FlyBase Allele Report: Dmel\HDAC3[RNAi.Sym.UAS]

General Information

Symbol

Dmel\HDAC3^RNAi.Sym.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0288279

Feature type

allele

Associated gene

Dmel\HDAC3

Associated Insertion(s)

Carried in Construct

P{UAS-HDAC3.RNAi.Sym}

Key Links

Transgenic product class

RNAi_reagent

(Lv et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Lv et al., 2012)

Mutagen

in vitro construct

(Lv et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-HDAC3.RNAi.Sym}

Cytology

Description

UAS regulatory sequences drive expression of a HDAC3 cDNA fragment (between 691 and 1198bp in its open reading frame) that can be transcribed bi-directionally to produce dsRNA.

(Lv et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

HDAC3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased body size, with Scer\GAL4^ppl.PP

(Lv et al., 2012)

decreased cell growth rate, with Scer\GAL4^e22c

(Lv et al., 2012)

decreased cell growth rate, with Scer\GAL4^ey.PH

(Lv et al., 2012)

lethal - all die during larval stage, with Scer\GAL4^Act5C.PI

(Lv et al., 2012)

partially lethal, with Scer\GAL4^ppl.PP

(Lv et al., 2012)

Phenotype Manifest In

embryonic/larval fat body, with Scer\GAL4^ppl.PP

(Lv et al., 2012)

eye, with Scer\GAL4^ey.PH

(Lv et al., 2012)

ommatidium, with Scer\GAL4^ey.PH

(Lv et al., 2012)

wing, with Scer\GAL4^e22c

(Lv et al., 2012)

Detailed Description

Statement

Reference

Expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ey.PH results in a reduction in the size of the eye. The average area of male fly eyes is reduced by approximately 30%, compared to controls. The depletion of Hdac3 results in an 11% reduction in ommatidial size. The total number of ommatidia is also reduced, with an approximately 24% reduction.

Expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^e22c results in a reduction in the posterior compartment of the wing.

Expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ppl.PP in the fat body results in progeny that are partially lethal, with the surviving progeny exhibiting a small body size at the 3rd-instar larval, pupal and adult stages. The average weight of the Hdac3 RNAi female progeny is ~16% less than that of controls, while the weight of male progeny is reduced by ~14%.

(Lv et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

HDAC3^RNAi.Sym.UAS, Scer\GAL4^ey.PH has decreased cell growth rate phenotype, suppressible | partially by His4^K16A.UAS, Scer\GAL4^ey.PH

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^ppl.PP has decreased body size phenotype, suppressible | partially by His4^K16A.UAS, Scer\GAL4^ppl.PP

(Lv et al., 2012)

Suppressor of

Statement

Reference

HDAC3^RNAi.Sym.UAS, Scer\GAL4^e22c is a suppressor of increased cell growth rate phenotype of Pi3K92E^UAS.Exel, Scer\GAL4^e22c

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of increased cell growth rate phenotype of S6k^UAS.cMa, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of increased cell growth rate phenotype of Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF

(Lv et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

HDAC3^RNAi.Sym.UAS, Scer\GAL4^ey.PH has eye phenotype, suppressible | partially by His4^K16A.UAS, Scer\GAL4^ey.PH

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^ey.PH has ommatidium phenotype, suppressible | partially by His4^K16A.UAS, Scer\GAL4^ey.PH

(Lv et al., 2012)

Suppressor of

Statement

Reference

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of S6k^UAS.cMa, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of S6k^UAS.cMa, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of InR^UAS.Exel, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of InR^UAS.Exel, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Pi3K92E^UAS.Exel, Scer\GAL4^GMR.PF

(Lv et al., 2012)

HDAC3^RNAi.Sym.UAS, Scer\GAL4^e22c is a suppressor of wing phenotype of Pi3K92E^UAS.Exel, Scer\GAL4^e22c

(Lv et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Depletion of Hdac3 in the eye, through expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PF, strongly suppresses the overgrown, disorganised eye phenotype (with increased ommatidial size) found upon expression of InR^{Scer\UAS.Exel} in the eye. These double-mutant flies exhibit a phenotype similar to Hdac3^{dsRNA.Sym.Scer\UAS} single mutants.

Overexpression of His4^{K16A.Scer\UAS} in a Hdac3^{dsRNA.Sym.Scer\UAS} background partially attenuates the Hdac3^{dsRNA.Sym.Scer\UAS}-induced decrease in eye size. Consistent with this finding, the reduction in body weight induced by Hdac3^{dsRNA.Sym.Scer\UAS} is also partially attenuated by co-expression of His4^{K16A.Scer\UAS}.

Depletion of Hdac3 in the eye, through expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PF, strongly suppresses the overgrown, disorganised eye phenotype (with increased ommatidial size) found upon expression of Pi3K92E^{Scer\UAS.Exel} in the eye. These double-mutant flies exhibit a phenotype similar to Hdac3^{dsRNA.Sym.Scer\UAS} single mutants.

Depletion of Hdac3 in the eye, through expression of Hdac3^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^GMR.PF, strongly suppresses the overgrown, disorganised eye phenotype (with increased ommatidial size) found upon expression of S6k^Scer\UAS.cMa in the eye. These double-mutant flies exhibit a phenotype similar to Hdac3^{dsRNA.Sym.Scer\UAS} single mutants.

(Lv et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

HDAC3^RNAi.Sym.UAS

HDAC3^{dsRNA.Sym.Scer\UAS}

HDAC3^{dsRNA.Sym.UAS}

Hdac3^{dsRNA.Sym.Scer\UAS}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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