mNeonGreen is a monomeric yellow-green fluorescent protein with an excitation peak of 506nm and an emission peak of 517nm in vitro and is characterized by high brightness. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Branchiostoma lanceolatum blFP-Y3 gene, containing the following mutations relative to blFP-Y3: F15I, R25Q, A45D, Q56H, F67Y, K79V, S100V, F115A, I118K, V140R, T141S, M143K, L144T, D156K, T158S, S163N, Q168R, V171A, N174T, I185Y and F192Y, in addition to appended EGFP-type termini (MVSKGEEDNMA at the N-terminal end, GMDELYK at the C-terminal end) (PMID:23524392).
