Pros35, α6, PROS-35, Proteasome 35kD subunit, anon- SAGE:Wang-116
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.52
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Interacts with PI31.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prosα6 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Prosα6 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Prosα6 Pros35
Source for merge of: Pros35 anon- SAGE:Wang-116
The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
The Pros35 gene has been cloned and sequenced, and its genomic organisation has been analysed. Analysis of Pros35-Ppyr\LUC promoter deletion constructs shows that the Pros35 promoter region between -605 and -330bp contains sequences that are important for Pros35 gene activity, and that deletions beyond -150bp result in an almost complete inhibition of transcription.
The cellular distribution of the proteasome during embryogenesis has been investigated using an antibody that recognises Pros35 proteasome subunit.
Encodes the 35kD proteasome subunit of D.melanogaster. The proteasome is strongly expressed in the central nervous system of embryos and the heart muscle and epithelial cells of the stomach and ovary of adults and is localized in the cytoplasm in some cells and/or the nucleus in others.