FB2026_03 , released September 17, 2026

CV Term Report

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General Information

Term

split fluorescent protein

ID (Ontology)

FBcv:0005052 (FlyBase CV)

Definition

Non-fluorescent fragment of a fluorescent protein. A functional fluorophore can be reconstituted if a split fluorescent protein fragment is expressed with a complementary split fluorescent protein fragment and the fragments are brought together in vivo, permitting the study of biological interactions. Protein-protein interactions can be studied using the bimolecular fluorescence complementation (BiFC) technique, where a fluorescent protein is reconstituted if the two complementary split fluorescent protein fragments are fused to interacting proteins or protein domains that bring the split halves together into the same macromolecular complex. Associations between closely apposed cells can be studied if the two complementary split fluorescent protein fragments are tethered to the surface of these cells, for example to study connectivity in the nervous system using the GFP Reconstitution Across Synaptic Partners (GRASP) technique.[ PubMed:11983170 PubMed:18255029 ]

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Records annotated with this term OR any of its CHILD TERMS

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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records

Full annotation statements	Relevant FlyBase reports
Full annotation statements	Experimental Tools
split fluorescent protein (all annotations which use CV term, excluding "NOT" statements)
encodes tool \| split fluorescent protein
tagged with \| split fluorescent protein

Spanning Tree (Parents/Children)

  split gene product detection tool
   |__split protein detection tool
       |__split fluorescent protein  29 rec.

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Parents/Children View Depth	Show hierarchy levels: for parents, for children
Relationships
Is a	split protein detection tool
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Synonyms & Secondary IDs
Synonyms

Secondary IDs

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