FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rim, Fife
FlyBase ID
FBig0000108541
Interaction Network
Interactions Browser links
Rim network
Fife network
Reported Interactions
FBrf0234377-1.coIP.WB
Reference
Bruckner et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Fife 

Fife

bait

FLAG tag

Fife 
Fife 
Rim 

RIM

prey

HA tag

Rim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fife 
C2A domain
sufficient binding region

coordinates relative to Fife-PA

Fife 
C2B domain
sufficient binding region

coordinates relative to Fife-PA

Rim 
C2B domain
sufficient binding region

coordinates relative to Rim-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bruckner et al., 2017)

Both C2 domains of Fife bind the Rim C2B domain (but not the Rim C2A domain).

(Bruckner et al., 2017)
FBrf0234377-2.coIP.WB
Reference
Bruckner et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Rim 

RIM

bait

HA tag

Rim 
Fife 

Fife

prey

FLAG tag

Fife 
Fife 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Fife 
C2A domain
sufficient binding region

coordinates not reported

Fife 
C2B domain
sufficient binding region

coordinates not reported

Rim 
C2B domain
sufficient binding region

coordinates not reported

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Bruckner et al., 2017)

Both C2 domains of Fife bind the Rim C2B domain (but not the Rim C2A domain).

(Bruckner et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)