FlyBase Allele Report: Dmel\LamC[EX265]

General Information

Symbol

Dmel\LamC^EX265

Species

D. melanogaster

Name

FlyBase ID

FBal0192073

Feature type

allele

Associated gene

Dmel\LamC

Associated Insertion(s)

P{?PTT-GB}LamC^EX265

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Schulze et al., 2005)

Progenitor genotype

P{PTT-GB}LamC^G00158

(Schulze et al., 2005)

Associated Insertion(s)

P{?PTT-GB}LamC^EX265

Cytology

Description

Imprecise excision of the P{PTT-GB} element, which leaves the LamC coding region intact. A 1.4kb portion of the original insertion appears to still be present (this portion lacks Avic\GFP and functional w[+] sequences). This lesion is located within both the LamC and ttv transcription units (since LamC is nested within an intron of ttv). However, only LamC function is affected, since the "EX265" chromosome complements ttv^00681b and fails to complement LamC^EP2199.

(Schulze et al., 2005)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with Df(2R)trix)

(Schulze et al., 2005)

lethal (with LamC^EP2199)

(Schulze et al., 2005)

lethal (with LamC^EX3)

(Schulze et al., 2005)

lethal (with LamC^EX5)

(Schulze et al., 2005)

lethal (with LamC^EX16)

(Schulze et al., 2005)

lethal (with LamC^EX187)

(Schulze et al., 2005)

lethal (with LamC^EX296)

(Li et al., 2016, Schulze et al., 2005)

lethal (with LamC^EX364)

(Schulze et al., 2005)

lethal (with LamC^G00158)

(Schulze et al., 2005)

lethal (with LamC^UM-8373-3)

(Schulze et al., 2005)

lethal | recessive

(Schulze et al., 2005)

Phenotype Manifest In

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with LamC^EX296)

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with LamC^EX296), with LamC^E174K.UAS, Scer\GAL4^BG487

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage (with LamC^EX296), with LamC^R564C.UAS, Scer\GAL4^BG487

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 4 | third instar larval stage (with LamC^EX296)

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 4 | third instar larval stage (with LamC^EX296), with LamC^E174K.UAS, Scer\GAL4^BG487

(Li et al., 2016)

muscle cell of A1-7 ventral longitudinal muscle 4 | third instar larval stage (with LamC^EX296), with LamC^R564C.UAS, Scer\GAL4^BG487

(Li et al., 2016)

NMJ bouton | larval stage (with LamC^EX296)

(Li et al., 2016)

nuclear lamina | third instar larval stage (with LamC^EX296), with LamC^E174K.UAS, Scer\GAL4^BG487

(Li et al., 2016)

nuclear lamina | third instar larval stage (with LamC^EX296), with LamC^R564C.UAS, Scer\GAL4^BG487

(Li et al., 2016)

Detailed Description

Statement

Reference

LamC^EX265/LamC^EX296 transheterozygotes are lethal before adulthood and their third instar larval muscles 6 and 7 exhibit a significantly increased proportion of 'ghost' neuromuscular junctions, but not of abnormal nuclear lamina blebs, as compared to controls.

The expression of either LamC^{E174K.Scer\UAS} or LamC^{R564C.Scer\UAS}, but not of LamC^Scer\UAS.cLa, under the control of Scer\GAL4^BG487 in LamC^EX265/LamC^EX296 transheterozygotes leads to a strong increase in the number of nuclei exhibiting more than 10 abnormal lamina blebs in third instar larval muscles 6 and 7, as compared to controls.

(Li et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

LamC^EX364

(Schulze et al., 2005)

LamC^EX296

(Schulze et al., 2005)

LamC^EX187

(Schulze et al., 2005)

LamC^EX3

(Schulze et al., 2005)

LamC^EX5

(Schulze et al., 2005)

LamC^EX16

(Schulze et al., 2005)

LamC^UM-8373-3

(Schulze et al., 2005)

LamC^G00158

(Schulze et al., 2005)

LamC^EP2199

(Schulze et al., 2005)

Rescued by

LamC^EX296/LamC^EX265 is rescued by Scer\GAL4^Mhc.PW/LamC^UAS.cLa

(Li et al., 2016)

Not rescued by

LamC^EX296/LamC^EX265 is not rescued by Scer\GAL4^Mhc.PW/LamC^E174K.UAS

(Li et al., 2016)

LamC^EX296/LamC^EX265 is not rescued by Scer\GAL4^Mhc.PW/LamC^R564C.UAS

(Li et al., 2016)

Comments

LamC^EX265/LamC^EX296 transheterozygotes exhibit lethality before adulthood, which is not rescued by the expression of LamC^{R564C.Scer\UAS} under the control of Scer\GAL4^Mhc.PW; LamC^EX265/LamC^EX296 transheterozygotes also exhibit an increased proportion of 'ghost' neuromuscular junctions in third instar larval muscles 6 and 7, which is rescued by the expression of LamC^Scer\UAS.cLa, but not of LamC^{E174K.Scer\UAS} or LamC^{R564C.Scer\UAS}, under the control of Scer\GAL4^Mhc.PW.

(Li et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

LamC^EX265

lamC^EX265

(Li et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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