FlyBase Gene Report: Dmel\Pi4KIIIα

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\Pi4KIIIα

General Information

Symbol

Dmel\Pi4KIIIα

Species

D. melanogaster

Name

Phosphatidylinositol 4-kinase III α

Annotation Symbol

CG10260

Feature Type

protein_coding_gene

FlyBase ID

FBgn0267350

Gene Model Status

Stock Availability

13 publicly available

Enzyme Name (EC)

1-phosphatidylinositol 4-kinase (2.7.1.67)

Gene Summary

Phosphatidylinositol 4-kinase III α (PI4KIIIα) encodes a lipid kinase that synthesizes phosphatidylinositol 4-phosphate from phosphatidylinositol. Its roles include localization of plasma membrane-associated cytoskeletal and membrane trafficking proteins, Hippo signaling regulation and polarization of developing oocytes. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
Red Book
Interactive Fly

Also Known As

zw2, l(1)zw2, PI4K, EG:BACR7C10.2 , zw-2

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:2,624,804..2,633,672 [+]

Recombination map

1-1

RefSeq locus

NC_004354 REGION:2624804..2633672

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables 1-phosphatidylinositol 4-kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000006021

(GO Reference Genome Project, 2011-)

enables kinase activity

inferred from electronic annotation with InterPro:IPR018936

(InterPro Project Members, 2004-)

Biological Process (10 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in actin filament organization

inferred from mutant phenotype

(Tan et al., 2014)

involved_in basement membrane organization

inferred from mutant phenotype

(Devergne et al., 2014)

involved_in establishment of cell polarity

inferred from mutant phenotype

(Tan et al., 2014)

involved_in germarium-derived egg chamber formation

inferred from mutant phenotype

(Tan et al., 2014)

involved_in oogenesis

inferred from mutant phenotype

(Tan et al., 2014)

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from mutant phenotype

(Tan et al., 2014)

involved_in plasma membrane organization

inferred from mutant phenotype

(Tan et al., 2014)

involved_in positive regulation of smoothened signaling pathway

inferred from mutant phenotype

(Yavari et al., 2010)

involved_in regulation of synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Khuong et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in phosphatidylinositol phosphate biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR015433

(InterPro Project Members, 2004-)

involved_in phosphatidylinositol-mediated signaling

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000005673

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000006021

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

PHOSPHATIDYLINOSITOL 4 KINASES

Pathway (FlyBase)

POSITIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

1-phosphatidylinositol 4-kinase activity

a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl- sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP + H(+) (2.7.1.67)

RHEA 19877:

Summaries

Gene Snapshot

Phosphatidylinositol 4-kinase III α (PI4KIIIα) encodes a lipid kinase that synthesizes phosphatidylinositol 4-phosphate from phosphatidylinositol. Its roles include localization of plasma membrane-associated cytoskeletal and membrane trafficking proteins, Hippo signaling regulation and polarization of developing oocytes. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

PHOSPHATIDYLINOSITOL 4 KINASES -: Phosphatidylinositol 4 kinases catalyze the phosphorylation of phosphatidylinositol (PI) or specific mono phosphate derivatives of PI at position 4 on the inositol ring to generate PI4P, PI(3,4)P[[2]] or PI(4,5)P[[2]]. The resulting phosphoinositides can then act as signalling molecules to mediate/regulate a range of cellular and physiological processes, including cell polarity, cell migration, autophagy, growth, development, and ageing. (Adapted from FBrf0227909.)

Pathway (FlyBase)

POSITIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(1)3Ah

Lethal alleles [l(1)3Ah7, l(1)3Ah9, l(1)3Ah11] die at the egg-larval boundary; l(1)3Ah16 and l(1)3Ah12 die shortly after hatching. Cell viable, but gynandromorphs show reduced survival. l(1)3Ah6 semilethal with biphasic lethal expression in early larval and imago stages; escapers have rough eyes and are male fertile; inviable in combination with deficiency or lethal alleles (Shannon, Kaufman, Shen, and Judd, l972, Genetics 72: 615-38). l(1)3Ah13 viable in epidermal but not female germ-line clones (Garcia-Bellido and Robbins, 1983, Genetics 103: 235-47).

Gene Model and Products

Number of Transcripts

2

Number of Unique Polypeptides

2

Please see the JBrowse view of Dmel\Pi4KIIIα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9PDM4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.56

Gene model reviewed during 5.45

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0307158

6675

2134

FBtr0331587

7054

2154

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0297987

239.8

2134

7.91

FBpp0303977

242.0

2154

7.91

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pi4KIIIα using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

PI4KIIIalpha_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.07

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pi4KIIIα in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pi4KIIIα

View exonic expression by tissue for Dmel\Pi4KIIIα

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31247/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267350

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040335

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267350

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 133 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pi4KIIIα

Transgenic constructs containing regulatory region of Pi4KIIIα

Aberrations (Deficiencies and Duplications) ( 67 )

Inferred from experimentation ( 67 )

Gene duplicated in

(Kramers et al., 1991, Kramers et al., 1983, Judd et al., 1972)

(Judd et al., 1972)

(Lefevre, 1981)

(Judd et al., 1972)

(Eeken et al., 1985, Lefevre, 1981)

(Eeken et al., 1985)

(Yan et al., 2011)

Gene disrupted in

(Nomura and Kurokawa, 1997)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Bourouis et al., 1990, Lin and Wolfner, 1989, Perrimon and Smouse, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Kramers et al., 1983)

(Kramers et al., 1983)

(Perrimon and Smouse, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Nomura and Kurokawa, 1997)

(Kramers et al., 1991, Kramers et al., 1983, Lim and Snyder, 1974)

(Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983, Thierry-Mieg, 1982, Liu and Lim, 1975, Lim and Snyder, 1974)

(Lim and Snyder, 1974)

(Kramers et al., 1991, Kramers et al., 1983, Lim and Snyder, 1974)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Kramers et al., 1991, Kramers et al., 1983)

(Judd et al., 1972)

(Nomura and Kurokawa, 1997, Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983, Thierry-Mieg, 1982, Liu and Lim, 1975, Lim and Snyder, 1974, Judd et al., 1972)

(Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

Gene not disrupted in

(Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Bourouis et al., 1990, Lin and Wolfner, 1989, Perrimon and Smouse, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Thierry-Mieg, 1982, Judd et al., 1972)

(Nomura and Kurokawa, 1997)

(Nomura and Kurokawa, 1997)

(Kramers et al., 1991, Lin and Wolfner, 1989, Eeken et al., 1985)

(Kramers et al., 1991)

(Kramers et al., 1991)

(Kramers et al., 1991, Eeken et al., 1985)

(Schalet, 1986)

(Lim and Snyder, 1974)

(Kramers et al., 1991, Eeken et al., 1985, Kramers et al., 1983, Paradi et al., 1983, Liu and Lim, 1975)

(Kramers et al., 1991, Eeken et al., 1985, Kramers et al., 1983, Paradi et al., 1983, Liu and Lim, 1975, Lim and Snyder, 1974)

(Lin and Wolfner, 1989)

(Kramers et al., 1991)

(Lin and Wolfner, 1989, Thierry-Mieg, 1982, Judd et al., 1972)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Goldberg et al., 1989)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

(Montgomery et al., 1991)

Gene not duplicated in

(Kramers et al., 1991)

(Eeken et al., 1985)

Dp(1;3)N^264-58

(Judd et al., 1972)

(Judd et al., 1972)

(Schalet, 1986)

(Judd et al., 1972)

Gene partially disrupted in

Df(1)Pi4KIIIα^Δ123

(Tan et al., 2014)

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | germline clone

Pi4KIIIα¹³

lethal, with Scer\GAL4^Act5C.PU

Pi4KIIIα^GL00144

lethal (with Pi4KIIIα²¹)

Pi4KIIIα^Δ123

lethal (with Pi4KIIIα^Δ123)

Pi4KIIIα²¹

lethal | recessive

lethal - all die before end of larval stage

Pi4KIIIα⁸⁴

lethal - all die before end of larval stage, with Scer\GAL4^elav-C155

Pi4KIIIα^GL00144

lethal - all die before end of larval stage, with Scer\GAL4^Toll-6-D42

Pi4KIIIα^GL00144

lethal - all die before end of larval stage | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^Mef2.PU

Pi4KIIIα^GL00144

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Pi4KIIIα^GD6917

lethal - all die during first instar larval stage | recessive

Pi4KIIIα^Δ123

partially lethal - majority die

Pi4KIIIα¹⁰

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pi4KIIIα^GD6917

viable, with Scer\GAL4^Mef2.PR

Pi4KIIIα^GD6917

viable, with Scer\GAL4^tin.CΔ4

Pi4KIIIα^GD6917

Other Phenotypes

Allele

abnormal cell polarity | germline clone

Pi4KIIIα^Δ123

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^elav-C155

Pi4KIIIα^GL00144

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Toll-6-D42

Pi4KIIIα^GL00144

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Pi4KIIIα^GL00144

abnormal size | adult stage, with Scer\GAL4^eya.enh1+E+2

Pi4KIIIα^HMS01686

decreased cell number | larval stage | somatic clone

Pi4KIIIα^C

decreased occurrence of cell division | larval stage | somatic clone

Pi4KIIIα^C

increased cell number | larval stage | somatic clone

Pi4KIIIα^C

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Pi4KIIIα^GD6917

some die during larval stage

some die during larval stage | recessive

some die during pupal stage

Pi4KIIIα¹⁰

some die during pupal stage, with Scer\GAL4^pnr-MD237

Pi4KIIIα^GD6917

Pi4KIIIα¹⁰

visible | adult stage, with Scer\GAL4^eya.enh1+E+2

visible | adult stage, with Scer\GAL4^pnr-MD237

Pi4KIIIα^GD6917

visible | cell non-autonomous | somatic clone

Pi4KIIIα¹⁰

Phenotype manifest in

Allele

dorsal appendage | germline clone

Pi4KIIIα^Δ123

egg | germline clone

Pi4KIIIα¹³

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^elav-C155

Pi4KIIIα^GL00144

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^nSyb.PS

Pi4KIIIα^GD6917

Pi4KIIIα¹⁰

eye, with Scer\GAL4^eya.enh1+E+2

eye | cell non-autonomous | male | somatic clone

Pi4KIIIα¹⁰

Pi4KIIIα¹⁰

female reproductive system

Pi4KIIIα¹³

follicle cell | germline clone

Pi4KIIIα^Δ123

follicle cell | somatic clone

Pi4KIIIα^GS27

larval brain | larval stage | somatic clone

Pi4KIIIα^C

mesonotum, with Scer\GAL4^pnr-MD237

Pi4KIIIα^GD6917

neuromuscular junction, with Scer\GAL4^elav-C155

Pi4KIIIα^GD6917

NMJ bouton | larval stage, with Scer\GAL4^elav-C155

Pi4KIIIα^GL00144

nurse cell | germline clone

Pi4KIIIα^Δ123

oocyte nucleus | somatic clone

Pi4KIIIα^GS27

plasma membrane | germline clone

Pi4KIIIα^Δ123

ring canal | germline clone

Pi4KIIIα^Δ123

synapse, with Scer\GAL4^elav-C155

Pi4KIIIα^GD6917

type II neuroblast | larval stage | somatic clone

Pi4KIIIα^C

type I neuroblast | larval stage | somatic clone

Pi4KIIIα^C

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

14 of 14

Yes

Yes

2

3 of 14

No

Yes

3 of 14

No

No

2

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

2

1 of 14

No

No

1

Hsap\BOLA2-SMG1P6

1 of 14

No

Yes

1 of 14

No

No

1

1 of 14

No

Yes

1 of 14

No

No

1

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

13 of 14

Yes

Yes

3 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Mus musculus (laboratory mouse) (17)

14 of 14

Yes

Yes

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

Xenopus tropicalis (Western clawed frog) (6)

8 of 13

Yes

Yes

2 of 13

No

No

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (15)

14 of 14

Yes

Yes

8 of 14

No

Yes

3 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

2 of 14

No

No

Drer\si:rp71-17i16.5

2 of 14

No

No

Drer\zgc:158659

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (5)

14 of 14

Yes

Yes

3 of 14

No

No

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP010801

12 of 12

Yes

Yes

Agam\AgaP_AGAP010273

3 of 12

No

No

Agam\AgaP_AGAP000018

2 of 12

No

No

Agam\AgaP_AGAP007512

2 of 12

No

No

Agam\AgaP_AGAP011036

2 of 12

No

No

Agam\AgaP_AGAP000368

1 of 12

No

No

Agam\AgaP_AGAP003967

1 of 12

No

No

Agam\AgaP_AGAP005533

1 of 12

No

No

Agam\AgaP_AGAP007873

1 of 12

No

No

Agam\AgaP_AGAP010313

1 of 12

No

No

Agam\AgaP_AGAP012631

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (9)

Atha\ATPI4K ALPHA

13 of 13

Yes

Yes

7 of 13

No

Yes

Atha\PI-4KBETA1

3 of 13

No

No

Atha\PI-4KBETA2

3 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Saccharomyces cerevisiae (Brewer's yeast) (7)

11 of 13

Yes

Yes

2 of 13

No

No

2 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Schizosaccharomyces pombe (Fission yeast) (8)

10 of 12

Yes

Yes

2 of 12

No

No

2 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pi4KIIIα. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (8)

5 of 13

5 of 13

4 of 13

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PI4KA; phosphatidylinositol 4-kinase alpha

model of hereditary spastic paraplegia 84

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Arctic.UAS.Tag:SS(nec)}

(Zhang et al., 2017)

Pi4KIIIα^RNAi.UAS.cUa

ameliorates amyotrophic lateral sclerosis

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Forrest et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PI4KA; phosphatidylinositol 4-kinase alpha

14 of 14

hereditary spastic paraplegia 84

PI4KAP2; phosphatidylinositol 4-kinase alpha pseudogene 2

3 of 14

PI4KB; phosphatidylinositol 4-kinase beta

3 of 14

DEAFNESS, AUTOSOMAL DOMINANT 87; DFNA87

autosomal dominant nonsyndromic deafness 87

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Pi4KIIIα - Hyccin

anti bait coimmunoprecipitation, western blot

(Zhang et al., 2022)

Pi4KIIIα - Rab19

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Pi4KIIIα - Rab30

pull down, peptide massfingerprinting

(Gillingham et al., 2014)

Pi4KIIIα - stmA

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, Identification by mass spectrometry

(Zhang et al., 2022, Zhang et al., 2017)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Medina-Yáñez et al., 2020)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Forrest et al., 2013)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0267350

MIST (genetic) - An integrated Molecular Interaction Database

31247

MIST (protein-protein) - An integrated Molecular Interaction Database

31247

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

protein_coding_gene

(Tan et al., 2008)

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-1

Cytogenetic map

Sequence location

X:2,624,804..2,633,672 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3A8-3A8

Limits computationally determined from genome sequence between P{EP}egh^EP804 and P{EP}sgg^EP1576

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-1

(FlyBase, 2016)

1-1.2

(Comeron et al., 2012)

1-1.3

Left of (cM)

sgg

(Judd et al., 1972)

Right of (cM)

Zw10

(Judd et al., 1972)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

3904

Pi4KIIIα²¹/FM7a/Dp(1;2;Y)w⁺

92488

y¹ v¹; P{U6.2-Pi4KIIIα.dgRNA}attP40/CyO

51339

y¹ w^* Pi4KIIIα^FQ88 P{neoFRT}19A/FM7c

51340

y¹ w^* Pi4KIIIα^GS27 P{neoFRT}19A/FM7c

35643

y¹ v¹; P{TRiP.GLV21007}attP2/TM3, Sb¹

35256

y¹ sc^* v¹ sev²¹; P{TRiP.GL00144}attP2

38242

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01686}attP40

10260Rb-1

P{NIG.10260Rb}1

GL00144

y¹ sc^* v¹ sev²¹; P{TRiP.GL00144}attP2

v105614

P{KK100549}VIE-260B

v15993

w¹¹¹⁸; P{GD6917}v15993

v341267

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00318}attP40/CyO-GFP

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (34)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD12145

Other clones

MIP12368

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0267350

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Zhang et al., 2017)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Cell viable, but gynandromorphs show reduced survival.

Relationship to Other Genes

Source for database merge of

Source for merge of: CG10260 Pi4KIIIα

(Brill, 2010.5.19)

Source for merge of: EG:BACR7C10.2 CG10260

(FlyBase, 1996-)

Source for merge of: PI4KIIIα l(1)3Ah

(Tan et al., 2014)

Additional comments

Source for merge of EG:BACR7C10.2 CG10260 was sequence comparison ( date:000425 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: PI4KIIIalpha CG10260

(Yan et al., 2011)

Source for identity of: Pi4KIIIα PI4KIIIα

(FlyBase, 2009-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

CG10260

(Hirschhäuser et al., 2021, Trivedi et al., 2020, Liu et al., 2018, Cockcroft and Raghu, 2016, Balakrishnan et al., 2015, Hosono et al., 2015, Christis and Munro, 2012, Japanese National Institute of Genetics, 2012.5.21, Ni et al., 2011.7.19, Yan et al., 2011, Akimana et al., 2010, Brill, 2010.5.19, Ni et al., 2010.12.1, Wasbrough et al., 2010, Keleman et al., 2009.8.5, Kemppainen et al., 2009)

EG:BACR7C10.2

(Benos et al., 2001, Benos, 2000.2.6)

PI4K

(Jiang et al., 2019, Khuong et al., 2010, Morrison et al., 2000)

PI4KIII_α

(Khuong et al., 2010)

PI4KIIIA

(Cockcroft and Raghu, 2016)

PI4KIIIalpha

(Gene Disruption Project members, 2018-, Lee et al., 2018, Haelterman, 2015.3.20, Forrest et al., 2013, Kwon et al., 2013, Yamamoto et al., 2013-, Rodriguez et al., 2012, Yan et al., 2011, Brill, 2010.5.19)

PI4KinaseIIIα

(Khuong et al., 2010)

Pi4KIIIα

(Zheng et al., 2024, Liu et al., 2023, Cassella and Ephrussi, 2022, Deng and Wang, 2022, Ding et al., 2022, Hu et al., 2022, Wang et al., 2022, Ostalé et al., 2021, Jeon et al., 2020)

Stt4

(Jiang et al., 2016)

ZW-2

(Paradi et al., 1983)

l(1)17-59

l(1)3Ah

l(1)VA722

l(1)zw2

(Williams et al., 1992, Lin and Wolfner, 1989, Garcia-Bellido and Robbins, 1983, Paradi et al., 1983, Lim and Snyder, 1974, Shannon et al., 1972)

stt4

(Yavari et al., 2010)

zw-2

(Kramers et al., 1991, Kramers et al., 1983, Liu and Lim, 1975)

zw2

(Tan et al., 2014, Montgomery et al., 1991, Bourouis et al., 1990, Pastink et al., 1990, Goldberg et al., 1989, Perrimon and Smouse, 1989, Lefevre and Watkins, 1986, Schalet, 1986, Eeken et al., 1985, Robbins, 1983, Thierry-Mieg, 1982, Lefevre, 1981, Judd et al., 1972)

Name Synonyms

PI4KIIIα

Phosphatidylinositol 4-kinase III alpha

Phosphatidylinositol 4-kinase III α

(Graves et al., 2020)

lethal (1) 3Ah

phosphatidylinositol 4-kinase IIIα

(Tan et al., 2014)

zeste-white 2

(Tan et al., 2014)

Secondary FlyBase IDs

FBgn0261922
FBgn0040335
FBgn0029633
FBgn0259120
FBgn0001406

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31247

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9PDM4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9PDM4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31247/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0267350

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0267350

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10260

MARRVEL_MODEL - MARRVEL (model organism gene)

31247

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0267350

DroID - A comprehensive database of gene and protein interactions.

FBgn0267350

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

PI4KIIIalpha_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040335

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0267350

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0267350

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014935

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (genetic) - An integrated Molecular Interaction Database

31247

MIST (protein-protein) - An integrated Molecular Interaction Database

31247

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (125)