α-tubulin, tubulin, α-Tub67C, α4-tubulin, α-Tub
Gene model reviewed during 5.43
Gene model reviewed during 5.46
Gene model reviewed during 5.55
There is only one protein coding transcript and one polypeptide associated with this gene
462 (aa); 55 (kD predicted)
The location of maternal and constitutive
α-tubulins is indistinguishable until ~8 hrs of development as determined
by staining with maternal α-tubulin-specific and general α-tubulin
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\αTub67C using the Feature Mapper tool.
αTub67C protein is most abundant in early embryos. Levels decline between 6 and 9 hours and it is nearly undetectable after 18hr. By 8hrs, maternal tubulin has diminished in most cells of the embryo but is retained in the ventral midline. At 15hrs, staining occurs only in a single pair of connective fibers on either side of the midline.
To compare synthesis and accumulation of α-tubulin proteins, 35S</up>methionine radiolabeled tissues were subjected to Western analysis using monoclonal anti-α-tubulin antibody. A stable pool of αTub67C protein was detected in ovaries, unfertilized eggs, and embryos, but synthesis was detected only in ovaries.
GBrowse - Visual display of RNA-Seq signalsView Dmel\αTub67C in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: αTub67C CG8308
RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cell results in dim staining of microtubules, and a short monopolar spindle. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
In a sample of 79 genes with multiple introns, 33 showed significant heterogeneity in G+C content among introns of the same gene and significant positive correspondence between the intron and the third codon position G+C content within genes. These results are consistent with selection adding against preferred codons at the start of genes.
The αTub67C gene product is involved in the formation of the sperm aster, cleavage spindle apparatus formation or function and the differentiation of the embryonic nervous system.
Mutant females produce androgenetic offspring, the mutant protein results in an aberrant oocyte- and early-embryo-specific α-tubulin. The production of androgenetic exceptions is greatly enhanced when the parental females are heterozygous for αTub67C and homozygous for ncdD.
A PCR based assay has been used to determine whether the encoded mRNAs exhibit changes in poly(A) status upon translational activation.
αTub67C is required for nuclear division in the oocyte and early embryo. Both meiosis and cleavage stage mitoses are severely affected by mutations that result in a substantial decrease in the αTub67C/αTub84B+αTub84D ratio, though an increase has little effect on meiosis while still disrupting mitotic spindle formation.
This maternally provided tubulin is found in all classes of microtubule from syncitial blastoderm to completion of germ band retraction, subsequently it is only retained in the CNS. Its localisation is not affected by mutations in other tubulins.
In D.melanogaster, two multigene families, each made up of four members, code for α- and β-tubulins. Tubulins are a highly conserved family of proteins that are the main structural components of microtubules in mitotic and meiotic spindles, cilia, flagella, neural processes and the cytoskeleton; nontubulin proteins (MAPS or microtubule-associated proteins) are involved along with tubulins in the formation of specialized microtubules (FBrf0045282; FBrf0046966). αTub67C is transcribed in the nurse cells, the transcript is maternally inherited; the transcript accumulates in 0- to 3-hr embryos and adult ovaries (FBrf0037623; FBrf0049502). A stable pool of αTub67C tubulin is found in ovaries, unfertilized eggs and embryos, but synthesis of the protein probably only occurs in the ovary (FBrf0049502).
Loss-of-function mutations are female sterile. These alleles show a dominant reduction in female fertility (FBrf0058107).
Mutation is named "Tomaj" after a Hungarian clan that vanished by the beginning of the 14th century but their names survived in the names of settlements.
Named for a Hungarian family that vanished by the beginning of the 14th century.