FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rab21, Pi3K68D
FlyBase ID
FBig0000095715
Interaction Network
Interactions Browser links
Rab21 network
Pi3K68D network
Reported Interactions
FBrf0218866-17.coIP.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Pi3K68D 

Pi3K68D

bait

FLAG tag

Rab21 

Rab21

prey

GFP tag

Rab21 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab21 
region
mutation increasing interaction
aa 27

T27N mutation mimics inactive GDP-bound state

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)

Pi3K68D binds the inactive form of Rab21 more strongly.

(Jean et al., 2012)
FBrf0218866-18.coIP.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Rab21 

Rab21

bait

FLAG tag

Rab21 
Pi3K68D 

Pi3K68D

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab21 
region
mutation increasing interaction
aa 27

T27N mutation mimics inactive GDP-bound state

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)

Pi3K68D binds the inactive form of Rab21 more strongly.

(Jean et al., 2012)
FBrf0218866-19.coIP.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Rab21 

Rab21

bait

FLAG tag

Rab21 
Pi3K68D 

Pi3K68D

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab21 
region
mutation increasing interaction
aa 27

T27N mutation mimics inactive GDP-bound state

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)

Pi3K68D binds the inactive form of Rab21 more strongly.

(Jean et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)