Isolation and characterization of Df(3L)BSC392
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC392 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}fryf01845and P{XP}nudEd10518. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}fryf01845/P{XP}nudEd10518 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC392 from the segment of PBac{WH}fryf01845 to the left of its FRT site and the segment of P{XP}nudEd10518 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC392 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 67C4;67D1. Df(3L)BSC392 failed to complement alphaTub67C1. Df(3L)FDD-0279885 is a synonym for Df(3L)BSC392.