FlyBase Allele Report: Dmel\S6k[UAS.cMa]

General Information

Symbol

Dmel\S6k^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Montagne

FlyBase ID

FBal0103997

Feature type

allele

Associated gene

Dmel\S6k

Associated Insertion(s)

Carried in Construct

P{UAS-S6k.M}

Also Known As

UAS-dS6K, UAS-S6k

Key Links

Transgenic product class

wild_type

(Montagne et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Montagne et al., 1999)

Mutagen

in vitro construct

(Montagne et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-S6k.M}

Cytology

Description

UAS sequences drive S6k expression.

(Montagne et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

S6k

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Lv et al., 2012)

ommatidium, with Scer\GAL4^GMR.PF

(Lv et al., 2012)

salivary gland, with Scer\GAL4^AB1

(Georgiev et al., 2010)

wing, with Scer\GAL4^ap-md52

(Montagne et al., 1999)

wing, with Scer\GAL4^ap-md544

(Gluderer et al., 2008, Barcelo and Stewart, 2002, Radimerski et al., 2002, Rintelen et al., 2001)

Detailed Description

Statement

Reference

Expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 results in an increase in larval body weight compared to controls.

(Gupta et al., 2013)

Overexpression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF, does not induce autophagy in developing eye discs.

(Shin et al., 2013)

Overexpression of S6k^Scer\UAS.cMa in the developing eye, under the control of Scer\GAL4^GMR.PF, causes large, disorganised eyes.

(Lv et al., 2012)

Expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^AB1 results in a 32% increase average cell size in the salivary gland.

(Georgiev et al., 2010)

Ectopic expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 within the developing dorsal wing compartment induces a moderate overgrowth in this unit and a bending-down of the adult wing.

(Montagne et al., 2010)

Expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 results in a bent down wing phenotype.

(Gluderer et al., 2008)

Expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 causes the wing to bend down.

(Barcelo and Stewart, 2002)

Expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 causes a bending down of the wing. Animals expressing S6k^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF have a normal sized eye.

(Radimerski et al., 2002)

Expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 results in a bent-wing phenotype.

(Rintelen et al., 2001)

S6k^Scer\UAS.cMa driven by Scer\GAL4^ap-md52 produces wings that are convex and bent downwards.

(Montagne et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by bun^GE12327, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by bun^A.UAS, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, enhanceable by Pdk1^EP837, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

Suppressed by

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^GMR.PF has increased cell growth rate phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, suppressible by bun^B.UAS, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, suppressible | partially by bun^C.UAS, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, suppressible | partially by Akt^UAS.cRa, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, suppressible by Pdk1^4ΔEP/Pdk1^5ΔEP, Scer\GAL4^ap-md544

(Rintelen et al., 2001)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has visible phenotype, suppressible by Pk61C[+]/Pdk1^5ΔEP, Scer\GAL4^ap-md544

(Rintelen et al., 2001)

NOT Enhancer of

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^Bx-MS1096 is a non-enhancer of visible phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

S6k^UAS.cMa, Scer\GAL4^GMR.PF is a non-enhancer of visible phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Suppressor of

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, Tak1^UAS.cTa

(Shin et al., 2013)

S6k^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PF

(Kim et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ppk.PG/S6k^UAS.cMa is a non-suppressor of abnormal neuroanatomy | larval stage phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

S6k^UAS.cMa, Scer\GAL4^Bx-MS1096 is a non-suppressor of visible phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by bun^GE12327, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by bun^A.UAS, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, enhanceable by Pdk1^EP837, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

Suppressed by

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

S6k^UAS.cMa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF

(Lv et al., 2012)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, suppressible by bun^B.UAS, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, suppressible | partially by bun^C.UAS, Scer\GAL4^ap-md544

(Gluderer et al., 2008)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, suppressible | partially by Akt^UAS.cRa, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, suppressible by Pdk1^4ΔEP/Pdk1^5ΔEP, Scer\GAL4^ap-md544

(Rintelen et al., 2001)

S6k^UAS.cMa, Scer\GAL4^ap-md544 has wing phenotype, suppressible by Pk61C[+]/Pdk1^5ΔEP, Scer\GAL4^ap-md544

(Rintelen et al., 2001)

NOT Enhancer of

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^Bx-MS1096 is a non-enhancer of wing phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

S6k^UAS.cMa, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^GMR.PF

(Radimerski et al., 2002)

Suppressor of

Statement

Reference

S6k^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Dcp-1^UAS.cKa, Scer\GAL4^GMR.PF

(Kim et al., 2010)

S6k^UAS.cMa, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa

(Montagne et al., 2010)

S6k^UAS.cMa, Scer\GAL4^ey.PH is a suppressor of eye phenotype of Pten^UAS.cHa, Scer\GAL4^ey.PH

(Montagne et al., 2010)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ppk.PG/S6k^UAS.cMa is a non-suppressor of larval multidendritic class IV neuron phenotype of path^dg50/Df(3L)BSC773

(Lin et al., 2015)

S6k^UAS.cMa, Scer\GAL4^Bx-MS1096 is a non-suppressor of wing phenotype of Pi3K92E^UAS.Tag:MYC, Scer\GAL4^Bx-MS1096

(Radimerski et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The number of autolysosomes, indicating the level of autophagy, is decreased upon co-expression of S6k^Scer\UAS.cMa and Tak1^Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4^GMR.PF, compared to expression of Tak1^Scer\UAS.cTa alone.

(Shin et al., 2013)

Depletion of Hdac3 in the eye, through expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PF, strongly suppresses the overgrown, disorganised eye phenotype (with increased ommatidial size) found upon expression of S6k^Scer\UAS.cMa in the eye. These double-mutant flies exhibit a phenotype similar to Hdac3^{dsRNA.Sym.Scer\UAS} single mutants.

(Lv et al., 2012)

Expression of S6k^Scer\UAS.cMa suppresses the rough eye phenotype seen when Dcp-1^Scer\UAS.cKa (insertion line P{UAS-Dcp-1.K}19-2) is expressed under the control of Scer\GAL4^GMR.PF.

(Kim et al., 2010)

Co-expression of S6k^UAS.cMa fails to suppress the reduction in growth of the developing eye seen upon expression of Pten^UAS.cHa under the control of Scer\GAL4^ey.PH.

Co-expression of S6k^UAS.cMa suppresses the reduction in growth of the developing eye seen upon expression of Tsc1^UAS.cTa and gig^UAS.cTa under the control of Scer\GAL4^ey.PH.

Co-induction of Hr3^{dsRNA.UAS.cMa} suppresses the bent-down wing phenotype seen upon induction of both S6k^UAS.cMa and Hr3^RB.UAS in the developing wing disc under the control of Scer\GAL4^ap-md544.

(Montagne et al., 2010)

Co-expression of one of bun^GE12327 or bun^A.Scer\UAS enhances the bent down wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544.

Co-expression of bun^B.Scer\UAS completely suppresses the bent down wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544.

Co-expression of bun^C.Scer\UAS substantially suppresses the bent down wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544.

(Gluderer et al., 2008)

Co-expression of Akt1^Scer\UAS.cRa slightly suppresses the bent wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544. Co-expression of Pk61C^EP837 enhances the bent wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544. Co-expression of S6k^Scer\UAS.cMa does not result in any further increase in eye size in animals expressing Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF. Co-expression of S6k^Scer\UAS.cMa does not modify the bent wing phenotype caused by expression of Pi3K92E^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096.

(Radimerski et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6910

w¹¹¹⁸; P{UAS-S6k.M}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

S6k^Scer\UAS.cMa

S6k^UAS.cMa

p70s6K^Scer\UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Montagne

(Montagne et al., 1999)

Secondary FlyBase IDs

References (21)

Report Sections

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