FlyBase Allele Report: Dmel\αTub67C[GD13885]

General Information

Symbol

Dmel\αTub67C^GD13885

Species

D. melanogaster

Name

FlyBase ID

FBal0198549

Feature type

allele

Associated gene

Dmel\αTub67C

Associated Insertion(s)

Carried in Construct

P{GD13885}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13885}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13885

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of αTub67C^GD13885 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of Scer\GAL4^insc-Mz1407 results in formation of ectopic neuroblast in both type I lineages and type II lineages in third instar larval brains but does not disrupt the normal asymmetric protein (aPKC, mira) localization in neuroblasts.

(Zhang et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ may result in an extreme increase in neuroblast size in the larval brain, without loss of neuroblasts (depending on the insertion line used).

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in defects in the shape of neuroblasts in the larval brain (the severity depends on the insertion line used).

(Neumüller et al., 2011)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13885

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

αTub67C^GD13885, Scer\GAL4^insc-Mz1407 has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

αTub67C^GD13885, Scer\GAL4^insc-Mz1407 has increased cell number | third instar larval stage phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/αTub67C^GD13885 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Scer\GAL4^insc-Mz1407/αTub67C^GD13885 is a suppressor of decreased cell number | third instar larval stage phenotype of pins^P89

(Zhang et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

αTub67C^GD13885, Scer\GAL4^insc-Mz1407 has type I neuroblast | third instar larval stage | increased number phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

αTub67C^GD13885, Scer\GAL4^insc-Mz1407 has type II neuroblast | third instar larval stage | increased number phenotype, enhanceable by pins^P89

(Zhang et al., 2016)

Enhancer of

Statement

Reference

αTub67C^GD13885, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Enhancer of

Statement

Reference

αTub67C^GD13885, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

NOT Suppressor of

Statement

Reference

αTub67C^GD13885, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.PF

(Butzlaff et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of brain neuroblasts observed in third instar larvae expressing αTub67C^GD13885 under the control of Scer\GAL4^insc-Mz1407 is increased further by combination with pins^P89 (although the pins^P89 mutants on their own display significantly lower number of brain neuroblasts compared to controls) and leads to a dramatic neuroblast overgrowth as well as protein segregation defects in the neuroblasts (aPKC, mira) during metaphase and their mis-segregation in telophase.

(Zhang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v24783