FlyBase Allele Report: Dmel\ord[10]

General Information

Symbol

Dmel\ord¹⁰

Species

D. melanogaster

Name

FlyBase ID

FBal0059985

Feature type

allele

Associated gene

Dmel\ord

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Bickel and Orr-Weaver, 1997)

Mutagen

ethyl methanesulfonate

(Bickel et al., 1997)

Progenitor genotype

Cytology

Description

Nucleotide substitution: T?A.

(Bickel et al., 1997)

Amino acid replacement: L24term.

(Bickel et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,273,646..23,273,646 [-]

Nucleotide change:

T23273646A

Reported nucleotide change:

T?A

Amino acid change:

L24term | ord-PA; L24term | ord-PB

Reported amino acid change:

L24term

(Bickel et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Bickel et al., 1998, Bickel and Orr-Weaver, 1997)

abnormal meiotic cell cycle (with Df(2R)3-70)

(Bickel et al., 2002)

female semi-fertile

(Bickel et al., 1997)

viable

(Bickel et al., 1998)

Phenotype Manifest In

cell cycle (with Df(2R)3-70)

(Bickel et al., 2002)

chromosome (with ord⁵)

(Radford et al., 2015)

nuclear chromosome (with Df(2R)3-70)

(Bickel et al., 2002)

oocyte (with Df(2R)3-70)

(Bickel et al., 2002)

stage S1 oocyte | increased number

(Joyce and McKim, 2010)

Detailed Description

Statement

Reference

ord⁵/ord¹⁰ mutant oocytes display loss of co-orientation of homologous chromosomes as compared to wild type, but do not display loss of centromere orientation towards the spindle pole.

(Radford et al., 2015)

When ord⁴/ord¹⁰ female flies with normal X chromosomes are subjected to a 4-day aging regimen (after collection of virgin females and feeding with yeast paste overnight, the virgin females are kept for 4 days in the absence of males, which results in the majority of oocytes halting in developmental progression and aging within the female abdomen, as oviposition is suppressed), sex chromosome nondisjunction is not significantly greater in aged oocytes than in non-aged oocytes.

When ord⁸/ord¹⁰ female flies with normal X chromosomes are subjected to a 4-day aging regimen (after collection of virgin females and feeding with yeast paste overnight, the virgin females are kept for 4 days in the absence of males, which results in the majority of oocytes halting in developmental progression and aging within the female abdomen, as oviposition is suppressed), sex chromosome nondisjunction is significantly greater in aged oocytes than in non-aged oocytes. The majority of nondisjunction events are reductional in both aged and non-aged oocytes.

Meiotic crossovers on the X chromosomes are substantially reduced in ord⁴/ord¹⁰ and ord⁸/ord¹⁰ oocytes; the total map distance is reduced to 20% or less of the wild type value.

When subjected to the aging regimen, FM7a/X ; ord⁴/ord¹⁰ aged oocytes show a significant increase in nondisjunction that lasted for 48 hours. Reductional nondisjunction events predominate in both aged and non-aged oocytes.

When subjected to the aging regimen, Df(1)bb158/X ; ord⁴/ord¹⁰ females show age-dependent nondisjunction; sex chromosome nondisjunction is significantly greater in aged oocytes than in non-aged oocytes.

When subjected to the aging regimen, In(1)dl-49/X ; ord⁴/ord¹⁰ females do not show age-dependent nondisjunction.

Heterochromatin pairing between the FM7a chromosome and a normal X chromosome is significantly disrupted in ord⁴/ord¹⁰ oocytes.

(Subramanian and Bickel, 2009)

DNA double-strand breaks occur at approximately the same frequency in ord¹⁰ nuclei and are formed and repaired as in wild-type. Recovery of the ring X chromosome R(1)2 relative to a normal rod X chromosome during female meiosis is significantly lowered in ord⁵/ord¹⁰ (ratio of ring/rod=0.2 compared to 0.7 in wild-type) and ord⁶/ord¹⁰ (ratio of ring/rod=0.4 compared to 0.7 in wild-type) mutants.

(Webber et al., 2004)

Oocytes from ord¹⁰/Df(2R)3-70 mothers exhibit sister-chromatid cohesion defects. The cohesion affects are not seen until after nuclear envelope breakdown. Loss of cohesion is seen in 45% of prometaphase I figures. In addition oocytes are unable to arrest at metaphase I. and cohesion defects are obvious in 72% of oocytes that progress beyond metaphase I. Unlike in wild-type oocytes from ord¹⁰/Df(2R)3-70 fail to arrest at Metaphase I, though oocytes are sometimes seen in prometaphase I. Other effects in segregation are also seen. The level of meiotic recombination in ord¹⁰/Df(2R)3-70 oocytes is about 16% of wild-type.

(Bickel et al., 2002)

Random segregation of chromosomes due to complete loss of sister chromatid cohesion. Females do not produce any oocytes arrested at metaphase I following residual exchange events.

(Bickel and Orr-Weaver, 1997)

Hemizygotes are viable and exhibit no cuticular, bristle or eye phenotypes but have reduced female fertility. Light microscope analysis of chromosomes reveal no gross abnormalities reflecting aberrant segregation during the germ-line mitotic divisions. Orcein stained testes squashes reveals loosely organised bivalents with single chromatids protruding from the chromosome mass. Staining reveals initial homologue association is not compromised severely in males. Unlike the wild-type situation, precocious separation of sister chromatid is readily apparent in males during meiosis II. Cohesion is totally absent before anaphase II and aberrant anaphase II figures with unequal polar distribution of chromatids are often seen, consistent with the production of aneuploid gametes. No metaphase II figures are observed. In the absence of ord protein (ord¹⁰ hemizygotes or ord¹⁰/ord⁵ transheterozygotes) sister chromatid cohesion is missing and individual chromatids segregate randomly to the poles with minimal chromosome loss.

(Bickel et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

FM7a, ord⁸/ord¹⁰ has abnormal meiotic cell cycle phenotype

(Jeffreys et al., 2003)

FM7a, ord¹⁰/ord⁴ has abnormal meiotic cell cycle phenotype

(Jeffreys et al., 2003)

mei-S332¹, ord¹⁰ has viable phenotype

(Bickel et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

ord¹⁰ has stage S1 oocyte | increased number phenotype, suppressible by pch2^EY01788a

(Joyce and McKim, 2010)

Enhancer of

Statement

Reference

ord[+]/ord¹⁰ is an enhancer of oocyte | oogenesis stage S12 phenotype of Incenp^QA26

(Resnick et al., 2009)

ord[+]/ord¹⁰ is an enhancer of oocyte | oogenesis stage S13 phenotype of Incenp^QA26

(Resnick et al., 2009)

Other

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰, vtd^GL00522 has ovary phenotype

(Gyuricza et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰, vtd^GL00522 has female germline cell phenotype

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has oocyte phenotype

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has chromosome | female | adult stage phenotype

(Gyuricza et al., 2016)

SA1^GL00534, Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰ has ovary phenotype

(Gyuricza et al., 2016)

SA1^GL00534, Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰ has female germline cell phenotype

(Gyuricza et al., 2016)

Incenp^QA26, ord[+]/ord¹⁰ has synaptonemal complex phenotype

(Resnick et al., 2009)

Incenp^QA26, ord[+]/ord¹⁰ has oocyte | oogenesis stage S14 phenotype

(Resnick et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of SA^GL00534 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the ord⁵/ord¹⁰ mutant background results in rudimentary ovaries lacking germ cells.

c(2)M^EP2115/c(2)M^Z0810,ord⁵/ord¹⁰ double mutants show defects in centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes relative to controls.

(Gyuricza et al., 2016)

Assembly of the synaptonemal complex is normal in the ovaries of Incenp^QA26 ord¹⁰/Incenp^QA26 females. However, these females show premature disassembly of the synaptonemal complex.

85% of stage 12 and 13 oocytes show prometaphase I configurations with separated chromosomes in Incenp^QA26 ord¹⁰/Incenp^QA26 females, and at stage 14, 53% of the oocytes have aberrant metaphase I chromosome configurations. The X and fourth chromosomes show a failure of bivalent biorientation.

(Resnick et al., 2009)

ord⁴/ord¹⁰ or ord⁸/ord¹⁰ animals also carrying FM7a balancer an age dependant increase in meiotic non-disjunction is seen.

(Jeffreys et al., 2003)

Double mutants of mei-S332¹ with ord¹⁰ are fully viable. Eyes, bristles and cuticles appear normal. Fertility is no worse than that seen for ord¹⁰ single mutants. Double mutants show the meiotic non-disjunction phenotype characteristic of ord¹⁰ mutants.

(Bickel et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ord¹⁰/Df(2R)3-70 is rescued by ord^mNeonGreen

(Lehner, 2022.10.20)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

607063

y¹ w^*/Dp(1;Y)y⁺; ord¹⁰ bw¹; P{gfp::ord.B}T076

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ord¹⁰

(Lehner, 2022.10.20, Hatkevich et al., 2019, Das et al., 2016, Gyuricza et al., 2016, Radford et al., 2015, Joyce and McKim, 2010, Resnick et al., 2009, Subramanian and Bickel, 2009, Page et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (16)

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