Gene model reviewed during 5.52
479 (aa); 55 (kD predicted)
Interacts with Sce.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ord using the Feature Mapper tool.
Protein is found in the nuclei of developing spermatocytes. It is extrachromosomal in stage S1-S3 spermatocytes (corresponding to early to mid G2 phase) but then becomes associated with chromatin during mid to late G2 phase. The ord protein is initially distributed uniformly over the chromatin but then is detected primarily at the centromere from metaphase of meiosis I through metaphase of meiosis II.
GBrowse - Visual display of RNA-Seq signalsView Dmel\ord in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Negative complementation occurs between strong alleles (typically showing poisoning activity) and weak alleles (typically are poisoned), it is not allele-specific, suggesting that protein-protein interactions are necessary for wild-type ord function. C-terminal part of ord is required to elicit negative complementation effects.
Meiotic sister-chromatid cohesion is severely disrupted in flies lacking ord protein and the frequency of missegregation in genetic tests suggests sister chromatid cohesion may be completely abolished. C-terminal part of ord is required for both aspects of ord function. ord function is not essential for cohesion during somatic mitosis as only a slight decrease in viability is observed.
Analysis of weak ord mutants suggests ord is required for proper centromeric cohesion after arm cohesion is released at the metaphase I/anaphase I transition. ord activity appears to promote centromeric cohesion during meiosis II but is not essential for kinetochore function during anaphase.
ord is necessary for chromosome segregation and can interact specifically with the centromere.
The C-terminal half of ord is essential for the normal function of the protein, unusual genetic interactions between specific ord alleles also implicates the C-terminal part in protein-protein interactions. In direction of increasing cytology: anon-59Da? ord-
ord is required to maintain sister-chromatid cohesion during meiosis in both males an females. Mutations result in defects in cohesion before the first division.
All alleles are fully viable, even in trans to a deficiency, therefore ord+ has no essential role in somatic mitosis, though a role in mitosis in the germ line is suggested.