FlyBase Allele Report: Dmel\Pdk1[EP837]

General Information

Symbol

Dmel\Pdk1^EP837

Species

D. melanogaster

Name

FlyBase ID

FBal0124094

Feature type

allele

Associated gene

Dmel\Pdk1

Associated Insertion(s)

P{EP}EP837

Carried in Construct

Also Known As

EP837

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Radimerski et al., 2002, Cho et al., 2001, Gene Disruption Project members, 2001-, Rintelen et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{EP}EP837

Cytology

Description

The P{EP} insertion is 179bp upstream of the Pk61C transcription start site.

(Cho et al., 2001)

P{EP} insertion 3875bp upstream of the putative start codon of Pk61C. P{EP} element is inserted so that expression from the Scer\UAS sequences in the element drives overexpression of Pk61C.

(Rintelen et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:131,762..131,762 [+]

(Eddison et al., 2011, Jones et al., 2009, Chan et al., 2007, Reiling et al., 2005, Reiling and Hafen, 2004, Radimerski et al., 2002, Cho et al., 2001, Gene Disruption Project members, 2001-, Rintelen et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Eddison et al., 2011)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Coronavirus infectious disease

modeled by HCoV-SARS\M^{UAS.CUHK-Su10}

(Chan et al., 2007)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant

(Jones et al., 2009)

chemical sensitive, with Scer\GAL4^elav-C155

(Eddison et al., 2011)

increased cell size, with Scer\GAL4^GMR.PF

(Cho et al., 2001)

visible, with Scer\GAL4^ap-md544

(Cho et al., 2001)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Cho et al., 2001, Rintelen et al., 2001)

ommatidium, with Scer\GAL4^GMR.PF

(Cho et al., 2001)

wing, with Scer\GAL4^ap-md544

(Cho et al., 2001)

Detailed Description

Statement

Reference

Neuronal expression of Pdk1^EP837 under the control of Scer\GAL4^elav-C155 increases sensitivity to ethanol sedation.

(Eddison et al., 2011)

Age-related locomotor impairment is significantly delayed in mutant males compared to controls.

Mutant males show increased resistance to paraquat compared to controls.

(Jones et al., 2009)

Expression of Pk61C^EP837 under the control of Scer\GAL4^ap-md544 has no effect on the wing.

(Radimerski et al., 2002)

Expression of Pk61C^EP837 when driven by Scer\GAL4^GMR.PF causes an increase in ommatidial size (about 1.33 fold bigger than wild-type). When driven by Scer\GAL4^ap-md544 the wing is convex towards the dorsal side.

(Cho et al., 2001)

Expression of Pk61C^EP837 under the control of Scer\GAL4^GMR.PF results in a slight but significant increase in eye size. Expression of Pk61C^EP837 under the control of Scer\GAL4^GMR.PB in clones of cells in the eye does not increase cell size.

(Rintelen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype, suppressible by Lk6^EP30.18

(Reiling et al., 2005)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype, suppressible by chrb^EP1035

(Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype, suppressible by scyl^EP22.1

(Reiling and Hafen, 2004)

Enhancer of

Statement

Reference

Pdk1^EP837, Scer\GAL4^ap-md544 is an enhancer of visible phenotype of S6k^UAS.cMa, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

Other

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased body size phenotype

(Reiling et al., 2005, Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has increased cell size | somatic clone | cell autonomous phenotype

(Rintelen et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

Pdk1^EP837, Scer\GAL4^GMR.PF has interommatidial bristle phenotype, enhanceable by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pdk1^EP837, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by Akt^UAS.cRa, Scer\GAL4^GMR.PF

(Rintelen et al., 2001)

NOT Enhanced by

Statement

Reference

Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, non-enhanceable by Pka-R1^BDK.UAS, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Suppressed by

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Lk6^EP30.18

(Reiling et al., 2005)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Lk6^EP30.18, Scer\GAL4^GMR.PF

(Reiling et al., 2005)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by chrb^EP1035

(Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, suppressible by scyl^EP22.1

(Reiling and Hafen, 2004)

NOT suppressed by

Statement

Reference

Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype, non-suppressible by Pka-R1^BDK.UAS, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Enhancer of

Statement

Reference

Pdk1^EP837, Scer\GAL4^ap-md544 is an enhancer of wing phenotype of S6k^UAS.cMa, Scer\GAL4^ap-md544

(Radimerski et al., 2002)

Pdk1^EP837, Scer\GAL4^GMR.PF is an enhancer of interommatidial bristle phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pdk1^EP837, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Pdk1^EP837, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Akt^UAS.Tag:HA, Scer\GAL4^GMR.PF

(Cho et al., 2001)

NOT Enhancer of

Statement

Reference

Pdk1^EP837, Scer\GAL4^GMR.PF is a non-enhancer of eye phenotype of Pka-C1^UAS.cKa, Scer\GAL4^GMR.PF

(Cho et al., 2001)

NOT Suppressor of

Statement

Reference

Pdk1^EP837, Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Pka-C1^UAS.cKa, Scer\GAL4^GMR.PF

(Cho et al., 2001)

Other

Statement

Reference

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye phenotype

(Reiling et al., 2005, Reiling and Hafen, 2004)

Akt^UAS.cRa, Pdk1^EP837, Scer\GAL4^GMR.PF has eye | somatic clone | cell autonomous phenotype

(Rintelen et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Animals expressing both Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large-eye phenotype. Expression of Lk6^EP30.18 in this background strongly suppresses this phenotype.

(Reiling et al., 2005)

Flies co-expressing Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF have a large eye phenotype. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of scyl^EP22.1. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed by co-expression of chrb^EP1035. The large eye phenotype caused by co-expression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF is suppressed nearly to wild type by simultaneous co-expression of both scyl^EP22.1 and chrb^EP1035.

(Reiling and Hafen, 2004)

Co-expression of Pk61C^EP837 enhances the bent wing phenotype caused by expression of S6k^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544.

(Radimerski et al., 2002)

When Pk61C^EP837 and Akt1^{Scer\UAS.T:Ivir\HA1} are coexpressed under the control of Scer\GAL4^GMR.PF, the eyes display a severely crumpled morphology. The eye bristles are enlarged even more severely, and the boundaries of all ommatidia and photoreceptor cells disappear. The addition of Pka-R1^BDK.Scer\UAS to Pk61C^EP837, Akt1^{Scer\UAS.T:Ivir\HA1} flies has no effect on the eye phenotype.

(Cho et al., 2001)

Coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PF causes a substantial increase in eye size. Coexpression of Akt1^Scer\UAS.cRa and Pk61C^EP837 under the control of Scer\GAL4^GMR.PB in clones of cells in the eye results in a cell autonomous increase in cell size.

(Rintelen et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

PDK-1^EP837

(Chan et al., 2007)

PDK1^EP837

(Jones et al., 2009)

Pdk1^EP837

Pk61C^EP0837

Pk61C^EP837

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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