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FB2026_01
,
released March 12, 2026
spindle | ectopic (with αTub67Cunspecified)
Heterozygous females have reduced fertility; when mated to wild-type males, approximately 37.2% of the progeny show early embryonic arrest (they fail to make cuticle), approximately 18.0% show late embryonic arrest (embryos make cuticle or show other obvious evidence of post-gastrulation development, but fail to hatch) and approximately 44.8% of the embryos are apparently normal and hatch.
Eggs derived from females transheterozygous for αTub67Cunspecified and αTub67C3 have abnormal gonomeric spindles. Abnormal ectopic cleavage spindles form, the abnormality becoming more severe with time.
Embryos derived from homozygous females have a single large polar body with greater than 4 haploid sets of chromosomes. Oocytes of heterozygous females complete the meiotic divisions but are defective in female pronucleus formation.
No eggs from hemizygous females hatch. Many are apparently unfertilized, though insemination occurs. Paternal genotype has no effect on viability of progeny. Majority of eggs had 3 or fewer nuclei. This allele also shows a dominant reduction in female fertility.
αTub67C3 has female sterile | semidominant phenotype, suppressible by swa[+]/swaVA11
αTub67C3 has female sterile | semidominant phenotype, suppressible by swa[+]/swa1
αTub67C3 has female sterile | semidominant phenotype, suppressible by swa3/swa[+]
αTub67C3 has female sterile | semidominant phenotype, suppressible by swa[+]/swa4
αTub67C3 has female sterile | semidominant phenotype, suppressible by swa[+]/swa6
αTub67C3 has female sterile | semidominant phenotype, suppressible by swa[+]/swaTG31
αTub67C3 has female sterile | semidominant phenotype, non-suppressible by hts10089/hts[+]
αTub67C3 has female sterile | semidominant phenotype, non-suppressible by hts1e25/hts[+]
αTub67C3 has female sterile | semidominant phenotype, non-suppressible by swaTN62/swa[+]
αTub67C3 has female sterile | semidominant phenotype, non-suppressible by Df(1)JF5/+
αTub67C3, ncd2 has female sterile phenotype
αTub67C3, ncd1 has female sterile phenotype
αTub67C3, ncdD has abnormal meiotic cell cycle | oogenesis phenotype
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by swa3/swa[+]
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by swa[+]/swa4
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by swa[+]/swa6
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by swa[+]/swaTG31
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by swa[+]/swaVA11
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by swa[+]/swa1
αTub67C3 has phenotype, suppressible by nodunspecified
αTub67C3 has embryonic/first instar larval cuticle | maternal effect phenotype, non-suppressible by swaTN62/swa[+]
Approximately 58.5% of embryos derived from swa1/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ female flies. Approximately
29.4% of embryos derived from swa1/+;67C3/+ females arrest development
before cuticle formation, compared to approximately 37.2% of embryos
derived from αTub67C3/+ female flies. Approximately 12.1% of
embryos derived from swa1/+;αTub67C3/+ female flies arrest
development after cuticle formation but before hatching, compared to
approximately 18% in αTub67C3/+ female derived embryos.
>
Approximately 58.5% of embryos derived from swa1/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
29.4% of embryos derived from swa1/+;67C3/+ female flies arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ female flies. Approximately
12.1% of embryos derived from swa1/+;αTub67C3/+ females
arrest development after cuticle formation but before hatching, compared
to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 65.9% of embryos derived from swa3/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
27.1% of embryos derived from swa3/+;67C3/+ females arrest development
before cuticle formation, compared to approximately 37.2% of embryos
derived from αTub67C3/+ females. Approximately 7.0% of embryos
derived from swa3/+;αTub67C3/+ females arrest development
after cuticle formation but before hatching, compared to approximately
18% in αTub67C3/+ female derived embryos.
>
Approximately 65.9% of embryos derived from swa3/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
27.1% of embryos derived from swa3/+;67C3/+ females arrest development
before cuticle formation, compared to approximately 37.2% of embryos
derived from αTub67C3/+ females. Approximately 7.0% of embryos
derived from swa3/+;αTub67C3/+ females arrest development
after cuticle formation but before hatching, compared to approximately
18% in embryos derived from αTub67C3/+ females.
Approximately 60.3% of embryos derived from swa6/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
35.1% of embryos derived from swa6/+;67C3/+ females arrest development
before cuticle formation, compared to approximately 37.2% of embryos
derived from αTub67C3/+ females. Approximately 4.6% of embryos
derived from swa6/+;αTub67C3/+ females arrest development
after cuticle formation but before hatching, compared to approximately
18% in αTub67C3/+ female derived embryos.
>
Approximately 60.3% of embryos derived from swa6/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
35.1% of embryos derived from swa6/+;67C3/+ females arrest development
before cuticle formation, compared to approximately 37.2% of embryos
derived from αTub67C3/+ females. Approximately 4.6% of embryos
derived from swa6/+;αTub67C3/+ females arrest development
after cuticle formation but before hatching, compared to approximately
18% in embryos derived from αTub67C3/+ females.
Approximately 87% of embryos derived from swaTG31/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
7.7% of embryos derived from swaTG31/+;67C3/+ females arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ females. Approximately 5.2%
of embryos derived from swaTG31/+;αTub67C3/+ females arrest
development after cuticle formation but before hatching, compared to
approximately 18% in αTub67C3/+ female derived embryos.
>
Approximately 87% of embryos derived from swaTG31/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
7.7% of embryos derived from swaTG31/+;67C3/+ females arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ females. Approximately 5.2%
of embryos derived from swaTG31/+;αTub67C3/+ females arrest
development after cuticle formation but before hatching, compared to
approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 36.1% of embryos derived from swaTN62/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
48.0% of embryos derived from swaTN62/+;67C3/+ females arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ females. Approximately 15.8%
of embryos derived from female swaTN62/+;αTub67C3/+ flies
arrest development after cuticle formation but before hatching, compared
to approximately 18% in αTub67C3/+ female derived embryos.
>
Approximately 36.1% of embryos derived from swaTN62/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
48.0% of embryos derived from swaTN62/+;67C3/+ females arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ females. Approximately 15.8%
of embryos derived from female swaTN62/+;αTub67C3/+ flies
arrest development after cuticle formation but before hatching, compared
to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 66.2% of embryos derived from swaVA11/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
23.4% of embryos derived from swaVA11/+;67C3/+ female flies arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ females. Approximately 10.4%
of embryos derived from swaVA11/+;αTub67C3/+ female flies
arrest development after cuticle formation but before hatching, compared
to approximately 18% in αTub67C3/+ female derived embryos.
>
Approximately 66.2% of embryos derived from swaVA11/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
23.4% of embryos derived from swaVA11/+;67C3/+ female flies arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ females. Approximately 10.4%
of embryos derived from swaVA11/+;αTub67C3/+ female flies
arrest development after cuticle formation but before hatching, compared
to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 68.5% of embryos derived from swa4/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
23.2% of embryos derived from swa4/+;67C3/+ female flies arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ female flies. Approximately
8.3% of embryos derived from swa4/+;αTub67C3/+ female flies
arrest development after cuticle formation but before hatching, compared
to approximately 18% in αTub67C3/+ female derived embryos.
>
Approximately 68.5% of embryos derived from swa4/+;αTub67C3/+
females hatch to apparently normal embryos, compared to approximately
44.8% of embryos derived from αTub67C3/+ females. Approximately
23.2% of embryos derived from swa4/+;67C3/+ female flies arrest
development before cuticle formation, compared to approximately 37.2%
of embryos derived from αTub67C3/+ female flies. Approximately
8.3% of embryos derived from swa4/+;αTub67C3/+ female flies
arrest development after cuticle formation but before hatching, compared
to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 58.5% of embryos derived from swa1/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 29.4% of embryos derived from swa1/+;67C3/+ female flies arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ female flies. Approximately 12.1% of embryos derived from swa1/+;αTub67C3/+ females arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 65.9% of embryos derived from swa3/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 27.1% of embryos derived from swa3/+;67C3/+ females arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ females. Approximately 7.0% of embryos derived from swa3/+;αTub67C3/+ females arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 60.3% of embryos derived from swa6/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 35.1% of embryos derived from swa6/+;67C3/+ females arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ females. Approximately 4.6% of embryos derived from swa6/+;αTub67C3/+ females arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 87% of embryos derived from swaTG31/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 7.7% of embryos derived from swaTG31/+;67C3/+ females arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ females. Approximately 5.2% of embryos derived from swaTG31/+;αTub67C3/+ females arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 36.1% of embryos derived from swaTN62/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 48.0% of embryos derived from swaTN62/+;67C3/+ females arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ females. Approximately 15.8% of embryos derived from female swaTN62/+;αTub67C3/+ flies arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 66.2% of embryos derived from swaVA11/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 23.4% of embryos derived from swaVA11/+;67C3/+ female flies arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ females. Approximately 10.4% of embryos derived from swaVA11/+;αTub67C3/+ female flies arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
Approximately 68.5% of embryos derived from swa4/+;αTub67C3/+ females hatch to apparently normal embryos, compared to approximately 44.8% of embryos derived from αTub67C3/+ females. Approximately 23.2% of embryos derived from swa4/+;67C3/+ female flies arrest development before cuticle formation, compared to approximately 37.2% of embryos derived from αTub67C3/+ female flies. Approximately 8.3% of embryos derived from swa4/+;αTub67C3/+ female flies arrest development after cuticle formation but before hatching, compared to approximately 18% in embryos derived from αTub67C3/+ females.
αTub67C3/+ ncdD/ncdD embryos have both a male and female pronucleus, but have defects in pronuclear conjugation or fusion and show delayed meiotic progression. ncdD/ncdD enhances the production of exceptional androgenetic females by αTub67C3 heterozygotes. αTub67C3 dominantly enhances the frequencies of nondisjunction and zygotic loss of the X chromosome in ncdD/ncdD flies.
Embryos derived from D.melanogaster αTub67C3 ncdD/ncdD females mated to D.simulans males show a hatch rate of 24.9%. Of the hatched embryos, 24.9% develop to adulthood. Of the adult hybrid progeny, 0.4% are androgenetic females and 6.1% are X0 males or gynandromorphs.
αTub67C3 is rescued by αTub67Cnanos.PT
αTub67C3/αTub67CRX2.1 is rescued by αTub67C+t7.3
αTub67C3/αTub67C1 is rescued by αTub67C+t7.3
αTub67C3 is rescued by αTub67C+t7
Rees.
Based on average degree of development of eggs of the hemizygous mutant genotype the alleles can be ranked, from most to least severe, in the order αTub67C2 < αTub67C4 < αTub67C1 < αTub67C3. Based on the semidominant phenotypes the rank order is αTub67C3 < αTub67C1 = αTub67C2 = αTub67C4.