FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\LamChs.PS
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General Information
Symbol
Dmel\LamChs.PS
Species
D. melanogaster
Name
heat shock construct of Stuurman
FlyBase ID
FBal0097229
Feature type
allele
Associated gene
Dmel\LamC
Associated Insertion(s)
Carried in Construct
P{hs-LamC.S}
Key Links
Transgenic product class
frameshift_variant
(Stuurman et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
frameshift_variant
(Stuurman et al., 1999)
Mutagen
in vitro construct
(Stuurman et al., 1999)
Progenitor genotype
Carried in construct
P{hs-LamC.S}
Cytology
Description

An Hsp70 promoter drives expression of a LamC cDNA, with a single base deletion (nucleotide 1781), resulting in a frameshift which replaces the sequence after amino acid residue 552 until the C-terminal end (residue 621) with 59 unrelated amino acids.

(Stuurman et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
LamC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      larval salivary gland & embryo & nucleus | conditional ts

      (Stuurman et al., 1999)

      melanotic mass | heat sensitive

      (Stuurman et al., 1999)
      Detailed Description
      Statement
      Reference

      Embryos expressing LamChs.PS for 30 minutes at 3-5 hours of development using heat shock develop into normal, fertile flies. Expression of LamChs.PS using heat shock between 48 and 120 hours of development (second larval instar to early pupal stage) results in lethality. When LamChs.PS is expressed using heat shock during the third larval instar stage, most of the larvae do not develop into pupae, but remain alive as larvae for up to 8 days longer than control larvae. The growth of these larvae is inhibited compared to control larvae. Many of these larvae have melanotic tumours. The nuclei of salivary gland cells appear smaller than in control larvae.

      (Stuurman et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      LamChs.PS
      Name Synonyms
      heat shock construct of Stuurman
      (Stuurman et al., 1999)
      Secondary FlyBase IDs
        References (2)