The neurotransmission across neuromuscular junctions in third instar larvae, assessed by excitatory junction potential amplitude and quantal content, is significantly impaired in Fife^AC homozygotes or Fife^AC/Df(3L)Exel6091 transheterozygotes, but not in Fife^AC heterozygotes, as compared to controls.

The neuromuscular junctions of Fife^AC/Df(3L)Exel6091 transheterozygous third instar larvae present a significant decrease in the estimated mean readily releasable vesicle pool and an abnormal synaptic probability of release, as shown by their significantly higher facilitation in paired-pulse experiments, as compared to controls; these neuromuscular junctions also present defective active zones, as shown by a significant decrease in the mean number of membrane-associated synaptic vesicles in their vicinity, significant decreases in the width and complexity of their cytomatrix, and synaptic vesicles with similar numbers and similar mean distance from the membrane, but significantly less frequently docked to the membrane and significantly less frequently associated with the electron-dense cytomatrix, as compared to controls.

The neurotransmission across neuromuscular junctions of Fife^AC/Fife^ex1027 transheterozygous third instar larvae occurs with similar spontaneous miniature excitatory junction potentials, but decreased excitatory junction potentials and decreased quantal content, as compared to controls.

Fife^AC, Rim[+]/Rim¹⁰³ has abnormal neurophysiology | third instar larval stage phenotype

Fife^AC, Rim[+]/Rim¹⁰³ has embryonic/larval neuromuscular junction | third instar larval stage phenotype