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General Information
Symbol
Dmel\Myo31DFUAS.GFP
Species
D. melanogaster
Name
FlyBase ID
FBal0194495
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Myo31DF-GFP
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the full length Myo31DF coding region tagged with GFP.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Males expressing Myo31DFScer\UAS.T:Avic\GFP under the control of Scer\GAL4Myo31DF-NP1548 show normal male genital rotation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of Myo31DFScer\UAS.T:Avic\GFP suppresses the defects in male genital rotation caused by expression of Myo61FScer\UAS.T:Disc\RFP under the control of Scer\GAL4Myo31DF-NP1548, such that all males show normal dextral rotation of the genitalia.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of Myo31DFScer\UAS.T:Avic\GFP under the control of Scer\GAL4ptc-559.1 completely rescues the change in direction of male genitalia rotation seen in Myo31DFK2/Myo31DFL152 mutant pupae. Mutants expressing Myo31DFScer\UAS.T:Avic\GFP undergo the 360[o] dextral (right-handed) rotation rather than the left-handed (sinistral) rotation seen in Myo31DFK2/Myo31DFL152 mutations.

Expression of Myo31DFScer\UAS.T:Avic\GFP in embryos raised at 18[o]C under the control of Scer\GAL4byn-Gal4 and Scer\GAL80ts.αTub84B partially rescues the left-right (LR) inversion of the gut seen in Myo31DFL152 mutants. Approximately 45% of embryos show gut inversion, compared to 40% in the absence of the driver. When embryos undergo a 60 minute temperature shift to 32[o]C at between 10 and 12.75 hours after egg laying effectively rescued the LR defects, The efficiency of rescue increasing up to 12 hours after egg laying but if the temperature shift takes place after 13 hours after egg laying rescue is less effective. The embryos shifted to 32[o]C at 12.75 hours are mostly at the late stage of germband retraction.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Myo31DFScer\UAS.T:Avic\GFP
Myo31DFUAS.GFP
Name Synonyms
Secondary FlyBase IDs
    References (11)