FlyBase Allele Report: Dmel\DCTN2-p50[k16109]

General Information

Symbol

Dmel\DCTN2-p50^k16109

Species

D. melanogaster

Name

FlyBase ID

FBal0064065

Feature type

allele

Associated gene

Dmel\DCTN2-p50

Associated Insertion(s)

P{lacW}DCTN2-p50^k16109

Carried in Construct

Also Known As

dmn^K16109, l(2)k16109

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}DCTN2-p50^k16109

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,891,862..8,891,862 [+]

(Yang et al., 2008, Januschke et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of retinal degeneration

CEA

(Huang et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage | somatic clone

(Huang et al., 2015)

increased cell death | adult stage | progressive | somatic clone

(Huang et al., 2015)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

eye | somatic clone

(Huang et al., 2015)

eye photoreceptor cell | somatic clone

(Huang et al., 2015)

oocyte | germline clone

(Mirouse et al., 2006, Januschke et al., 2002)

retina | somatic clone

(Huang et al., 2015)

rhabdomere of eye photoreceptor cell | somatic clone

(Huang et al., 2015)

Detailed Description

Statement

Reference

DCTN2-p50^k16109 homozygous mutant clones in the eye have normal morphology at 1 day old and then show retinal degeneration, and ERG recordings show reduced on/off-transients; excision of the DCTN2-p50^k16109 P-element rescues phenotypes.

(Huang et al., 2015)

In most homozygous Dmn^k16109 germ-line clones, meiosis starts normally in four cells and is then restricted to two pro-oocytes in region 2a of the germarium. However, meiosis is never observed in region 3 and the cysts systematically fail to form an oocyte

(Mirouse et al., 2006)

Specification if the oocyte fails to occur in Dmn^k16109 germ line clone egg chambers, resulting in an egg chamber containing 16 nurse cells.

(Januschke et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dmn[+]/DCTN2-p50^k16109 is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Dmn[+]/DCTN2-p50^k16109 is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Dmn[+]/DCTN2-p50^k16109 is a non-enhancer of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Dmn[+]/DCTN2-p50^k16109 is a non-enhancer of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

Suppressor of

Statement

Reference

Dmn[+]/DCTN2-p50^k16109 is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Dmn[+]/DCTN2-p50^k16109 is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

Dmn[+]/DCTN2-p50^k16109 is a non-suppressor of aster | embryonic cycle 5 phenotype of CycB^+t10

(Ji et al., 2002)

Dmn[+]/DCTN2-p50^k16109 is a non-suppressor of aster | embryonic cycle 6 phenotype of CycB^+t10

(Ji et al., 2002)

Dmn[+]/DCTN2-p50^k16109 is a non-suppressor of aster | embryonic cycle 7 phenotype of CycB^+t10

(Ji et al., 2002)

Dmn[+]/DCTN2-p50^k16109 is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB^+t10

(Ji et al., 2002)

Other

Statement

Reference

Dmn[+]/DCTN2-p50^k16109, Dys^RNAi.C.UAS has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Dmn Dys double heterozygous flies (Dmn^k16109/Df(3R)Exel6184) do not exhibit indirect flight muscle degeneration.

One copy of Dmn^k16109 does not enhance the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of Dmn^k16109 does not enhance the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

Dmn^k16109 Dg³²³ double heterozygous flies do not exhibit indirect flight muscle degeneration.

(Kucherenko et al., 2011)

One copy of Dmn^k16109 moderately suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

(Kucherenko et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

l(2)k06006^k06006

(BDGP Project Members, 1994-1999)

Gγ1^k08017

(BDGP Project Members, 1994-1999)

Fails to complement

DCTN2-p50^k16218

(BDGP Project Members, 1994-1999)

Rescued by

DCTN2-p50^k16109 is rescued by Scer\GAL4^GMR.long/DCTN2-p50^UAS.cDa

(Huang et al., 2015)

Comments

Expression of DCTN2-p50^Scer\UAS.cDa driven by Scer\GAL4^GMR.long rescues ERG phenotypes (reduced on/off transients in response to light) in DCTN2-p50^k16109 homozygous mutant clones in the eye.

(Huang et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

11159

y¹ w^67c23; P{lacW}DCTN2-p50^k16109/CyO

Kyoto

111353

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}DCTN2-p50^k16109 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Precise excision of P{lacW}Dmn^k16109 can restore viability.

(Januschke et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DCTN2-p50^k16109

Dmn^k16109

(Kucherenko et al., 2011, Kucherenko et al., 2008, Yang et al., 2008, Christensen and Cook, 2007.3.22)

dmn^K16109

(Huang et al., 2015, Mirouse et al., 2006, Ji et al., 2002)

dynamitin^K16109

(Huynh and St. Johnston, 2004)

l(2)k16109

(Gunawardena et al., 2003, Spradling et al., 1999)

l(2)k16109^k16109

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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