FlyBase Allele Report: Dmel\αTub67C[P40]

General Information

Symbol

Dmel\αTub67C^P40

Species

D. melanogaster

Name

FlyBase ID

FBal0098573

Feature type

allele

Associated gene

Dmel\αTub67C

Associated Insertion(s)

P{lacW}αTub67C^P40

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Sekelsky et al., 1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}αTub67C^P40

Cytology

Description

P{lacW} is inserted into the αTub67C transcription unit, 36bp from the 3' end.

(Sekelsky et al., 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | dominant

(Matthies et al., 1999, Sekelsky et al., 1999)

female sterile | recessive

(Sekelsky et al., 1999)

Phenotype Manifest In

meiotic metaphase I

(Sekelsky et al., 1999)

oocyte

(Matthies et al., 1999, Sekelsky et al., 1999)

oocyte & chromatin

(Matthies et al., 1999)

Detailed Description

Statement

Reference

The spindles of heterozygous oocytes are on average somewhat shorter than wild-type spindles. There are no obvious defects in spindle structure, however, there is a failure of chromatin mass to elongate along the axis of the spindle. The chromatin remains almost spherical, even on fully elongated spindles, in contrast to wild type where the chromatin mass elongates as spindle assembly progresses. Heterozygous females show 2-fold higher levels of X chromosome nondisjunction than normal, which are mostly if not all due to a failure of achiasmate segregation. Heterozygous females also show elevated levels of 4th chromosome nondisjunction (most of these 4th chromosome nondisjunction events are a secondary consequence of failures of X chromosome missegregation).

(Matthies et al., 1999)

Mutant for female meiotic segregation: 1.6% X chromosome exceptions from X/X females. 0.8% 4th chromosome exceptions from X/X females. 27.6% X chromosome exceptions from X/Bal females. 19.4% 4th chromosome exceptions from X/Bal females. Axs-like mutant. Primarily affects achiasmate chromosome segregation. Only the segregation of achiasmate chromosomes is affected. Rate of X chromosome nondisjunction is two to three fold higher than that of 4th chromosome nondisjunction. X and 4th chromosome nondisjunction show a strong positive correlation. The majority of simultaneous X and 4th chromosome nondisjunction is non-homologous XX - 44 segregation. Exchange is normal. Cytological studies reveal a defect in the progression of the oocytes from metaphase I to anaphase I.

(Sekelsky et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

αTub67C^P40 has abnormal meiotic cell cycle | dominant phenotype, suppressible by nod³

(Matthies et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

αTub67C^P40 has phenotype, suppressible by Df(1)nod

(Matthies et al., 1999)

αTub67C^P40 has oocyte & chromatin phenotype, suppressible by nod³

(Matthies et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The defect in chromatin elongation but not the spindle length defect seen in heterozygotes is suppressed if the females are also heterozygous for nod³. The meiotic defects of heterozygotes are strongly suppressed if they are also heterozygous for nod³.

(Matthies et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

αTub67C^unspecified

(Sekelsky et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Mobilisation of the P{lacW} can revert both the female sterility and the meiotic phenotype, simultaneously.

(Sekelsky et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

mei-P40

(Sekelsky et al., 1999)

αTub67C^P40

αtub67C^P40

(Matthies et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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