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FB2021_04, released August 17, 2021

Gene: Dmel\DCTN2-p50

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General Information
Symbol
Dmel\DCTN2-p50
Species
D. melanogaster
Name
Dynactin 2, p50 subunit
Annotation Symbol
CG8269
Feature Type
protein_coding_gene
FlyBase ID
FBgn0021825
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Dynactin 2, p50 subunit (DCTN2-p50) encodes a subunit of the dynactin complex. Together with other members of the dynactin complex, the product of DCTN2-p50 is critical for most functions of the minus end-directed microtubule motor cytoplasmic dynein-1, including oocyte polarity, mRNA localization, centrosome localizations, spindle organization, endocytosis and axonal transport. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Dmn, dynamitin, p50, p50-dynamitin, l(2)k16109

Key Links
Genomic Location
Cytogenetic map
44F3-44F3
Sequence location
2R:8,891,832..8,893,339 [+]
Recombination map
2-59
RefSeq locus
NT_033778 REGION:8891832..8893339
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (17 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (11 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in centrosome localization
inferred from mutant phenotype
(Morales-Mulia and Scholey, 2005)
involved_in clathrin-dependent endocytosis involved in vitellogenesis
inferred from direct assay
(Liu et al., 2015)
involved_in mRNA transport
inferred from direct assay
(Amrute-Nayak and Bullock, 2012)
involved_in oogenesis
inferred from direct assay
(Liu et al., 2015)
involved_in phototransduction
inferred from mutant phenotype
(Huang et al., 2015)
involved_in pole plasm oskar mRNA localization
inferred from direct assay
(Liu et al., 2015)
involved_in positive regulation of retrograde axon cargo transport
inferred from mutant phenotype
(Butzlaff et al., 2015)
involved_in retrograde axonal transport
inferred from mutant phenotype
(Yang et al., 2008)
involved_in spindle organization
inferred from mutant phenotype
(Morales-Mulia and Scholey, 2005)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in mitotic metaphase plate congression
inferred from sequence or structural similarity with UniProtKB:Q13561
(assigned by UniProt )
(UniProt Curators, 2002-)
involved_in mitotic spindle organization
inferred from biological aspect of ancestor with PANTHER:PTN000394123
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:Q13561
(assigned by UniProt )
(UniProt Curators, 2002-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
colocalizes_with axon cytoplasm
inferred from direct assay
(Pilling et al., 2006)
located_in cell cortex
inferred from direct assay
(Liu et al., 2015)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
is_active_in centrosome
inferred from biological aspect of ancestor with PANTHER:PTN001034318
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000394123
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of dynactin complex
inferred from sequence or structural similarity with UniProtKB:Q13561
(assigned by UniProt )
(UniProt Curators, 2002-)
inferred from biological aspect of ancestor with PANTHER:PTN000394123
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in vesicle
inferred from sequence or structural similarity with UniProtKB:Q13561
(assigned by UniProt )
(UniProt Curators, 2002-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the dynactin subunit 2 family. (Q7K2D2)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Dynactin 2, p50 subunit (DCTN2-p50) encodes a subunit of the dynactin complex. Together with other members of the dynactin complex, the product of DCTN2-p50 is critical for most functions of the minus end-directed microtubule motor cytoplasmic dynein-1, including oocyte polarity, mRNA localization, centrosome localizations, spindle organization, endocytosis and axonal transport. [Date last reviewed: 2018-10-04]
Gene Group (FlyBase)
DYNACTIN COMPLEX -
Dynactin is a large 1.2 MDa multisubunit protein complex that associates with the cytoplasmic dynein complex to drive microtubule-based transport. (Adapted from PMID:15473859 and PMID:25751425).
Protein Function (UniProtKB)
Modulates cytoplasmic dynein binding to an organelle, and plays a role in prometaphase chromosome alignment and spindle organization during mitosis. May play a role in synapse formation during brain development (By similarity).
(UniProt, Q7K2D2)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\DCTN2-p50 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
DCTN2-p50-RA
FBtr0088641
NM_079951
1358
380
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
DCTN2-p50-PA
FBpp0087722
42.0
380
4.91
NP_524690
AAF59034
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Subunit of dynactin, a multiprotein complex associated with dynein.

(UniProt, Q7K2D2)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\DCTN2-p50 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
spermatogenesis & adult stage | male
spermatogonial cyst
(Wu et al., 2016)
spermatocyte
(Wu et al., 2016)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with axon cytoplasm
inferred from direct assay
(Pilling et al., 2006)
located_in cell cortex
inferred from direct assay
(Liu et al., 2015)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\DCTN2-p50 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of DCTN2-p50
Transgenic constructs containing regulatory region of DCTN2-p50
Aberrations (Deficiencies and Duplications) ( 6 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal
lethal | recessive
lethal - all die before end of pupal stage, with Scer\GAL4pnr-MD237
viable
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
Other Phenotypes
Allele
abnormal neuroanatomy | larval stage
abnormal neurophysiology | adult stage | somatic clone
increased cell death | adult stage | progressive | somatic clone
some die during pupal stage, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
axon, with Scer\GAL4Toll-6-D42
dorsal appendage
egg chamber, with Scer\GAL4VP16.mat.αTub67C
embryonic/larval dorsal branch | embryonic stage, with Scer\GAL4btl.PU
embryonic/larval nervous system, with Scer\GAL4elav-C155
eye | somatic clone
eye photoreceptor cell | somatic clone
ganglion mother cell | larval stage
larval neuroblast | larval stage
microtubule | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
oocyte & nucleus, with Scer\GAL4VP16.mat.αTub67C
oocyte | germline clone
oocyte nucleus | oogenesis stage S9
retina | somatic clone
rhabdomere of eye photoreceptor cell | somatic clone
vesicle | adult stage | female, with Scer\GAL4VP16.mat.αTub67C
yolk, with Scer\GAL4VP16.mat.αTub67C
yolk granule, with Scer\GAL4VP16.mat.αTub67C
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\DCTN2
15 of 15
Yes
Yes
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Dctn2
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Dctn2
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\dctn2
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\dctn2
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\dnc-2
15 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\JNM1
3 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mug1
3 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190B0P )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00006804
Drosophila simulans
Dsim\GD10607
Drosophila sechellia
Dsec\GM21071
Drosophila erecta
Dere\GG23390
Drosophila yakuba
Dyak\GE19232
Drosophila ananassae
Dana\GF12385
Drosophila pseudoobscura pseudoobscura
Dpse\GA20943
Drosophila persimilis
Dper\GL17128
Drosophila willistoni
Dwil\GK20679
Drosophila virilis
Dvir\GJ21298
Drosophila mojavensis
Dmoj\GI18927
Drosophila grimshawi
Dgri\GH23102
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915075R )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013401
Glossina morsitans
Tsetse fly
Gmor\GMOY002966
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11287
Mayetiola destructor
Hessian fly
Mdes\Mdes005639
Mayetiola destructor
Hessian fly
Mdes\Mdes012244
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL011590
Aedes aegypti
Yellow fever mosquito
Aaeg\Dmn
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005875
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W09AA )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009875
Danaus plexippus
Monarch butterfly
Dple\DPOGS200821-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL011477
Apis florea
Little honeybee
Aflo\2352086404
Apis mellifera
Western honey bee
Amel\Dctn2
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25064
Bombus terrestris
Buff-tailed bumblebee
Bter\15204982
Linepithema humile
Argentine ant
Lhum\LH24418
Linepithema humile
Argentine ant
Lhum\LH24402
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001654
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008652
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12539
Tribolium castaneum
Red flour beetle
Tcas\TC001942
Pediculus humanus
Human body louse
Phum\PHUM169880
Rhodnius prolixus
Kissing bug
Rpro\RPRC008709
Cimex lectularius
Bed bug
Clec\CLEC000625
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005408
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI48826
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_00741
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X096L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR007373
Ixodes scapularis
Black-legged tick
Isca\ISCW005007
Stegodyphus mimosarum
African social velvet spider
Smim\KFM76157.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur10g00060
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_300326
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0D0D )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Dynamnp50ph
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000029062
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Evidence
    References
    DCTN2-p50k16109
    model of  retinal degeneration
    CEA
    (Huang et al., 2015)
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    DCTN2-p50GD13758
    exacerbates  tauopathy
    modeled by Hsap\MAPTR406W.UAS
    (Butzlaff et al., 2015)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    DCTN2; dynactin subunit 2
    15 of 15
    607376
         
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        DCTN2-p50 - Arp1
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - Arp10
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - BicD
        pull down, western blot
        (Dix et al., 2013)
        DCTN2-p50 - CG7407
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - DCTN1-p150
        experimental knowledge based, anti tag coimmunoprecipitation, anti tag western blot, western blot
        (Guruharsha et al., 2011, Kim et al., 2007, Basto et al., 2004)
        DCTN2-p50 - DCTN3-p24
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - DCTN4-p62
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - DCTN5-p25
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - DCTN6-p27
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - Dhc64C
        pull down, western blot, anti tag coimmunoprecipitation
        (Dix et al., 2013, Basto et al., 2004)
        DCTN2-p50 - LRR
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - Lis-1
        pull down, western blot
        (Dix et al., 2013)
        DCTN2-p50 - Tbc1d15-17
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - cpa
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - cpb
        experimental knowledge based
        (Guruharsha et al., 2011)
        DCTN2-p50 - mbt
        anti tag coimmunoprecipitation, western blot, enzymatic study, autoradiography
        (Menzel et al., 2007)
        DCTN2-p50 - rod
        anti tag coimmunoprecipitation, western blot
        (Basto et al., 2004)
        DCTN2-p50 - sw
        pull down, western blot
        (Dix et al., 2013)
        RNA-protein
        Physical Interaction
        Assay
        References
        DCTN2-p50 - fs(1)K10
        pull down, western blot, peptide massfingerprinting
        (Dix et al., 2013)
        DCTN2-p50 - h
        pull down, peptide massfingerprinting, western blot
        (Dix et al., 2013)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        DCTN2-p50
        enhanceable
        DCTN1-p150
        (Duncan and Warrior, 2002)
        DCTN2-p50
        enhanceable
        Khc
        (Duncan and Warrior, 2002)
        DCTN2-p50
        enhanceable
        Klc
        (Duncan and Warrior, 2002)
        DCTN2-p50
        suppressible
        Rab5
        (Liu et al., 2015)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Dys
        suppressible
        DCTN2-p50
        (Kucherenko et al., 2008)
        peb
        suppressible
        DCTN2-p50
        (Wilk et al., 2004)
        External Data
        Subunit Structure (UniProtKB)
        Subunit of dynactin, a multiprotein complex associated with dynein.
        (UniProt, Q7K2D2 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-59
        Cytogenetic map
        44F3-44F3
        Sequence location
        2R:8,891,832..8,893,339 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        44F3-44F3
        Limits computationally determined from genome sequence between P{lacW}Rya-r44Fk04913 and P{lacW}Dmnk16109&P{lacW}Gγ1k08017
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        44F3-44F4
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        44F2-44F6
        44F3--4
        (BDGP Project Members, 1994-1999)
        Experimentally Determined Recombination Data
        Location

        2-59

        (FlyBase, 2016)

        2-57.9

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (15)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (26)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: DCTN2-p50 Dmn

          (Hays et al., 2015.6.30)
          Source for database merge of

          Source for merge of: Dmn CG8269

          (Misra, 2000.8.9)
          Additional comments
          Other Comments

          RNAi screen using dsRNA made from templates generated with primers directed against this gene causes spindle pole detachment when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

          (Goshima et al., 2007)

          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

          (Kiger et al., 2003)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 36 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DRscDB
          EMBL-EBI Single Cell Expression Atlas
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL
          modMine - A data warehouse for the modENCODE project
          Linkouts
          ApoDroso - Functional genomic database for photoreceptor development, survival and function
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (22)
          Reported As
          Symbol Synonym
          CG8269
          (Butzlaff et al., 2015, Hosono et al., 2015, Koya and Meller, 2015, Rosa-Ferreira et al., 2015, Keleman et al., 2009.8.5, Perkins et al., 2009, Goshima et al., 2007, Morales-Mulia and Scholey, 2005, Eckley and Schroer, 2003, Goldstein and Gunawardena, 2000)
          DCTN2
          (Kollmar, 2015.9.21, Hammesfahr and Kollmar, 2012)
          DCTN2-p50
          (Zheng et al., 2020, Hays et al., 2015.6.30)
          DMN
          (Sitaram et al., 2012)
          Dmn
          (Chougule et al., 2020, Lv et al., 2019, Yuan et al., 2019, Kline et al., 2017, Caviglia et al., 2016, Wu et al., 2016, Koya and Meller, 2015, Liu et al., 2015, Winkler et al., 2015, Soundararajan and Bullock, 2014, Amrute-Nayak and Bullock, 2012, Japanese National Institute of Genetics, 2012.5.21, Wong et al., 2012, Kucherenko et al., 2011, Neumüller et al., 2011, Higashi-Kovtun et al., 2010, Anderson et al., 2009, Dworkin et al., 2009, Navarro et al., 2009, Nicolas et al., 2009, Gervais et al., 2008, Jaramillo et al., 2008, Kucherenko et al., 2008, Yang et al., 2008, Christensen and Cook, 2007.3.22, Goshima et al., 2007, Weil et al., 2006, Ferree et al., 2005)
          Dynamitin
          (Butzlaff et al., 2015)
          P50
          (Kollmar and Odronitz, 2006, Basto et al., 2004, Basto et al., 2004)
          dDyn
          (Zimyanin et al., 2007, Zimyanin et al., 2007)
          dmn
          (Gáspár et al., 2017, Huang et al., 2015, Reis et al., 2012, Zheng et al., 2008, Mirouse et al., 2006, Ji et al., 2002)
          dyna
          (Higashi-Kovtun et al., 2010)
          l(2)k16109
          (BDGP Project Members, 1994-1999)
          l(2)k16218
          (Dockendorff et al., 2000)
          p50
          (Butzlaff et al., 2015, Reis et al., 2012, Yang et al., 2008, Haghnia et al., 2007)
          p50/Dmn
          (Wojcik et al., 2001)
          p50/dynamitin
          (Welte, 2004, Tekotte and Davis, 2002)
          p50Dmn
          (Dix et al., 2013)
          Name Synonyms
          Dynactin 2, p50 subunit
          Dynactin2 p50
          (Kollmar, 2015.9.15)
          Dynamitin
          (Winkler et al., 2015, Dix et al., 2013, Gervais et al., 2008, Yang et al., 2008, Januschke et al., 2007, Ferree et al., 2005, Spradling et al., 1999)
          dynamitin
          (Sulkowski et al., 2014, Lloyd et al., 2012, Higashi-Kovtun et al., 2010, Nicolas et al., 2009, Zheng et al., 2008, Haghnia et al., 2007, Menzel et al., 2007, Huynh and St. Johnston, 2004, Duncan et al., 2001)
          p50 dynamitin
          (Wainman et al., 2009, Cytrynbaum et al., 2003)
          p50-dynamitin
          (Kim et al., 2007, Morales-Mulia and Scholey, 2005, Wojcik et al., 2000)
          p50/Dynamitin
          (Pilling et al., 2006)
          Secondary FlyBase IDs
          • FBgn0033338
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (125)