FlyBase Allele Report: Dmel\brp[RNAi.B.UAS]

General Information

Symbol

Dmel\brp^RNAi.B.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0195570

Feature type

allele

Associated gene

Dmel\brp

Associated Insertion(s)

Carried in Construct

P{UAS-brp.RNAi.B}

Also Known As

UAS-brp-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Wagh et al., 2006)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Wagh et al., 2006)

Mutagen

in vitro construct

(Wagh et al., 2006)

Progenitor genotype

Carried in construct

P{UAS-brp.RNAi.B}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a brp fragment that corresponds to an exonic region.

(Wagh et al., 2006)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

brp

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

retina, with Scer\GAL4^GMR.PF, brp^RNAi.C.UAS

(Özel et al., 2019)

Detailed Description

Statement

Reference

Expression of brp^dsRNA.B.UAS and brp^dsRNA.C.UAS under the control of Scer\GAL4^GMR.PF significantly reduces retinal depolarization level and ON transients, compared to controls; there is no effect on the transition of R7 photoreceptor axon terminals from filopodial to bouton-like structures during pupal development in MARCM clones of this genotype, compared to controls.

(Özel et al., 2019)

Knockdown of brp by expression of brp^{dsRNA.B.Scer\UAS} alone or in combination with brp^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^ey-OK107 does not cause a conspicuous morphological change of the mushroom body.

Knockdown of brp in Kenyon cells through expression of brp^{dsRNA.B.Scer\UAS} alone or in combination with brp^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^ey-OK107 significantly impairs memory measured at three hours after training. Anesthesia-resistant memory is affected, while anesthesia-sensitive memory is not affected. brp^{dsRNA.B.Scer\UAS} expression under the control of Scer\GAL4^ey-OK107, Scer\GAL4^Mef2.247 or Scer\GAL4^c739 does not lead to a significant reduction of avoidance of electric shock or odors compared with the corresponding controls.

Knockdown of brp in Kenyon cells through expression of brp^{dsRNA.B.Scer\UAS} in combination with brp^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^ey-OK107 significantly impairs aversive olfactory short-term memory testing at 5 minutes after training.

Knockdown of brp in Kenyon cells through expression of brp^{dsRNA.B.Scer\UAS} in combination with brp^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^ey-OK107, Scer\GAL4^Mef2.247 or Scer\GAL4^c739 causes a significant defect in anesthesia-resistant memory. Addition of Scer\GAL80^Mef2.PT significantly improves anesthesia-resistant memory in Scer\GAL4^ey-OK107 and Scer\GAL4^c739-driven knockdowns.

(Knapek et al., 2011)

Depending on the insertion, flies that express brp^{dsRNA.B.Scer\UAS} under the control of Scer\GAL4^GMR.PF have a rough eye phenotype or normal eye morphology. One brp^{dsRNA.B.Scer\UAS} insertion expressed under the control of Scer\GAL4^elav-C155 causes embryonic lethality while another insertion is viable.

The locomotor activity of flies expressing brp^{dsRNA.B.Scer\UAS} under the control of Scer\GAL4^elav-C155 is reduced compared to wild-type flies.

(Wagh et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/brp^RNAi.B.UAS is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of unc-104^bris/unc-104^d11204

(Kern et al., 2013)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/brp^RNAi.B.UAS is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of unc-104^bris/unc-104^d11204

(Kern et al., 2013)

Scer\GAL4^elav-C155/brp^RNAi.B.UAS is a non-enhancer of bouton | third instar larval stage phenotype of unc-104^bris/unc-104^d11204

(Kern et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Knockdown of brp by expression of brp^{dsRNA.B.Scer\UAS} alone or in combination with brp^{dsRNA.C.Scer\UAS} under the control of Scer\GAL4^ey-OK107 in a rut²⁰⁸⁰ mutant background results in a significantly larger memory defect tahn in either knockdown/mutant alone. Unlike controls, these flies show no detectable memory at 3 hours. The avoidance of shock and odors is not significantly impaired in these flies.

(Knapek et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Transfected into flies to study the phenotypic consequences of dsRNA interference (RNAi) of the brp gene.

(Wagh et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

brp^RNAi.B.UAS

brp^{dsRNA.B.Scer\UAS}

brp^dsRNA.B.UAS

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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