Open Close
General Information
Symbol
Dmel\DysdsRNA.C.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0244100
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a hairpin loop which targets the common C-terminus, knocking down all Dys transcripts.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Myofibril integrity is disrupted in the indirect flight muscles (IMFs) of 3-5 days old flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU. The gross morphology of the IMFs is severely disrupted. The myofibril defects are significantly suppressed when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).

Overall activity of flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU is reduced compared to controls, and this is significantly suppressed when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).

Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU show significantly impaired recovery after being challenged by acute severe hypoxia exposure. The median time to recover from acute severe hypoxia is further increased if the flies have previously been exposed to chronic hypoxia.

Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4how-24B show significantly impaired recovery after being challenged by acute severe hypoxia exposure.

Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU have a significantly impaired climbing index, which is further reduced compared to wild type if the flies have previously been exposed to chronic hypoxia.

Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4how-24B have a significantly impaired climbing index compared to wild type.

Expression of DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU results in strong indirect flight muscle degeneration. Wing vein phenotypes are also seen; the posterior crossvein is drastically affected and extra material is seen above wing vein L2.

Flies expressing DysdsRNA.C.UAS under the control of Scer\GAL4how-24B experience lower heart failure rates in response to electrical pacing compared to controls throughout the first 4 weeks of adult life.

Expressing DysdsRNA.C.UAS under the control of Scer\GAL4tin.cBa has no impact on lifespan.

Retinal photoreceptor cells are not elongated in adult eyes expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.

Expression of DysdsRNA.C.Scer\UAS in the eye disc (under the control of Scer\GAL4GMR.PU), or in all glial cells (under the control of Scer\GAL4repo.PU), both result in photoreceptor axon targeting defects. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly. Expression of DysdsRNA.C.Scer\UAS in the mesodermal tissue (under the control of Scer\GAL4how-24B) does not effect the axon termination process.

Mis-localised glial cells are observed in the third instar larval eye discs of animals expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub. Although glial cells migrate correctly into the lamina, they are less organised and lack a clear separation of epithelial and marginal glial layers.

Flies expressing DysdsRNA.C.Scer\UAS under the control of either Scer\GAL4tub or the muscle-specific driver Scer\GAL4how-24B, show comparable climbing ability at the beginning of adult life to control flies. However, the ability of these animals to climb declines significantly faster over time compared to controls.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by mbl[+]/mblE27

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(2)k04222bk04222b/l(2)k04222b[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by nAChRα6EY13897/nAChRalpha6[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by v(2)k14204k14204/v(2)k14204[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(3)j5B6j5B6/l(3)j5B6[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by fra4/fra[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by sli2/sli[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by robo12/robo1[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by robo2[+]/robo2lea-2

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(2)k10317k10317/l(2)k10317[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by stan[+]/stan192

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Dadj1E4/Dad[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by msk[+]/msk5

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(3)L4092[+]/l(3)L4092L4092

Other
Phenotype Manifest In
Enhanced by
Suppressed by
Statement
Reference

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by Sply05091/Sply[+]

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by spin[+]/spin11F5

DysdsRNA.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by wun[+]/wunEMS4

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Expression of wunGD1640 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.

One copy of wunk10201 suppresses the gross defects in indirect flight muscle morphology seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.

One copy of wunEMS4 suppresses the gross defects in indirect flight muscle morphology seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.

One copy of Sply05091 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. This suppression is further enhanced when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).

Expression of laceScer\UAS.T:Ivir\HA1 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The gross defects in indirect flight muscle morphology and reduction in overall activity levels are also suppressed.

Co-expression of DysdsRNA.C.Scer\UAS and Sk1GD9386 under the control of Scer\GAL4tub.PU is lethal.

Co-expression of DysdsRNA.C.Scer\UAS and Sk2KK106572 under the control of Scer\GAL4tub.PU is lethal.

One copy of spin11F5 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
DysdsRNA.C.Scer\UAS
DysdsRNA.C.UAS
Name Synonyms
Secondary FlyBase IDs
    References (5)