FlyBase Gene Report: Dmel\Spps

General Information

Symbol

Dmel\Spps

Species

D. melanogaster

Name

Sp1-like factor for pairing sensitive-silencing

Annotation Symbol

CG5669

Feature Type

protein_coding_gene

FlyBase ID

FBgn0039169

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Sp1-like factor for pairing sensitive-silencing (Spps) encodes a zinc-finger protein in the Sp1/KLF family. It binds to Polycomb response elements, and is required for the recruitment of Polycomb group proteins to some of them. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

95F2-95F3

Sequence location

3R:24,223,445..24,226,934 [-]

Recombination map

3-84

RefSeq locus

NT_033777 REGION:24223445..24226934

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables sequence-specific DNA binding

inferred from direct assay

(Brown and Kassis, 2010, Brown et al., 2005)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN004619861

(GO Reference Genome Project, 2011-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN004619861

(GO Reference Genome Project, 2011-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in heterochromatin formation

inferred from mutant phenotype

(Brown and Kassis, 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN004619861

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(Brown and Kassis, 2010)

located_in polytene chromosome band

inferred from direct assay

(Brown and Kassis, 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN009066117

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SP1/KLF TRANSCRIPTION FACTORS

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SP1/KLF TRANSCRIPTION FACTORS -: The Sp1/KLF (Sp1-like/Kruppel-like factors) family of zinc finger proteins are DNA binding proteins. They can act as activators or repressors and some members bind sequences within Polycomb Response Elements (PREs). (Adapted from FBrf0188187).
C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Spps for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4KHC8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Stop-codon suppression (UAG) postulated; FBrf0216884.

Gene model reviewed during 5.44

Gene model reviewed during 5.39

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Spps-RA

FBtr0084565

NM_142975

3490

968

Spps-RB

FBtr0330382

NM_001275973

3490

985

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Spps-PA

FBpp0083950

102.0

968

6.01

FBpp0303408

104.1

985

5.88

A0A0B4KHC8

NP_001262902

AGB96282

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Spps using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Spps_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.53

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Brown and Kassis, 2010, Schaeper et al., 2010)

embryonic stage 1 -- 3

organism | intense | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

adult stage

adult mushroom body | restricted

(Kobayashi et al., 2006)

Additional Descriptive Data

Spps is maternally contributed and then expressed throughout development.

(Schaeper et al., 2010)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Brown and Kassis, 2010)

larval stage

organism | ubiquitous

(Brown and Kassis, 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from high throughput direct assay

(Sarov et al., 2016)

inferred from direct assay

(Brown and Kassis, 2010)

located_in polytene chromosome band

inferred from direct assay

(Brown and Kassis, 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Spps in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Spps

View exonic expression by tissue for Dmel\Spps

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039169

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42882/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039169

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039169

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039169

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5669

Flygut - An atlas of the Drosophila adult midgut

FBgn0039169

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Spps

Transgenic constructs containing regulatory region of Spps

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)crb87-4

(Brown and Kassis, 2010)

Df(3R)Exel6198

(Brown and Kassis, 2010, Ryder, 2004.4.26)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | germline clone | pharate adult stage

Spps¹

lethal | pharate adult stage | recessive

Spps¹

lethal - all die before end of P-stage (with Df(3R)crb87-4)

Spps¹

lethal - all die before end of P-stage (with Df(3R)Exel6198)

Spps¹

partially lethal - majority die | pharate adult stage | recessive

Spps¹

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Spps^GD5187

viable, with Scer\GAL4^pnr-MD237

Spps^GD5187

viable, with Scer\GAL4^tin.CΔ4

Spps^GD5187

Other Phenotypes

Allele

some die during pharate adult stage (with Df(3R)crb87-4)

Spps¹

some die during pharate adult stage (with Df(3R)Exel6198)

Spps¹

visible, with Scer\GAL4^Bx-MS1096

Spps^{UAS.ORF.GW.Tag:HA}

visible, with Scer\GAL4^pnr-MD237

Spps^GD5187

visible | cold sensitive, with Scer\GAL4^Bx-MS1096

Spps^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

mesonotum, with Scer\GAL4^pnr-MD237

Spps^GD5187

wing

Spps¹

wing, with Scer\GAL4^Bx-MS1096

Spps^{UAS.ORF.GW.Tag:HA}

wing | cold sensitive, with Scer\GAL4^Bx-MS1096

Spps^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (172)

Hsap\SP4

8 of 14

Yes

Hsap\SP1

7 of 14

Yes

Hsap\SP3

7 of 14

Yes

Hsap\SP2

4 of 14

Yes

Hsap\SP5

3 of 14

Yes

Hsap\SP6

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC344065

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZFP1

1 of 14

Yes

Hsap\ZFP37

1 of 14

Hsap\ZFP91

1 of 14

Yes

Hsap\ZFX

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF135

1 of 14

Hsap\ZNF140

1 of 14

Hsap\ZNF148

1 of 14

Yes

Hsap\ZNF154

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF358

1 of 14

Hsap\ZNF391

1 of 14

Yes

Hsap\ZNF397

1 of 14

Hsap\ZNF415

1 of 14

Hsap\ZNF416

1 of 14

Yes

Hsap\ZNF418

1 of 14

Hsap\ZNF420

1 of 14

Hsap\ZNF426

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ZNF701

1 of 14

Yes

Hsap\ZNF713

1 of 14

Yes

Hsap\ZNF717

1 of 14

Hsap\ZNF722P

1 of 14

Yes

Hsap\ZNF728

1 of 14

Hsap\ZNF732

1 of 14

Hsap\ZNF735

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (56)

Rnor\Sp4

8 of 14

Yes

Rnor\Sp1

7 of 14

Yes

Rnor\Sp3

6 of 14

Yes

Rnor\Sp2

4 of 14

Yes

Rnor\Sp5

4 of 14

Yes

Rnor\Sp6

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Yy2

1 of 14

Rnor\Zbtb7a

1 of 14

Yes

Rnor\Zfp17

1 of 14

Rnor\Zfp26

1 of 14

Rnor\Zfp69

1 of 14

Yes

Rnor\Zfp110

1 of 14

Yes

Rnor\Zfp112

1 of 14

Rnor\Zfp113

1 of 14

Yes

Rnor\Zfp180

1 of 14

Rnor\Zfp184

1 of 14

Rnor\Zfp300

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zxdc

1 of 14

Mus musculus (laboratory mouse) (93)

Mmus\Sp4

8 of 14

Yes

Mmus\Sp1

7 of 14

Yes

Mmus\Sp3

6 of 14

Yes

Mmus\Sp5

4 of 14

Yes

Mmus\Sp2

3 of 14

Yes

Mmus\Sp6

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Gm14418

1 of 14

Mmus\Gm14430

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp69

1 of 14

Yes

Mmus\Zfp110

1 of 14

Yes

Mmus\Zfp112

1 of 14

Mmus\Zfp113

1 of 14

Yes

Mmus\Zfp114

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zfp383

1 of 14

Yes

Mmus\Zfp420

1 of 14

Mmus\Zfp523

1 of 14

Yes

Mmus\Zfp560

1 of 14

Yes

Mmus\Zfp568

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (65)

Xtro\sp4

6 of 13

Yes

Xtro\sp3

5 of 13

Yes

Xtro\sp2

4 of 13

Yes

Xtro\sp5

4 of 13

Yes

Xtro\sp1

3 of 13

Yes

Xtro\klf7

2 of 13

Xtro\klf13

2 of 13

Yes

Xtro\klf15.2

2 of 13

Xtro\mapk7

2 of 13

Xtro\sp6

2 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100493797

1 of 13

Xtro\LOC100494833

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC101734910

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\wt1

1 of 13

Xtro\XB5772961

1 of 13

Yes

Xtro\XB5795368

1 of 13

Xtro\XB22063318

1 of 13

Yes

Xtro\zbtb38

1 of 13

Yes

Xtro\zbtb40

1 of 13

Yes

Xtro\zfp91

1 of 13

Yes

Xtro\znf91

1 of 13

Xtro\znf143

1 of 13

Yes

Xtro\znf281

1 of 13

Yes

Xtro\znf333

1 of 13

Xtro\znf367

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (68)

Drer\sp4

8 of 14

Yes

Drer\sp1

7 of 14

Yes

Drer\sp3b

7 of 14

Yes

Drer\sp3a

6 of 14

Yes

Drer\sp2

5 of 14

Yes

Drer\sp5b

4 of 14

Yes

Drer\sp5a

3 of 14

Yes

Drer\sp5l

3 of 14

Yes

Drer\sp6

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\klf12a

2 of 14

Drer\si:ch211-117k10.3

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:ch73-144d13.4

1 of 14

Yes

Drer\si:ch73-367f21.6

1 of 14

Drer\si:ch211-223a21.4

1 of 14

Yes

Drer\si:ch1073-127d16.1

1 of 14

Drer\si:dkey-33c14.6

1 of 14

Drer\wt1a

1 of 14

Drer\zbtb7c

1 of 14

Yes

Drer\zgc:153115

1 of 14

Drer\zgc:171422

1 of 14

Yes

Drer\zgc:171673

1 of 14

Drer\zgc:174275

1 of 14

Yes

Drer\zgc:174624

1 of 14

Drer\zic2a

1 of 14

Drer\znf76

1 of 14

Yes

Drer\znf143a

1 of 14

Yes

Drer\znf143b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\sptf-3

7 of 14

Yes

Cele\sptf-2

4 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP004438

9 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (4)

Atha\AT5G22890

1 of 13

Yes

Atha\DOT5

1 of 13

Yes

Atha\NTT

1 of 13

Yes

Atha\TT1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\FZF1

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Spps. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (25)

Sp1

4 of 13

btd

3 of 13

CG3065

3 of 13

CG42741

3 of 13

luna

3 of 13

cbt

2 of 13

dar1

2 of 13

hkb

2 of 13

Klf15

2 of 13

l(3)neo38

2 of 13

pad

2 of 13

Cf2

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

wor

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

SP6; Sp6 transcription factor

model of amelogenesis imperfecta type 1K

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SP4; Sp4 transcription factor

8 of 14

600540

SP1; Sp1 transcription factor

7 of 14

189906

SP3; Sp3 transcription factor

7 of 14

601804

SP2; Sp2 transcription factor

4 of 14

601801

SP5; Sp5 transcription factor

3 of 14

609391

SP6; Sp6 transcription factor

3 of 14

608613

AMELOGENESIS IMPERFECTA, TYPE IK; AI1K

amelogenesis imperfecta type 1K

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Spps

two hybrid

(Chetverina et al., 2022)

Spps - Blos1

two hybrid array

(Shokri et al., 2019)

Spps - CG10565

two hybrid array

(Shokri et al., 2019)

Spps - CG12219

two hybrid array

(Shokri et al., 2019)

Spps - CG8478

two hybrid array

(Shokri et al., 2019)

Spps - Ets65A

two hybrid array

(Shokri et al., 2019)

Spps - PolE4

two hybrid array

(Shokri et al., 2019)

Spps - Scm

two hybrid array

(Shokri et al., 2019)

Spps - caup

two hybrid array

(Shokri et al., 2019)

Spps - drm

two hybrid array

(Shokri et al., 2019)

Spps - eg

two hybrid array

(Shokri et al., 2019)

Spps - foxo

two hybrid array

(Shokri et al., 2019)

Spps - lbe

two hybrid array

(Shokri et al., 2019)

Spps - pho

two hybrid

(Chetverina et al., 2022)

Spps - retn

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pho

enhanceable

Spps

(Brown and Kassis, 2010)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

67813

DroID - A comprehensive database of gene and protein interactions.

FBgn0039169

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q2XXX8

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-84

Cytogenetic map

95F2-95F3

Sequence location

3R:24,223,445..24,226,934 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95F2-95F3

Limits computationally determined from genome sequence between P{PZ}Syx1A⁰⁶⁷³⁷&P{PZ}Syx1A¹⁰⁶⁶⁰ and P{PZ}crb⁰⁷²⁰⁷&P{PZ}BRWD3⁰⁵⁸⁴²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-84

(FlyBase, 2016)

3-82.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

67725

y¹ w^*; P{Spps-GFP.FPTB}attP40

30186

w¹¹¹⁸; P{EP}Spps^G8810/TM6C, Sb¹

81677

y¹ sc^* v¹ sev²¹; P{TOE.GS02740}attP40

62185

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05192}attP40

91589

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{neoFRT}82B Spps⁵²² P{PRE.Scr-PS1}Q1/TM3, Sb¹ Ser¹

FlyORF

F000477

M{UAS-Spps.ORF.3xHA.GW}ZH-86Fb

F004864

M{UAS-Spps.ORF-VNshort}ZH-86Fb

NIG-Fly

5669R-1

P{NIG.5669R}1

HMJ21777

y¹ v¹; P{TRiP.HMJ21777}attP40/CyO

VDRC

v30734

w¹¹¹⁸ P{GD5187}v30734

v45299

w¹¹¹⁸; P{GD5187}v45299

v318581

PBac{fTRG00426.sfGFP-TVPTBF}VK00033

More stocks available...

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (28)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD04007

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0039169

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0039169

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Brown and Kassis, 2010)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : 75 stable S2 cell lines containing a library of intrinsically disordered regions (IDRs) fluorescently tagged with mNeonGreen were constructed. In addition, stable S2 cell lines expressing the following full-length transcription factors were generated: da, rib, CG10321, CG13287, tgo, Spps, CG31510, brk, and disco.
(Hannon and Eisen, 2024)

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

The Spps gene may have been derived from the Sp1 gene by retroposition.

(Pan and Zhang, 2009)

Nomenclature History

Source for database identify of

Source for identity of: Spps CG5669

(Brown and Kassis, 2010)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG5669

(Gubala et al., 2017, Kumar et al., 2016, Sarov et al., 2016, Nadimpalli et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Jungreis et al., 2011, Brown and Kassis, 2010, Buffin and Gho, 2010, Ni et al., 2010.12.1, Schaeper et al., 2010, Pan and Zhang, 2009, Perkins et al., 2009, Munoz-Descalzo et al., 2007, Kobayashi et al., 2006, Brown et al., 2005, Suske et al., 2005)

Spps

(Brown et al., 2024, Hannon and Eisen, 2024, Brown et al., 2023, Erokhin et al., 2023, Chetverina et al., 2022, Cattenoz et al., 2021, Chetverina et al., 2020, Ghotbi et al., 2020, Kennison, 2020.10.22, Shokri et al., 2019, Bischof et al., 2018, Gubala et al., 2017, Transgenic RNAi Project members, 2017-, Ray et al., 2016, Schertel et al., 2015, dos Santos, 2014, McElroy et al., 2014, Langlais et al., 2012, Brown and Kassis, 2010)

Name Synonyms

Sp1-like factor for Pairing Sensitive-silencing

(Brown and Kassis, 2010)

Sp1-like factor for pairing sensitive-silencing

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42882

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4KHC8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4KHC8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0039169

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42882/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0039169

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0039169

FlyMine - An integrated database for Drosophila genomics

FBgn0039169

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5669

MARRVEL_MODEL - MARRVEL (model organism gene)

42882

Linkouts

BioGRID - A database of protein and genetic interactions.

67813

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0039169

DroID - A comprehensive database of gene and protein interactions.

FBgn0039169

DRSC - Results frm RNAi screens

FBgn0039169

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Spps_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0039169

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0039169

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5669

Flygut - An atlas of the Drosophila adult midgut

FBgn0039169

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q2XXX8

References (67)

The following external sites may use different assemblies or annotations than FlyBase.

-0.53

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