| General Information | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Term | recombinase | ID (Ontology) | FBcv:0005062 (FlyBase CV) | |||||||||||||||||||
| Definition | An enzyme that mediates a recombination exchange reaction between two DNA templates, each containing a specific recognition site. The recognition site for a particular site-specific recombinase is defined by the enzyme itself. The recognition site contains perfect inverted repeats flanking an asymmetric spacer. Under normal conditions, the two recognition sites are identical and are reformed during the recombination event, and thus the reaction is bidirectional: recombination can occur in both 'forward' and 'reverse' directions. However, recognition sites can be mutated to drive the directionality of the reaction: different classes of site may be combined so that recombination can occur in one direction, but produces reformed sites that are no longer compatible. In addition, mutually exclusive sites have been engineered for some recombinases. Recombinases can be used to generate many different types of genetic modification, with the outcome being influenced by the relative orientation (direct or inverted), relative location and composition of the two recognition sites. The types of possible modification include deletion of DNA, generation of chromosomal rearrangements, integration of DNA into the genome, and replacement of genetic material with that from a donor plasmid using recombination-mediated cassette exchange (RMCE).[ FlyBase:FBrf0231034 ] | |||||||||||||||||||||
| Also Known As | "site-specific recombinase" | |||||||||||||||||||||
| Comment | ||||||||||||||||||||||
| Links to External Ontologies | ||||||||||||||||||||||
| Annotations | ||||||||||||||||||||||
| Records annotated with this term OR any of its CHILD TERMS | ||||||||||||||||||||||
|
Choose a record type to view annotations for this term and its child terms. Direct annotations and full annotation statements below also provide HitList buttons. Results list data from multiple species. Choose a record type above to open its HitList. |
||||||||||||||||||||||
| Direct annotations: records annotated with this exact term (child terms are NOT included) | ||||||||||||||||||||||
| There are no annotations using this term. | ||||||||||||||||||||||
Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
|
||||||||||||||||||||||
|
||||||||||||||||||||||
|
||||||
experimental tool descriptor |__genome engineering tool |__recombinase 250 rec. |__conditional recombinase 5 rec. |__small molecule-regulated recombinase 5 rec. |
| Spanning Tree View Settings | |||
|---|---|---|---|
| Parents/Children View Depth |
|||
Relationships
|
|||
| Is a | genome engineering tool | ||
| Part of | |||
|
Synonyms & Secondary IDs
|
|||
| Synonyms | |||
|
|||
| Secondary IDs | |||
|
|
|||
|
External Crossreferences & Linkouts
|
|||
