FlyBase Experimental Tool Report: mKO2

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Experimental tool: mKO2

General Information

Symbol

mKO2

FlyBase ID

FBto0000132

Name

monomeric Kusabira-Orange variant 2

Description

Description

mKO2 is a monomeric orange fluorescent protein with an excitation peak of 551nm and an emission peak of 565nm in vitro (PMID:20664080). It is a fast-folding derivative of mKO (PMID:18267078).

Uses

orange fluorescent protein

External Crossreferences and Linkouts

FPbase - A database for users of fluorescent proteins.

134NN

Related experimental tools

Common gene of origin (3)

Tool

Uses

Kusabira-Orange

orange fluorescent protein

orange fluorescent protein

orange fluorescent protein

Transgenic Constructs

Encodes tool (3)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{HmlΔ-mKO2-CDT1}

Vcon\KO^{mKO2.Hml.Δ.Tag:degron(hCDT1-30-120)}

Hml

mKO2: orange fluorescent protein

Tag:degron(hCDT1-30-120): cell cycle-regulated degradation tag

P{UAS-mKO2.P}

Vcon\KO^mKO2.UAS.cPa

UASt

mKO2: orange fluorescent protein

P{UASp-mKO2-CDT1-UD}

Vcon\KO^{mKO2.hCdt1-UD.UASp}

UASp

mKO2: orange fluorescent protein

Tagged with the tool (9)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{UAS-CtsL1.mKO2}

UASt

CtsL1

mKO2: orange fluorescent protein

P{UAS-mh.mKO2}

UASt

mh

mKO2: orange fluorescent protein

P{UASp-mh.mKO2}

UASp

mh

mKO2: orange fluorescent protein

P{UAS-Prosα6.mKO2}

Prosα6^UAS.mKO2

UASt

Prosα6

mKO2: orange fluorescent protein

P{UAS-tau.mKO2}

UASt

tau

mKO2: orange fluorescent protein

P{UAS-Vha13.mKO2}

UASt

Vha13

mKO2: orange fluorescent protein

P{UAS-Vps26.mKO2}

UASt

Vps26

mKO2: orange fluorescent protein

P{UAS-wupA.mKO2}

UASt

wupA

mKO2: orange fluorescent protein

PBac{10xUAS-mito-mKO2-V5-APEX2}

Gmax\APX1^{APEX2.10xUAS.Tag:Mito(Unk).Tag:V5.mKO2}

UAS

APEX2: reporter enzyme
proximity labeling tool (protein)

Tag:V5: epitope tag
mKO2: orange fluorescent protein
Tag:Mito(Unk): mitochondrial protein localization tag

Carrying the tool (1)

Transgenic construct(s)

Component allele

Reg. region

Encoded product / tool

Tagged with

Also carries

Stocks

P{UAS-Cam.mKO2}

UASt

Cam

mKO2: orange fluorescent protein

Insertions ( 18 )

Insertion

Encoded product / tool

Tagged with

Also carries

Stocks

Associated Alleles

P{UAS-Brainbow}attP2

EBFP2: blue fluorescent protein
EGFP: green fluorescent protein
mKO2: orange fluorescent protein

Tag:HA: epitope tag
Tag:MYC: epitope tag
Tag:V5: epitope tag

loxP: recombinase target site
lox2272: recombinase target site
lox5171: recombinase target site

P{UAS-Brainbow}attP40

EBFP2: blue fluorescent protein
EGFP: green fluorescent protein
mKO2: orange fluorescent protein

Tag:HA: epitope tag
Tag:MYC: epitope tag
Tag:V5: epitope tag

loxP: recombinase target site
lox2272: recombinase target site
lox5171: recombinase target site

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mKO2

mKusabira-Orange2

(FlyBase, 2018-)

Name Synonyms

monomeric Kusabira-Orange variant 2

Secondary FlyBase IDs

References (11)

Report Sections