AGCAGGATCGTGGCAATGAGGAGATTGTAGCATCTGTGATGACGCACATCGAGAGCCGAGCGTCCAATGGCGCCGGCGTG AGTGTCAACTACAGCGAGGTCACACATTCTCTCCTCTCTGGAGGGCTCAAGAATCGCTTTCTTCCACCTATACCCCCAAA ACCACCCAAGCGTGGCATTCTGAAAGGCTCCCGCAGTAATATGACCAATGTCCACGAGGAGATTAGCTTTGCGGGTAGTG CCGGAGGAGCAGGTGGCACGCCAGAGATGCTATTGCGCAACACCTTTCAGAATGAATCCCTTTACGAGGGAGCTGCCAGG GGAGCTGGTTCGATCGGCTCGAACTCCAGTCAGCATGGAACCTCGTTCACTTCCGTGGAGAGCCTGCGCAGCGATGGAGA GCAGACAGGAGGTCAACAACGGGCCATGACCCTGCCGGCCAACGCCCGCTATGGAGCTTTACCCCAGGGCAACAGTCAAG GATCGCACCTCCATGTGCTCACCTCGCCGTCGCCCAGCGCCGACAGTCTCACAGATACCACAAACTCCTCTTTCGCCACA CCCCCATTTTCGCTGAGTCCCGTCGGCGAGTCCCAAGGCATCGACCGTTGGGCCCGCGTCCATGCCTTCGAGGATGTGGA GCTGCCGCTGCCGCCAGTGCAGCTAGTAAAACTTCCCCCACCAAGGCAGCTAGTGATCAGGCGACAGAAGTCTCCGCGCC AGGACTTCGGCTTTAGCCTGCGCAAGGCCATCTGTTTGGACAGAACAGAGTCCCTGACTTCGCCCATTTTCAGGCCGGTT ATATTCGCCGAGCCAGGTGCTGGTGGCGGAGCCACAGGTCTTTTGCCTGGCGATCGCCTAATCAAGGTCAACGGCACGCC GGTTGGGGAACTGCCGCGGGAGATCATAATTGAGATGATACGGAACAGCGGAGAGGCTGTCACCGTGGAGGTCCAACCAG TCGCTGAGTTGGTGGAGCTGAGCAAGCGATGCATGGCCCCCAGCACAGCCACCGTGGAGGAGATCGATCATTCCATTACC AACGGCAACTGCAATACCCTGCGACGCAGTGCCAGCAAGCGCTTCAAGCGTCAGAGCCGCCATGAGAATGGCAATGGCGG AGGAGAAGAGGGAGGAGCCAAAGGAGTGGGCCACGATCTGGAGGCGGATGTTGCGGATGCCAGTCAGCCGGAGCGCGTAT GGCTGGTGCACCGCGGTGGTTTCACTGCCGCCATCCGTCTGCCGACTGCGTCTTCTGGCCGGGATGAGGAGAACAAGCTA AGCCTGCGCCTCCTGCATAATGGGGAGCAGCTGACCGTCGACGAGGACGATGTCGAGAAGCAGAACAGCCCCGCCCTGGA CCTTGTCGAGGACATCTGCGAGCTGAAGTATCTGAACGAGGCGTCCGTGCTCCATTGCCTGCGTCAGCGATACGCCAGCA ATCTCATCCACACCAAGGCGGGGCCCACGCTGCTCGTGGTCAATCCCATGGCACCGCTGTCCCTGTACTCCGAGAAGGTT GTCTCCATGTTCCGCGGCTGCAAGACGGAGGATATGCCGCCCCACGTCTATTCACTGGCCCAGACCGCCTACAGGAGCCT GGTCGAGACGCGGCGCGACCAGAGCTTGATATTCATGGGCCGATCTGGTTCTGGCAAATCCACTAGCTTCAAGCATGCAC TCAACTACCTGGCGTTGGCAGCTGGTGCCTATAATAATTTTATAAACGCGGAGAAGGTCAATGCCCTGTGCACCATTCTG GAGGCTTTTGGAAATACGAAAACTTGCCTAAACTCGAATGCCACGCGGATGACGCAGCTGTTAAGCCTGGATTTCGATCA AACCGGCCAGATTGCATCTGCCTCATTGCAAGTTCTCCTCCCAGAAAGACAACGAGCTGGCCGACGACTTGGTCATGAAC ATAGCTTCCACATCATGACGAGACTTTTGGCTGGCGCTGCTGGCTTGCTACAGAAGGAGCTGCACCTGGAGAACATAACC TCTGAGGACAGCCATCCTTTTATATCGTTATCCCAAAAGCTGGAAGATCGGCATCGAGCTGCCAATGACTTTATGCGCAC TGTTCAGGCCTTTGAGACGCTGAACATTGATGCGAAGGCGGTACGTGGGATCTGGAGCATTTTGGCGGCCATTTACCATC TGGGCATTGCTGGCGTCACAAAACTGGGCACTGGCTCCACAGCCCGAACTCAGTTTGCCAATCCCACGGCCGCCCGAAAG GCCTCGGGCTTGTTGGGCGTGAATCTGGAGGATCTGTCATCAGCTGCCTTTGGTCTTACCCAACCAAACGCCCCCAATGG CGGTCTGAGTCCATCGAAATCGCCCACCTCGGACACTGGACACGAATGGGCCTGGGAATGTCTCGAGGCGTTGGTCATTG GTCTATATTCGGAGGCCTTGGCTGCAGTGGTGGCATTGATCAATCGTCAGATCTGCACATCGTCCCACACCATAGCCTCA ATTATGCTAATTGACACGCCCGGCTTCCAAAATCCAGCCAGCTGTGGCCAGCAAGTGGGTGCTACTCTAGCGGATCTGCG TCACAACTATCTACAGGAGCGTCTACAGATGCTCTTCCATCACACCACACTGGTGGCTCCCCGCGATCGCTACGCCCAGG AGCTGGTAGAAATCGAGATGGACCTGGCATCGGAGTGTCATCCGGGACCGTTGATCTCGCTTATCGACAAGGCACCTCAG AATCATGTAGTGCGCTCGTCCCAGCGAGATTTGCGAGAGCATGATCGTCGAGGAATGTTGTGGCTACTGGATGAGGAAGC TATCTACCCCAACTCCAACGATGACACATTCCTTGAACGTTTGTTCTCCCACTACGGTGACCGGGAACATCATAGCCTGC TGCGCAAATGTGCAGGTCCGCGACAATTTGTACTCCACCATCTGCAAGGCACCAATCCCGTTCTCTACGCTGTCGATGGA TGGGTGCGTCATAGCCGGGAGCACCCAGGTATTCGGAATGCTGTATCCCTACTACAGGACAGCAGTCGGGAGGAGATCAA TCGCCTATATATCGGCTCACTGACTCGAGGATCGGGAGCAATGGTGTTTTGTGGCTCTTTTGCTGGACTGGAAGGCACTC AGTCACTTCGTCGCGTGTCCAGTATTCGCCGCTCCTTCACAACGGCCGGAGTAAAGCGAAACTCCATAATGCTGCAGGTG AAGTTCACAGTGGATGGAATTATCGACACTTTGCGCCGCACTGGAACTCACTTTGTGCACTGCTATCTGCTGCAACATAA CGCAGGCAAACATACCAAGTACACCGCCAATGGATCGCCCAGCTCAGCTGCCGGTCAGGTGTCCAGCGAGGAGGAAATGG TCAACGTGCCACTGTTAAGGAGTCAGCTACGTGGTTCACAAGTCCTAGAAGCGGCTCGCTTGCATCGCCTTGGTTTTCCC GAATCAGTGCCATTGTTGGAATTTGTCAGGCGTTTCGGGCTTTTGGCCGGGGATTTGGCTAGCAATAAGGATGTGAGCGT GGAGCAGATATTAGCTGTCAATGAACTGGATGTGGCCAGCTATCGCATAGGACCCAGCCAGATCCTCTTCCGCTCAGGAG TTCTTAGTGAACTGGAAGCCAAGCGCGATGTCCTGCTCTCAGATCGCATTATACAGCTGCAAGCATTTTGCCGCGGCTAC TTGGCACGCAAGAAGATGTCCCAACGAAGGGTCCAGGAACTGGCAGTGCGTTGCATTCAACGCAATGTGAAGGCATTCCT TGCCGTTCGCGACTGGCCGTGGTGGCGTCTCCTGGTGCGGGTCACTCCCCTGCTCAACGTTCATCGCACCGAGGAGCAGC TGAAGACGGCTAACGAGGAGCTCCTAATGCTGCGAGCCAAGCTGGAGAAGATCGAGTGTGATCGCAGTGAGGTCAAGGCA GAAAACCAAAAGCTGGAAGCCAAGCTATCCGAGCTGACGGTGGACCTGGCCGAGGAGCGATCCACGGCTCATATAGCTAC CGAGCGGCTGGAGGCGGAAACCGCCGAACGCTTGAAGCTGGAGAAGGAGCTGGGCGATCAAACCAACAAGGTGAAGAACC TTCAGGAGACGACGGAGAAGCTGGAAATGGAGCTGATATGCGCCAAGTCCGATCTGAATGGCATCTCTGAGGACGAGGAT GCAGAGAACGAGGATGGGGTCGGCGGCGGCGTCTACAAGCTTAAGTACGAGCGGGTGGCCAGGGAGCTGGAGTTCACCAA AAGGCGGTTACACACGCAGCATGAGCACGATCTGGAACAGCTGGTCGCGCTCAAGAAGCATTTGGAGATGAAGCTTTCCG ATGCCTACGAAGAAGTTGTGGAACAACGTCAGGTTGTGGGCCAATGGAAGCGCAAGGCACAGAAGATGACCAACGAGATG AACGATCTGCGCATGCTGCTCGAGGAGCAAAATGCGCGCAACAATTTGCTCGAGAAGAAGCAGCGCAAGTTCGACGCCGA GTGCCAGTCCCTGCAGGATGCAGTGCGTCAGGAGCGACAGGCCAAAGAGCGCTACGGTCGCGAAAAGGACGTCCTGCAGG CCGAGAAGTTCACACTGGAGCAAACGCTGGCGGACACCCGTCTGGATCTTGAATTCAAGGAGGAGAAACTTGCATCGCTA CAGCGCGAGCTGGAGGAGATGACCTTTGGTGGCGGCACCGAGGAGGAGTTCGCCCAACTGCGGCGCTCCAAAAATGAAAC AGAGCGTCGGGCCAAGGAACAGGAGGAAGAACTGGACGAGATGGCCGGTCAGATACAGCTGCTCGAACAAGCCAAGCTTC GCCTGGAGATGACCCTGGAAACAATGCGCAAAGAAGCGCGCCGTGAGTCCCAGCAGCGCGACGAAGAGCTGGAAGAAGTA CGCGGTAACGGCTACAAGAAGATTAAAGCCCTTGAATGCCAGCTGGAAACTGAGCACGAGGAGCGGACTCTGCTGCTGCG CGAGAAGCACGAGCTAGAGCGACGTCTCTCCTCCATGGAGGATCGCGATCGTGTTGATCGCGATGCCGAGGAAGCGCTCA ACCAAAAACTGCGACGCGATCTTCGCAAATACAAGGCCCTGCTCAAGGACGCCCAGACACAGTTGGAGCGGCTTAAGGCT GACACGCCTGGCAAGACGCTTATAAGACAACTGCGTAATCAACTGGAGGATGCCGAATCTGCTCGTTCGCTGGCTATGAA AGCGCGACAAACTGCGGAAGCCGAACTTACTGAAGTGCAGGCCATGTTCGATGAGTCGCATCGAGCTAGGAATGACGCGG AGGAGCGAGCCAATGCCGCACACAGAGATCGGGCTGAGCTGCAGGCCCAGATCGAGGAGAACGAGGAGGAGCTGGGCGAG CTGATGAAGAAGTACAGCGCCACGGTAAAGCAACTAAATACAGAGCAAATCAACGTATCCGAGGCTGAGTTTAAACTCAA TGAAATGGAAGCAGAGCGCAATAACCTCAAGGAGCAGGTGGCCGAGCTGCAGCACCGACTGGACAACGTTGAGAACTTGG GGGATCCATCTATGGCCATGATGTCCAAGCGACTGGAGCTGCGCACCAAGGAATTAGAGTCTCGACTGGAACTAGAGCAG GCCACTCGGGCTCGCCTTGAAGTGCAGGTGAACCGTCACAAAGAGGCTCTGGAAAAACTGCAGAACGAGGTGACGCAGTC AAAGATGCGTGAGATGCAGGCCCAGGATGTAATCAAAAAATCGCAAAAGAGTCTGCGCGACATGCGCGAAGAGTTCCACG CTGTCTCCAGCCGCGAGCAGGAGTCGCTCACAAGGCGCAAGGACCTGGAGAAGAAGGTGGAGCAAATGGAGTCGGAGGGA GCGGCGCTTAAAAACGATCTACGACTGGCTTTACAGCGAATAGCTGATCTACAGCAGGCAATGGAGGAAGAGGGCGAGGA GGAGCTGAGCGAAAGTGATGAAAGCCTCAGTTCGGTGGGCTCTATCAGTGATCTGGAAGATCGACTTCGGCCAGTTCATG TAAAACGCAGCTCACAGCAGTCCTTGAACGGCAGCATCGGCGGCGGAGGCGGAAGCGTTGTCAGCTCCACCCGCACCGTG GTGTTCGAAAAGGACGACAACAGCCCGAGAAATAACGCCAAGTAACGGGAAAGATTCATAAAAACCTTTATAAAAACTAA CAAAACGAATATAAACAAATAAATATATTGTTATAAAACTTAAAAAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
