Myofibril integrity is disrupted in the indirect flight muscles (IMFs) of 3-5 days old flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU. The gross morphology of the IMFs is severely disrupted. The myofibril defects are significantly suppressed when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).
Overall activity of flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU is reduced compared to controls, and this is significantly suppressed when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).
Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU show significantly impaired recovery after being challenged by acute severe hypoxia exposure. The median time to recover from acute severe hypoxia is further increased if the flies have previously been exposed to chronic hypoxia.
Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4how-24B show significantly impaired recovery after being challenged by acute severe hypoxia exposure.
Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU have a significantly impaired climbing index, which is further reduced compared to wild type if the flies have previously been exposed to chronic hypoxia.
Flies expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4how-24B have a significantly impaired climbing index compared to wild type.
Expression of DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.PU results in strong indirect flight muscle degeneration. Wing vein phenotypes are also seen; the posterior crossvein is drastically affected and extra material is seen above wing vein L2.
Flies expressing DysdsRNA.C.UAS under the control of Scer\GAL4how-24B experience lower heart failure rates in response to electrical pacing compared to controls throughout the first 4 weeks of adult life.
Expressing DysdsRNA.C.UAS under the control of Scer\GAL4tin.cBa has no impact on lifespan.
Retinal photoreceptor cells are not elongated in adult eyes expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub.
Expression of DysdsRNA.C.Scer\UAS in the eye disc (under the control of Scer\GAL4GMR.PU), or in all glial cells (under the control of Scer\GAL4repo.PU), both result in photoreceptor axon targeting defects. Axons stop irregularly, making gaps in the normal termination zone of the lamina plexus, deviating from the path and bundling aberrantly. Expression of DysdsRNA.C.Scer\UAS in the mesodermal tissue (under the control of Scer\GAL4how-24B) does not effect the axon termination process.
Mis-localised glial cells are observed in the third instar larval eye discs of animals expressing DysdsRNA.C.Scer\UAS under the control of Scer\GAL4tub. Although glial cells migrate correctly into the lamina, they are less organised and lack a clear separation of epithelial and marginal glial layers.
Flies expressing DysdsRNA.C.Scer\UAS under the control of either Scer\GAL4tub or the muscle-specific driver Scer\GAL4how-24B, show comparable climbing ability at the beginning of adult life to control flies. However, the ability of these animals to climb declines significantly faster over time compared to controls.
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by Sema1ak13702/Sema-1a[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by msk[+]/mskEMS-Mod90
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, enhanceable by gcmKG01117/gcm[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has abnormal locomotor behavior phenotype, suppressible by wun[+]/wunk10201
DysRNAi.C.UAS, Scer\GAL4Tub.PU has abnormal locomotor behavior phenotype, suppressible by wunGD1640, Scer\GAL4Tub.PU
DysRNAi.C.UAS, Scer\GAL4Tub.PU has abnormal locomotor behavior phenotype, suppressible by wun[+]/wunEMS4
DysRNAi.C.UAS, Scer\GAL4Tub.PU has abnormal locomotor behavior phenotype, suppressible by laceUAS.Tag:HA, Scer\GAL4Tub.PU
DysRNAi.C.UAS, Scer\GAL4Tub.PU has abnormal locomotor behavior phenotype, suppressible by spin[+]/spin11F5
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by mbl[+]/mblE27
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(2)k04222bk04222b/l(2)k04222b[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by nAChRα6EY13897/nAChRalpha6[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by v(2)k14204k14204/v(2)k14204[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Lis-1[+]/Lis-1k13209
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Khck13219/Khc[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Dmn[+]/DCTN2-p50k16109
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(3)j5B6j5B6/l(3)j5B6[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Sema-2a[+]/Sema2aunspecified
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by fra4/fra[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by sli2/sli[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by robo12/robo1[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by robo2[+]/robo2lea-2
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Sdc10608/Sdc[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(2)k10317k10317/l(2)k10317[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by stan[+]/stan192
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by wg[+]/wgspd-1
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by grhs2140/grh[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by DeltaEMS-Mod130/Dl[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by DeltaEMS-Mod140/Dl[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Dadj1E4/Dad[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by dppKG08191/dpp[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by msk[+]/msk5
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by kek1[+]/kek1k07332
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by Nrk[+]/Nrkk14301
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by HipkBG00855/hipk[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by kisk13416/kis[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by CG4496[+]/CG4496KG10365
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by wun[+]/wunk10201
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by POSH[+]/POSHk15815
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by vimark16722/vimar[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by delKG10262/del[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by uifEMS-Mod29/uif[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by CG7845[+]/l(2)k09848EMS-Mod4
DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype, suppressible by l(3)L4092[+]/l(3)L4092L4092
DysRNAi.C.UAS, Scer\GAL4Tub.PU, Sk1GD9386 has lethal phenotype
DysRNAi.C.UAS, Scer\GAL4Tub.PU, Sk2KK106572 has lethal phenotype
DgRNAi.UAS, DysRNAi.C.UAS, Scer\GAL4Tub.PU has visible phenotype
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, enhanceable by Sema1ak13702/Sema-1a[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, enhanceable by msk[+]/mskEMS-Mod90
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, enhanceable by gcmKG01117/gcm[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has indirect flight muscle cell phenotype, suppressible by wunGD1640, Scer\GAL4Tub.PU
DysRNAi.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by wunGD1640, Scer\GAL4Tub.PU
DysRNAi.C.UAS, Scer\GAL4Tub.PU has indirect flight muscle cell phenotype, suppressible by spin[+]/spin11F5
DysRNAi.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by spin[+]/spin11F5
DysRNAi.C.UAS, Scer\GAL4Tub.PU has indirect flight muscle cell phenotype, suppressible by wun[+]/wunk10201
DysRNAi.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by wun[+]/wunk10201
DysRNAi.C.UAS, Scer\GAL4Tub.PU has indirect flight muscle cell phenotype, suppressible by wun[+]/wunEMS4
DysRNAi.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by wun[+]/wunEMS4
DysRNAi.C.UAS, Scer\GAL4Tub.PU has indirect flight muscle cell phenotype, suppressible by Sply05091/Sply[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by Sply05091/Sply[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has indirect flight muscle cell phenotype, suppressible by laceUAS.Tag:HA, Scer\GAL4Tub.PU
DysRNAi.C.UAS, Scer\GAL4Tub.PU has myofibril phenotype, suppressible by laceUAS.Tag:HA, Scer\GAL4Tub.PU
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by mbl[+]/mblE27
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by l(2)k04222bk04222b/l(2)k04222b[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by nAChRα6EY13897/nAChRalpha6[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by v(2)k14204k14204/v(2)k14204[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Lis-1[+]/Lis-1k13209
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Khck13219/Khc[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Dmn[+]/DCTN2-p50k16109
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by l(3)j5B6j5B6/l(3)j5B6[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Sema-2a[+]/Sema2aunspecified
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by fra4/fra[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by sli2/sli[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by robo12/robo1[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by robo2[+]/robo2lea-2
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Sdc10608/Sdc[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by l(2)k10317k10317/l(2)k10317[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by stan[+]/stan192
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by wg[+]/wgspd-1
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by grhs2140/grh[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by DeltaEMS-Mod130/Dl[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by DeltaEMS-Mod140/Dl[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Dadj1E4/Dad[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by dppKG08191/dpp[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by msk[+]/msk5
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by kek1[+]/kek1k07332
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by Nrk[+]/Nrkk14301
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by HipkBG00855/hipk[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by kisk13416/kis[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by CG4496[+]/CG4496KG10365
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by wun[+]/wunk10201
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by POSH[+]/POSHk15815
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by vimark16722/vimar[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by delKG10262/del[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by uifEMS-Mod29/uif[+]
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by CG7845[+]/l(2)k09848EMS-Mod4
DysRNAi.C.UAS, Scer\GAL4Tub.PU has posterior crossvein phenotype, suppressible by l(3)L4092[+]/l(3)L4092L4092
DgRNAi.UAS, DysRNAi.C.UAS, Scer\GAL4Tub.PU has wing vein L3 phenotype
Dmn[+]/DCTN2-p50k16109, DysRNAi.C.UAS has wing vein | ectopic phenotype
DysRNAi.C.UAS, l(3)j5B6j5B6/l(3)j5B6[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, Sema-2a[+]/Sema2aunspecified has wing vein | ectopic phenotype
DysRNAi.C.UAS, fra4/fra[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, sli2/sli[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, robo12/robo1[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, robo2[+]/robo2lea-2 has wing vein | ectopic phenotype
DysRNAi.C.UAS, Sdc10608/Sdc[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, l(2)k10317k10317/l(2)k10317[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, stan[+]/stan192 has wing vein | ectopic phenotype
DysRNAi.C.UAS, wg[+]/wgspd-1 has wing vein | ectopic phenotype
DysRNAi.C.UAS, grhs2140/grh[+] has wing vein | ectopic phenotype
DeltaEMS-Mod130/Dl[+], DysRNAi.C.UAS has wing vein | ectopic phenotype
DeltaEMS-Mod140/Dl[+], DysRNAi.C.UAS has wing vein | ectopic phenotype
Dadj1E4/Dad[+], DysRNAi.C.UAS has wing vein | ectopic phenotype
DysRNAi.C.UAS, dppKG08191/dpp[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, msk[+]/msk5 has wing vein | ectopic phenotype
DysRNAi.C.UAS, kek1[+]/kek1k07332 has wing vein | ectopic phenotype
DysRNAi.C.UAS, Nrk[+]/Nrkk14301 has wing vein | ectopic phenotype
DysRNAi.C.UAS, HipkBG00855/hipk[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, kisk13416/kis[+] has wing vein | ectopic phenotype
CG4496[+]/CG4496KG10365, DysRNAi.C.UAS has wing vein | ectopic phenotype
DysRNAi.C.UAS, wun[+]/wunk10201 has wing vein | ectopic phenotype
DysRNAi.C.UAS, POSH[+]/POSHk15815 has wing vein | ectopic phenotype
DysRNAi.C.UAS, vimark16722/vimar[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, delKG10262/del[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, uifEMS-Mod29/uif[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, CG7845[+]/l(2)k09848EMS-Mod4 has wing vein | ectopic phenotype
DysRNAi.C.UAS, l(3)L4092[+]/l(3)L4092L4092 has wing vein | ectopic phenotype
DysRNAi.C.UAS, Sema1ak13702/Sema-1a[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, mbl[+]/mblE27 has wing vein | ectopic phenotype
DysRNAi.C.UAS, msk[+]/mskEMS-Mod90 has wing vein | ectopic phenotype
DysRNAi.C.UAS, gcmKG01117/gcm[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, l(2)k04222bk04222b/l(2)k04222b[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, nAChRα6EY13897/nAChRalpha6[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, v(2)k14204k14204/v(2)k14204[+] has wing vein | ectopic phenotype
DysRNAi.C.UAS, Lis-1[+]/Lis-1k13209 has wing vein | ectopic phenotype
DysRNAi.C.UAS, Khck13219/Khc[+] has wing vein | ectopic phenotype
Expression of wunGD1640 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.
One copy of wunk10201 suppresses the gross defects in indirect flight muscle morphology seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.
One copy of wunEMS4 suppresses the gross defects in indirect flight muscle morphology seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.
One copy of Sply05091 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. This suppression is further enhanced when the flies are given food supplemented with 2-acetyl-4(5)-tetrahydroxybutyl imidazole (THI).
Expression of laceScer\UAS.T:Ivir\HA1 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The gross defects in indirect flight muscle morphology and reduction in overall activity levels are also suppressed.
Co-expression of DysdsRNA.C.Scer\UAS and Sk1GD9386 under the control of Scer\GAL4tub.PU is lethal.
Co-expression of DysdsRNA.C.Scer\UAS and Sk2KK106572 under the control of Scer\GAL4tub.PU is lethal.
One copy of spin11F5 suppresses the defects in indirect flight muscle myofibril integrity seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU. The reduction in activity levels is also suppressed.