

Pi3K68D^GD7348

Other Phenotypes

Allele

abnormal neurophysiology | larval stage

abnormal neurophysiology | larval stage, with Pi3K68D^AH1, Scer\GAL4^C57

Pi3K68D^UAS.EGFP

abnormal neurophysiology | larval stage, with Pi3K68D^UAS.EGFP, Scer\GAL4^C57

Pi3K68D^AH1

abnormal neurophysiology | larval stage, with Scer\GAL4^C57

Pi3K68D^UAS.EGFP

abnormal neurophysiology | larval stage, with Scer\GAL4^C57, Scer\GAL4^sca.PU

Pi3K68D^HMS01296

flightless, with Scer\GAL4^Mef2.PR

Pi3K68D^GD7348

some die during pupal stage, with Scer\GAL4^pnr-MD237

Pi3K68D^GD7348

visible, with Scer\GAL4^69B

visible, with Scer\GAL4^Bx-MS1096

Pi3K68D^UAS.Tag:MYC

visible, with Scer\GAL4^ptc-559.1

Phenotype manifest in

Allele

campaniform sensillum | ectopic, with Scer\GAL4^69B

Pi3K68D^{KD.UAS.Tag:MYC}

chaeta | increased number, with Scer\GAL4^69B

Pi3K68D^{KD.UAS.Tag:MYC}

embryonic/larval hemocyte, with Scer\GAL4^Pxn.PS

Pi3K68D^UAS.EGFP

embryonic/larval neuromuscular junction | larval stage

embryonic/larval neuromuscular junction | larval stage, with Pi3K68D^AH1, Scer\GAL4^C57

Pi3K68D^UAS.EGFP

embryonic/larval neuromuscular junction | larval stage, with Pi3K68D^UAS.EGFP, Scer\GAL4^C57

Pi3K68D^AH1

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^C57

Pi3K68D^UAS.EGFP

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^C57, Scer\GAL4^sca.PU

Pi3K68D^HMS01296

embryonic/larval plasmatocyte, with Scer\GAL4^Cg.PA

Pi3K68D^GD7348

endolysosome, with Scer\GAL4^He.PZ

Pi3K68D^UAS.mCherry

first basal wing cell | proximal, with Scer\GAL4^69B

Pi3K68D^UAS.Tag:MYC

haltere, with Scer\GAL4^69B

Pi3K68D^UAS.Tag:MYC

hemocyte, with Scer\GAL4^He.PZ

Pi3K68D^UAS.mCherry

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

Pi3K68D^GD7348

larval somatic muscle cell, with Scer\GAL4^C57

myofibril, with Scer\GAL4^Mef2.PR

Pi3K68D^GD7348

sarcomere, with Scer\GAL4^Mef2.PR

Pi3K68D^GD7348

scutellar bristle, with Scer\GAL4^ptc-559.1

scutellar bristle | increased number, with Scer\GAL4^ptc.PU

Pi3K68D^{KDΔ21.UAS.mCherry}

scutellum, with Scer\GAL4^ptc-559.1

Pi3K68D^UAS.Tag:MYC

wing, with Scer\GAL4^69B

Pi3K68D^UAS.Tag:MYC

wing, with Scer\GAL4^Bx-MS1096

Pi3K68D^UAS.Tag:MYC

wing margin, with Scer\GAL4^69B

Pi3K68D^{KD.UAS.Tag:MYC}

wing vein | ectopic, with Scer\GAL4^69B

Pi3K68D^{KD.UAS.Tag:MYC}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (48)

Hsap\PIK3C2A

14 of 14

Yes

Hsap\PIK3C2B

13 of 14

Yes

Hsap\PIK3C2G

8 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\SH3PXD2B

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Pik3c2a

14 of 14

Yes

Rnor\Pik3c2b

13 of 14

Yes

Rnor\Pik3c2g

8 of 14

Yes

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (33)

Mmus\Pik3c2a

14 of 14

Yes

Mmus\Pik3c2b

12 of 14

Yes

Mmus\Pik3c2g

7 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Sh3pxd2b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (31)

Xtro\pik3c2a

8 of 13

Yes

Xtro\pik3c2b

2 of 13

Yes

Xtro\pik3ca

2 of 13

Xtro\alox12b

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (44)

Drer\pik3c2a

14 of 14

Yes

Drer\pik3c2b

13 of 14

Yes

Drer\pik3c2g

6 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-266g18.6

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\piki-1

14 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (18)

Agam\AgaP_AGAP011036

12 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (10)

Atha\VPS34

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (8)

Scer\MEC1

2 of 13

Yes

Scer\PIK1

2 of 13

Yes

Scer\STT4

2 of 13

Yes

Scer\TOR1

2 of 13

Yes

Scer\TOR2

2 of 13

Yes

Scer\VPS34

2 of 13

Yes

Scer\TEL1

1 of 13

Scer\TRA1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (9)

Spom\pik1

2 of 12

Yes

Spom\pik3

2 of 12

Yes

Spom\stt4

2 of 12

Yes

Spom\tor1

2 of 12

Yes

Spom\tor2

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pi3K68D. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

Pi3K59F

5 of 13

Pi3K92E

5 of 13

fwd

4 of 13

4 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Rim

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Pi3K68D^JF01193

ameliorates Charcot-Marie-Tooth disease type 4B1

modeled by mtm^G447D

(Hu et al., 2022)

Pi3K68D^GL00159

ameliorates Charcot-Marie-Tooth disease type 4B1

modeled by mtm^G447D

(Hu et al., 2022)

Pi3K68D^HMS01296

ameliorates Charcot-Marie-Tooth disease type 4B1

modeled by mtm^G447D

(Hu et al., 2022)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PIK3C2A; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha

14 of 14

603601

OCULOSKELETODENTAL SYNDROME; OCSKD

PIK3C2B; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 beta

13 of 14

602838

PIK3C2G; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 gamma

8 of 14

609001

PIK3CD; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta

4 of 14

PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, DELTA; PIK3CD

immunodeficiency 14

PIK3CG; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma

4 of 14

601232

IMMUNODEFICIENCY 97 WITH AUTOINFLAMMATION; IMD97

immunodeficiency 97

PIK3C3; phosphatidylinositol 3-kinase catalytic subunit type 3

3 of 14

602609

PIK3CB; phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta

3 of 14

PHOSPHATIDYLINOSITOL 3-KINASE, CATALYTIC, BETA; PIK3CB

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pi3K68D - 14-3-3ζ

anti tag coimmunoprecipitation, anti tag western blot

(Pueyo et al., 2016)

Pi3K68D - Ank

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Art4

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CG1371

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CG3008

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CG3902

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CG9467

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - CaBP1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Chc

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Cnx99A

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Fim

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Glt

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - GluProRS

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Gp210

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - HnRNP-K

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - LanB2

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - MRP

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Mcad

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Mpcp2

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Nacα

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Nfs1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Nup358

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Ost1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - OstΔ

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Past1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Pef

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Pih1D1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Pp2A-29B

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Pxn

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Rab21

anti tag coimmunoprecipitation, anti tag western blot

(Jean et al., 2012)

Pi3K68D - Rab5

anti tag coimmunoprecipitation, anti tag western blot

(Jean et al., 2012)

Pi3K68D - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - RpLP0

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - RpS10b

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - SERCA

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Sbf

anti bait coimmunoprecipitation, western blot, anti tag western blot

(Jean et al., 2012)

Pi3K68D - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - SerRS

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - TER94

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - Vps4

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - boca

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - bor

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - cPges

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - drk

pull down, western blot

(MacDougall et al., 2004)

Pi3K68D - fax

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - hale

two hybrid

(Hu et al., 2017.6.13)

Pi3K68D - kst

experimental knowledge based, pull down, western blot

(Guruharsha et al., 2011, MacDougall et al., 2004)

Pi3K68D - mEFTu1

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - mod

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - mtm

anti bait coimmunoprecipitation, western blot

(Jean et al., 2012)

Pi3K68D - r

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - scf

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - vkg

experimental knowledge based

(Guruharsha et al., 2011)

Pi3K68D - α-Spec

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Pi3K68D

suppressible

14-3-3ζ

(Pueyo et al., 2016)

Pi3K68D

suppressible

drk

(MacDougall et al., 2004)

Pi3K68D

enhanceable

Egfr

(MacDougall et al., 2004)

Pi3K68D

suppressible

hemo

(Pueyo et al., 2016)

Pi3K68D

enhanceable

(Hauswirth et al., 2018)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

14-3-3ζ

suppressible

Pi3K68D

(Pueyo et al., 2016)

aos

suppressible

Pi3K68D

(MacDougall et al., 2004)

aos

enhanceable

Pi3K68D

(MacDougall et al., 2004)

suppressible

(Hu et al., 2022)

suppressible

(Hu et al., 2022)

suppressible

(Hauswirth et al., 2018)

mtm

suppressible

Pi3K68D

(Hu et al., 2022, Jean et al., 2012, Ribeiro et al., 2011, Velichkova et al., 2010)

enhanceable

Pi3K68D

(MacDougall et al., 2004)

Sbf

suppressible

Pi3K68D

(Jean et al., 2012)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0015278

MIST (genetic) - An integrated Molecular Interaction Database

39329

MIST (protein-protein) - An integrated Molecular Interaction Database

39329

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF EGFR SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

3-phosphoinositide biosynthesis

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q7JNX6

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-36

Cytogenetic map

68D4-68D6

Sequence location

3L:11,774,650..11,790,013 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68D4-68D6

Limits computationally determined from genome sequence between P{PZ}l(3)01239⁰¹²³⁹&P{lacW}l(3)01239^j9B4 and P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

68D-68D

(Molz, 1996.3.22)

68D-68D

(determined by in situ hybridisation)

(MacDougall et al., 1995)

68D1-68D3

(determined by in situ hybridisation)

(MacDougall, 1995.10.6)

Experimentally Determined Recombination Data

Location

3-36

(FlyBase, 2016)

3-30.1

(Comeron et al., 2012)

(Molz, 1996.3.22)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31252

y¹ v¹; P{TRiP.JF01193}attP2

80897

y¹, v¹; P{TKO.GS04801}attP40

93739

w^*; Pi3K68D^KO/TM3, Sb¹

92489

y¹ v¹; P{U6.2-Pi3K68D.dgRNA}attP40

26363

w¹¹¹⁸; Mi{ET1}Pi3K68D^MB08286 CG14131^MB08286

11621R-1

11621R-2

v109582

v16240

w¹¹¹⁸; P{GD7348}v16240

v16239

w¹¹¹⁸; P{GD7348}v16239/CyO

v318696

PBac{fTRG00859.sfGFP-TVPTBF}VK00002

v342827

P{hsFLP}1, y1 w1118; P{HD_CFD02094}attP40/CyO-GFP

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (113)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015278

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015278

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Jean et al., 2012, MacDougall et al., 1995)

monoclonal

(Molz et al., 1996)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Isolated from a cDNA library by PCR, using degenerate primers based on conserved regions of phosphatidylinositol kinases.

(Molz et al., 1996)

A Pi3K68D cDNA has been cloned and sequenced.

(Molz et al., 1996)

Identified by PCR using degenerate primers based on regions of strong amino acid homology found within the putative lipid kinase domain of mammalian p110α and yeast Vps34p.

(MacDougall et al., 1995)

Relationship to Other Genes

Source for database merge of

Source for merge of: Pi3K68D BcDNA:LD15217

(FlyBase, 1996-)

Additional comments

Source for merge of Pi3K68D BcDNA:LD15217 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Pi3K68D CG11621

(Zhang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

11621

(Ravi et al., 2009)

BcDNA:LD15217

CG11621

(Hodge et al., 2021, Trivedi et al., 2020, Balakrishnan et al., 2015, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Ni et al., 2008, Qin et al., 2008, Morrison et al., 2000)

Cpk

(Vanhaesebroeck et al., 1997)

PI(3)K

(Fuss et al., 2007, Edgar, 1999, Lehner, 1999)

PI3K

(Wang et al., 2022, Wenner and Pekala, 2022, Gautam et al., 2021, Kannangara et al., 2021, Lam Wong and Verheyen, 2021, Morin-Poulard et al., 2021, Oliveira et al., 2021, Sciambra and Chtarbanova, 2021, Ding et al., 2020, Jasper, 2020, Lee et al., 2020, Mariano et al., 2020, Pan et al., 2020, Pastor-Pareja, 2020, Texada et al., 2020, Gupta and Ray, 2017, Nirala et al., 2013, Delanoue et al., 2010, Ahrens et al., 2007, Hoshizaki and Gibbs, 2007, Howlett et al., 2007, Lasko and Sonenberg, 2007, Lavery and Stern, 2007, Caldwell et al., 2006, Liu and Lehmann, 2006, Luo et al., 2006, Martin-Pena et al., 2006, Shiojima and Walsh, 2006, McNeill and Bateman, 2005, Scott et al., 2005, Grewal and Saucedo, 2004, Leopold, 2004, Dickson, 2003, Gumienny and Padgett, 2003, Li et al., 2003, Longo and Finch, 2003, Luque et al., 2003, Neufeld, 2003, Nijhout, 2003, Saucedo et al., 2003, Saucedo and Edgar, 2002, Gingras et al., 2001, Jünger et al., 2001, Tapon et al., 2001, Leevers, 1999, Stambolic et al., 1999)

PI3K 68D

(MacDougall, 1995.11.9)

PI3K 68_D

(Domin and Waterfield, 1997)

PI3K-68D

(Di Cara et al., 2015, MacDougall, 1995.10.6)

PI3K-68D/E

(MacDougall et al., 1998)

PI3K68D

(Ghosh et al., 2023, Hemalatha et al., 2016, Pueyo et al., 2016, Balakrishnan et al., 2015, Powis and Macdougall, 2011, Zhang et al., 2010, Ribeiro et al., 2008, Junion et al., 2007, Martin et al., 2007)

PI3K_68D

(Powis and Macdougall, 2011, MacDougall et al., 2004, Gagou and MacDougall, 2003, Gagou and MacDougall, 2003, MacDougall and Gagou, 2002, Gagou and MacDougall, 2001, Wymann and Pirola, 1998, Vanhaesebroeck et al., 1997, Leevers et al., 1996, MacDougall et al., 1995)

PI₃K

(Hardie, 2003)

Pi3K

(Cui et al., 2020, Malita and Rewitz, 2020, Tang et al., 2011, Edgar, 2006)

cpk

(Molz, 1996.3.22, Molz et al., 1996, Molz et al., 1996)

dPI3K

(Pospisilik et al., 2010, Oldham et al., 2005, Jorgensen and Tyers, 2004, Kim et al., 2004, Jacinto and Hall, 2003)

dPI3k

(Chen et al., 2015)

dPIK

(Molz and Williams, 1995)

pi3k68d

(Hu et al., 2022, Gupta et al., 2014)

Name Synonyms

PI 3-kinase

(Hardie, 2003, Day and Lawrence, 2000)

PI3-kinase

(Wilson and Leptin, 2000)

Phosphatidylinositol 3-kinase 68D

Phosphotidylinositol 3 kinase 68D

(Leevers et al., 1995)

dPI 3-kinase

(Marygold and Leevers, 2002)

Secondary FlyBase IDs

FBgn0047208

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 89 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39329

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7K3H0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7K3H0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015278

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39329/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015278

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015278

FlyMine - An integrated database for Drosophila genomics

FBgn0015278

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG11621

MARRVEL_MODEL - MARRVEL (model organism gene)

39329

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015278

DroID - A comprehensive database of gene and protein interactions.

FBgn0015278

DRSC - Results frm RNAi screens

FBgn0015278

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pi3K68D_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015278

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015278

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

3-phosphoinositide biosynthesis

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0015278

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0015278

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034925

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

39329

MIST (protein-protein) - An integrated Molecular Interaction Database

39329

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q7JNX6

References (168)

The following external sites may use different assemblies or annotations than FlyBase.

1.29

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