FlyBase Allele Report: Dmel\Arp2[GD14716]

General Information

Symbol

Dmel\Arp2^GD14716

Species

D. melanogaster

Name

FlyBase ID

FBal0198757

Feature type

allele

Associated gene

Dmel\Arp2

Associated Insertion(s)

Carried in Construct

P{GD14716}

Also Known As

UAS-Arp2 RNAi, arp2 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14716}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14716

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^C57

(Nahm et al., 2010)

abnormal neuroanatomy | larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

abnormal neuroanatomy | larval stage, with Scer\GAL4^VGlut1-OK371

(Koch et al., 2014)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

abnormal wound healing | P-stage, with Scer\GAL4^A58

(Rötte et al., 2024)

lethal | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

bouton, with Scer\GAL4^C57

(Nahm et al., 2010)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

lamellipodium | P-stage, with Scer\GAL4^A58

(Rötte et al., 2024)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

neuromuscular junction, with Scer\GAL4^C57

(Nahm et al., 2010)

synapse, with Scer\GAL4^C57

(Nahm et al., 2010)

tormogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell | increased number, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

type Ib terminal bouton | larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

type Ib terminal bouton | larval stage, with Scer\GAL4^VGlut1-OK371

(Koch et al., 2014)

Detailed Description

Statement

Reference

Larvae expressing Arp2^GD14716 under the control of Scer\GAL4^Ubi have neuromuscular junction (NMJ) defects. The number of type Ib boutons per NMJ and the number of axonal branch points per NMJ are significantly reduced compared to controls.

Larvae expressing Arp2^GD14716 under the control of Scer\GAL4^VGlut-OK371 show a significant reduction in the number of type Ib boutons per NMJ compared to controls, but the number of axonal branch points per NMJ is not significantly affected.

The average speed of migration of third instar larvae expressing Arp2^GD14716 under the control of Scer\GAL4^Ubi is significantly reduced compared to wild type.

(Koch et al., 2014)

Muscle-specific expression of Arp14D^GD14716 under the control of Scer\GAL4^C57 results in synaptic overgrowth.

(Nahm et al., 2010)

Adults expressing Arp2^GD14716 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing Arp2^GD14716 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in duplication of bristle hairs on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 10-20% of the Scer\GAL4^pnr-MD237 expression domain of the notum.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 70-80% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development