FlyBase Allele Report: Dmel\Pi3K68D[UAS.EGFP]

General Information

Symbol

Dmel\Pi3K68D^UAS.EGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0258486

Feature type

allele

Associated gene

Dmel\Pi3K68D

Associated Insertion(s)

Carried in Construct

P{UAS-Pi3K68D-eGFP}

Also Known As

UAS-Pi3K68D:eGFP, UAS-PI3K68D-GFP

Key Links

Transgenic product class

wild_type

(Velichkova et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Velichkova et al., 2010)

Mutagen

in vitro construct

(Velichkova et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Pi3K68D-eGFP}

Cytology

Description

UAS regulatory sequences drive expression of Pi3K68D which is tagged at the C-terminal end with EGFP.

(Velichkova et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Pi3K68D

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | larval stage, with Pi3K68D^AH1, Scer\GAL4^C57

(Hauswirth et al., 2018)

abnormal neurophysiology | larval stage, with Scer\GAL4^C57

(Hauswirth et al., 2018)

lethal, with Scer\GAL4^C57

(Hauswirth et al., 2018)

Phenotype Manifest In

embryonic/larval hemocyte, with Scer\GAL4^Pxn.PS

(Velichkova et al., 2010)

embryonic/larval neuromuscular junction | larval stage, with Pi3K68D^AH1, Scer\GAL4^C57

(Hauswirth et al., 2018)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^C57

(Hauswirth et al., 2018)

larval somatic muscle cell, with Scer\GAL4^C57

(Hauswirth et al., 2018)

Detailed Description

Statement

Reference

Third instar larvae expressing Pi3K68D^UAS.EGFP under the control of Scer\GAL4^VGlut-OK371 do not show apparent defects in neurotransmission across the neuromuscular junctions (mEPSP and EPSP amplitudes, and quantal content), as compared to controls. Expression under the control of Scer\GAL4^C57 impairs muscle health and leads to an apparent decrease in EPSP amplitude, but not in mEPSP amplitude or quantal content, as compared to controls; In a Pi3K68D^AH1 homozygous background the Scer\GAL4^C57-driven expression leads to a decreased mEPSP amplitude, as compared to controls.

(Hauswirth et al., 2018)

Primary hemocytes derived from larvae expressing Pi3K68D^{Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^Pxn.PS have enlarged endolysosomes.

(Velichkova et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Pi3K68D^{Scer\UAS.T:Avic\GFP-EGFP}

Pi3K68D^UAS.EGFP

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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