Males expressing Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Abd-B-LDN show no rotation of the genitalia in 40% of cases, and partial sinistral male genitalia rotation (instead of the wild-type dextral rotation) in 60% of cases.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Abd-B-LDN results in an apparent genitalia "non-rotation phenotype" in males (0[o], no spermiduct looping an genitalia correctly oriented). However, time-lapse imaging reveals that both of the rings in the genital disc which normally rotate do spin, although in opposite directions: the A8a domain rotates counterclockwise by 180[o] (-180[o]) whereas the A8p domain rotates clockwise by 180[o] (+180[o], real movement).

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^hh-Gal4 results in an apparent genitalia "non-rotation phenotype" in males. Time-lapse imaging reveals that both of the rings in the genital disc which normally rotate do spin, although in opposite directions: the A8a domain rotates clockwise by 180[o] (+180[o]), while the A8p domain rotates anti-clockwise by 180[o] (-180[o], relative or real movement).

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^byn-Gal4 results in an inversion of the asymmetry of the adult, but not the embryonic, hindgut.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^byn-Gal4 results in an inversion of the asymmetry of the adult, but not the embryonic, hindgut.

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^cad-md509 has no affect on the rotation of the male genitalia.

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of either Scer\GAL4^en-e16E or Scer\GAL4^hh-Gal4 results in a striking non-rotated male genitalia phenotype.

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^Abd-B-LDN results in 69% of males showing partial sinistral rotation of the genitalia, instead of the normal dextral rotation (30% of these males show no rotation of the genitalia).

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^dpp.blk1 results in 32% of males showing only partial dextral rotation of the genitalia, instead of the wild-type complete dextral rotation.

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^tsh-md621 results in males showing partial or complete sinistral rotation of the genitalia.

Expression of P{Sym-UAS-Myo31DF} (which carries both Myo31DF^Sym.Scer\UAS and Myo31DF^{a.Sym.Scer\UAS}) under the control of Scer\GAL4^Abd-B-LDN in the presence of Scer\GAL80^ts.αTub84B does not affect male genitalia rotation at 25^oC (the permissive temperature for Scer\GAL80^ts.αTub84B), but results in a non-rotated male genitalia phenotype at 30^oC (the restrictive temperature for Scer\GAL80^ts.αTub84B). Single temperature shifts using these flies indicate that Myo31DF function is required at day 6 of development.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^cad-md509 has no affect on the rotation of the male genitalia.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of either Scer\GAL4^en-e16E or Scer\GAL4^hh-Gal4 results in a striking non-rotated male genitalia phenotype.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Abd-B-LDN results in 69% of males showing partial sinistral rotation of the genitalia, instead of the normal dextral rotation (30% of these males show no rotation of the genitalia).

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 results in 32% of males showing only partial dextral rotation of the genitalia, instead of the wild-type complete dextral rotation.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of either Scer\GAL4^ptc-559.1 or Scer\GAL4^tsh-md621 results in males showing partial or complete sinistral rotation of the genitalia.

Expression of Myo31DF^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^Abd-B-LDN in the presence of Scer\GAL80^ts.αTub84B does not affect male genitalia rotation at 25[o]C (the permissive temperature for Scer\GAL80^ts.αTub84B), but results in a non-rotated male genitalia phenotype at 30[o]C (the restrictive temperature for Scer\GAL80^ts.αTub84B). Single temperature shifts using these flies indicate that Myo31DF function is required at day 6 of development.