FlyBase Allele Report: Dmel\DCTN2-p50[GD13758]

General Information

Symbol

Dmel\DCTN2-p50^GD13758

Species

D. melanogaster

Name

FlyBase ID

FBal0206870

Feature type

allele

Associated gene

Dmel\DCTN2-p50

Associated Insertion(s)

Carried in Construct

P{GD13758}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13758}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13758

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

exacerbates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cIb

(Ueda et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

axon, with Scer\GAL4^Toll-6-D42

(Butzlaff et al., 2015)

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

Detailed Description

Statement

Reference

DCTN2-p50 silencing in segmental nerves of L3 larvae (DCTN2-p50^GD13758, Scer\GAL4^D42) significantly reduces the rate of mitochondria transported in the retrograde direction, but has no effect on retrograde speed or anterograde speed/rate.

DCTN2-p50 silencing (DCTN2-p50^GD13758, Scer\GAL4^elav-C155) does not result in any obvious changes to the microtubule network in larval segmental nerves.

Scer\GAL4^elav-C155-mediated expression of DCTN2-p50^GD13758 has no apparent effect on larval motor coordination or locomotion, or on eclosion rates.

(Butzlaff et al., 2015)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain associated with decreased size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in a reduction in the average size of neuroblasts together with shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing DCTN2-p50^GD13758 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of DCTN2-p50^GD13758 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD13758} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13758

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

DCTN2-p50^GD13758, Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155 has partially lethal phenotype

(Butzlaff et al., 2015)

DCTN2-p50^GD13758, Hsap\MAPT^R406W.UAS, Scer\GAL4^Toll-6-D42 has abnormal locomotor behavior | progressive phenotype

(Butzlaff et al., 2015)

Phenotype Manifest In

Enhancer of

Statement

Reference

DCTN2-p50^GD13758, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Enhancer of

Statement

Reference

DCTN2-p50^GD13758, Scer\GAL4^elav-C155 is a non-enhancer of neuromuscular junction | larval stage phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155

(Butzlaff et al., 2015)

DCTN2-p50^GD13758, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Suppressor of

Statement

Reference

DCTN2-p50^GD13758, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

Other

Statement

Reference

DCTN2-p50^GD13758, Hsap\MAPT^R406W.UAS, Scer\GAL4^elav-C155 has axon | larval stage phenotype

(Butzlaff et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

DCTN2-p50 silencing (DCTN2-p50^GD13758, Scer\GAL4^elav-C155) in combination with Hsap\MAPT^{R406W.Scer\UAS} expression does not result in any obvious changes to the microtubule network in larval segmental nerves.

Scer\GAL4^elav-C155-mediated expression of Hsap\MAPT^{R406W.Scer\UAS} in combination with silencing of DCTN1-p150 (DCTN1-p150^GD1455) or DCTN2-p50 (DCTN2-p50^GD13758) results in axonal swellings in larval segmental nerves.

Scer\GAL4^elav-C155-mediated co-expression of Hsap\MAPT^{R406W.Scer\UAS} and DCTN2-p50^GD13758 has no apparent effect on larval motor coordination or locomotion.

Scer\GAL4^elav-C155-mediated co-expression of Hsap\MAPT^{R406W.Scer\UAS} with DCTN2-p50^GD13758 strongly reduces eclosion rates.

Scer\GAL4^D42-mediated co-expression of Hsap\MAPT^{R406W.Scer\UAS} with DCTN2-p50^GD13758 has no impact on viability but an age-dependent decline in locomotion skills is observed.

(Butzlaff et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v23726

w¹¹¹⁸; P{GD13758}v23726

Notes on Origin

Discoverer