FlyBase Allele Report: Dmel\Ipk2[20B]

General Information

Symbol

Dmel\Ipk2^20B

Species

D. melanogaster

Name

FlyBase ID

FBal0319431

Feature type

allele

Associated gene

Dmel\Ipk2

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Seeds et al., 2015)

Mutagen

Delta2-3 transposase

(Seeds et al., 2015)

Progenitor genotype

P{EP}Ipk2^G3545

(Seeds et al., 2015)

Cytology

Description

Deletion of the entire Ipk2 open reading frame. Generated by imprecise excision of P{EP}Ipk2^G3545.

(Seeds et al., 2015)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Pupation in Ipk2^20B/Ipk2^20B (zygotic) mutants is delayed by around 12 hours compared to wild type. Ipk2^20B/Ipk2^20B pupae have rough eyes with missing or disordered ommatidia and bristles that are progressively less defective from anterior to posterior, smaller wings than wild type, disordered or missing bristles on the notum and 56% of mutants form symmetrical degenerative holes on their posterior notums (with a similar percentage developing a single hole in the thoracic region of their ventral midline). Ipk2^20B/Ipk2^20B mutants show increased cell death and cellular proliferation defects (reduced DNA synthesis) in third instar larval wing discs (but not other tissues).

Ipk2^20B/Ipk2^20B germline clones (to identify any maternal contributions) arrest as pupae; these pupae fail to form an adult epidermis during pupation (including head, thorax, wing, abdomen and legs), and at the third instar larval stage they lack imaginal discs.

(Seeds et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Ipk2^20B has increased cell death | third instar larval stage phenotype, suppressible by Dronc^I29/Dronc^I29

(Seeds et al., 2015)

Ipk2^20B has visible | P-stage phenotype, suppressible | partially by hop^Tum/hop[+]

(Seeds et al., 2015)

Ipk2^20B has hypoplasia | P-stage phenotype, suppressible | partially by hop^Tum/hop[+]

(Seeds et al., 2015)

Ipk2^20B has lethal - all die before end of P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has visible | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has abnormal size | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has lethal - all die before end of P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has visible | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has abnormal size | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has increased cell death | third instar larval stage phenotype, suppressible by Scer\GAL4^en.PU/BacA\p35^UAS.cUa

(Seeds et al., 2015)

NOT suppressed by

Statement

Reference

Ipk2^20B has increased cell death | third instar larval stage phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Seeds et al., 2015)

Ipk2^20B has increased cell death | third instar larval stage phenotype, non-suppressible by Scer\GAL4^en.PU/bsk^DN.UAS.cUa

(Seeds et al., 2015)

Ipk2^20B has lethal - all die before end of P-stage phenotype, non-suppressible by hop^Tum/hop[+]

(Seeds et al., 2015)

Suppressor of

Statement

Reference

Ipk2^20B/Ipk2^20B is a suppressor of visible | pharate adult stage phenotype of Scer\GAL4^GMR.PU, upd1^UAS.cZa

(Seeds et al., 2015)

BacA\p35^UAS.cUa, Scer\GAL4^GMR.PU, Ipk2^20B/Ipk2^20B is a suppressor of visible | pharate adult stage phenotype of Scer\GAL4^GMR.PU, upd1^UAS.cZa

(Seeds et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ipk2^20B has notopleural bristle | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has presutural bristle | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has wing | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has ommatidium | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has interommatidial bristle | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has mesothoracic postnotum | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has notopleural bristle | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has presutural bristle | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has wing | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Atha\IPK2a^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has imaginal disc | third instar larval stage phenotype, suppressible by Scer\GAL4^en.PU/BacA\p35^UAS.cUa

(Seeds et al., 2015)

Ipk2^20B has wing disc | third instar larval stage phenotype, suppressible by Dronc^I29/Dronc^I29

(Seeds et al., 2015)

Ipk2^20B has wing | P-stage phenotype, suppressible | partially by hop^Tum/hop[+]

(Seeds et al., 2015)

Ipk2^20B has ommatidium | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has interommatidial bristle | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

Ipk2^20B has mesothoracic postnotum | P-stage phenotype, suppressible by Scer\GAL4^Act.PU/Scer\ARG82^UAS.GFP

(Seeds et al., 2015)

NOT suppressed by

Statement

Reference

Ipk2^20B has wing disc | third instar larval stage phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Seeds et al., 2015)

Ipk2^20B has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4^en.PU/bsk^DN.UAS.cUa

(Seeds et al., 2015)

Suppressor of

Statement

Reference

Ipk2^20B/Ipk2^20B is a suppressor of eye | pharate adult stage phenotype of Scer\GAL4^GMR.PU, upd1^UAS.cZa

(Seeds et al., 2015)

BacA\p35^UAS.cUa, Scer\GAL4^GMR.PU, Ipk2^20B/Ipk2^20B is a suppressor of eye | pharate adult stage phenotype of Scer\GAL4^GMR.PU, upd1^UAS.cZa

(Seeds et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Dronc^I29/Dronc^I29 suppresses increased cell death seen in Ipk2^20B/Ipk2^20B third instar larval wing discs.

p53^5A-1-4/p53^5A-1-4 or expression of bsk^{DN.Scer\UAS.cUa} driven by Scer\GAL4^en.PU does not suppress increased cell death seen in Ipk2^20B/Ipk2^20B third instar larval wing discs.

Ipk2^20B/Ipk2^20B suppresses the enlarged eyes seen in pharate adults with expression of upd1^Scer\UAS.cZa driven by Scer\GAL4^GMR.PU.

hop^Tum/+ partially suppresses the small wing phenotype but does not suppress lethality in Ipk2^20B/Ipk2^20B pupae.

(Seeds et al., 2015)

Xenogenetic Interactions

Statement

Reference

Expression of Scer\ARG82^{Scer\UAS.T:Avic\GFP} or Atha\IPK2a^{Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^Act.PU rescues lethality and developmental morphological defects in Ipk2^20B/Ipk2^20B mutants.

Expression of BacA\p35^Scer\UAS.cUa driven by Scer\GAL4^en.PU in wing discs suppresses increased cell death seen in Ipk2^20B/Ipk2^20B mutants.

Ipk2^20B/Ipk2^20B suppresses the enlarged eyes seen in pharate adults with expression of upd1^Scer\UAS.cZa driven by Scer\GAL4^GMR.PU, even with co-expression of BacA\p35^Scer\UAS.cUa.

(Seeds et al., 2015)

Complementation and Rescue Data

Rescued by

Ipk2^20B is rescued by Scer\GAL4^Act.PU/Ipk2^UAS.GFP

(Seeds et al., 2015)

Not rescued by

Ipk2^20B is not rescued by Scer\GAL4^Act.PU/Ipk2^{D276A.UAS.GFP}

(Seeds et al., 2015)

Comments

Expression of Ipk2^{Scer\UAS.T:Avic\GFP} (but not Ipk2^{D276A.Scer\UAS.T:Avic\GFP} driven by Scer\GAL4^Act.PU rescues lethality and morphological defects in Ipk2^20B/Ipk2^20B mutants.

(Seeds et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ipk2^20B

ipk2^20B

(Seeds et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (1)

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