Expression of brp^dsRNA.B.UAS and brp^dsRNA.C.UAS under the control of Scer\GAL4^GMR.PF significantly reduces retinal depolarization level and ON transients, compared to controls; there is no effect on the transition of R7 photoreceptor axon terminals from filopodial to bouton-like structures during pupal development in MARCM clones of this genotype, compared to controls.

Knockdown of brp by expression of brp^{dsRNA.C.Scer\UAS} alone or in combination with brp^{dsRNA.B.Scer\UAS} under the control of Scer\GAL4^ey-OK107 does not cause a conspicuous morphological change of the mushroom body.

Knockdown of brp in Kenyon cells through expression of brp^{dsRNA.C.Scer\UAS} alone or in combination with brp^{dsRNA.B.Scer\UAS} under the control of Scer\GAL4^ey-OK107 significantly impairs memory measured at three hours after training. Anesthesia-resistant memory is affected, while anesthesia-sensitive memory is not affected. brp^{dsRNA.B.Scer\UAS} expression under the control of Scer\GAL4^ey-OK107, Scer\GAL4^Mef2.247 or Scer\GAL4^c739 does not lead to a significant reduction of avoidance of electric shock or odors compared with the corresponding controls.

The ERGs of flies that express brp^{dsRNA.C.Scer\UAS} under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^GMR.PF show light-induced depolarization of the photoreceptor cells but the ON/OFF transients are missing. The eye morphology of Scer\GAL4^GMR.PF>brp^{dsRNA.C.Scer\UAS} flies is normal. The photoreceptor terminals these flies lack clear T-bars but contain conspicuous presynaptic densities.

Larvae expressing brp^{dsRNA.C.Scer\UAS}, under the control of Scer\GAL4^elav-C155, show no defects in NMJ size, numbers of presynaptic boutons, postsynaptic receptor field size or postsynaptic density. The amplitudes and frequency of spontaneous miniature junctional currents (mEJCs) are not significantly different from those of wild type. However, amplitudes of evoked junctional currents (EJCs) are reduced by about 50% compared to wild type and quantal content is decreased. The overall structure of boutons appears normal but the number of T-bars is severely reduced.

Flies expressing brp^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^elav-C155 show locomotor defects. These flies fail to show negative geotaxis as they do not climb vertically in an empty food vial within 30 seconds. Spontaneous walking is also severely reduced compared to wild-type flies as judged by the number of lines of a 2x2cm grid crossed within 30 seconds. Additionally, these flies cannot sustain flight and quickly crash to the ground.

Scer\GAL4^elav-C155/brp^RNAi.C.UAS is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of unc-104^bris/unc-104^d11204

Scer\GAL4^elav-C155/brp^RNAi.C.UAS is a non-enhancer of bouton | third instar larval stage phenotype of unc-104^bris/unc-104^d11204