FlyBase Allele Report: Dmel\AMPKα[T184D.UAS]

General Information

Symbol

Dmel\AMPKα^T184D.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0241312

Feature type

allele

Associated gene

Dmel\AMPKα

Associated Insertion(s)

Carried in Construct

P{UAS-AMPKα.T184D}

Also Known As

ampk^T184D, UAS-AMPKα^T184D

Key Links

Transgenic product class

missense_variant

(Swick et al., 2013)

polypeptide_post_translational_processing_variant

(Swick et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Swick et al., 2013)

polypeptide_post_translational_processing_variant

(Swick et al., 2013)

Mutagen

in vitro construct

(Swick et al., 2013)

Progenitor genotype

Carried in construct

P{UAS-AMPKα.T184D}

Cytology

Description

UASt regulatory sequences drive expression of a phosphomimetic-activated form of AMPKα (carries the amino acid replacement T184D).

(Swick et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

AMPKα

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates obesity

modeled by Hsap\SNCAIP^UAS.cLa

(Liu et al., 2021)

ameliorates congenital disorder of deglycosylation 1

modeled by Pngl^ex14

(Han et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Wong et al., 2015)

increased body weight | adult stage, with Scer\GAL4^elav.PU

(Liu et al., 2021)

increased rate of feeding behavior | adult stage, with Scer\GAL4^elav.PU

(Liu et al., 2021)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

(Wong et al., 2015)

NMJ bouton | third instar larval stage, with Scer\GAL4^elav-C155

(Wong et al., 2015)

Detailed Description

Statement

Reference

Ectopic expression of AMPKα^{T184D.Scer\UAS} under the control of the Scer\GAL4^elav-C155 driver results in a decreased number of neuromuscular junction boutons in third instar larvae compared to wild-type controls.

(Wong et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

AMPKα^T184D.UAS, Scer\GAL4^elav.PU is a non-enhancer of increased rate of feeding behavior | adult stage phenotype of Hsap\SNCAIP^UAS.cLa, Scer\GAL4^elav.PU

(Liu et al., 2021)

AMPKα^T184D.UAS, Scer\GAL4^elav.PU is a non-enhancer of increased body weight | adult stage phenotype of Hsap\SNCAIP^UAS.cLa, Scer\GAL4^elav.PU

(Liu et al., 2021)

Scer\GAL4^elav-C155/AMPKα^T184D.UAS is a non-enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

NOT Suppressor of

Statement

Reference

AMPKα^T184D.UAS, Scer\GAL4^elav.PU is a non-suppressor of increased rate of feeding behavior | adult stage phenotype of Hsap\SNCAIP^UAS.cLa, Scer\GAL4^elav.PU

(Liu et al., 2021)

AMPKα^T184D.UAS, Scer\GAL4^elav.PU is a non-suppressor of increased body weight | adult stage phenotype of Hsap\SNCAIP^UAS.cLa, Scer\GAL4^elav.PU

(Liu et al., 2021)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155/AMPKα^T184D.UAS is a non-enhancer of neuromuscular junction | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

Scer\GAL4^elav-C155/AMPKα^T184D.UAS is a non-enhancer of NMJ bouton | third instar larval stage phenotype of Trpml¹

(Wong et al., 2015)

NOT Suppressor of

Statement

Reference

AMPKα^T184D.UAS, Scer\GAL4^fln.IFM is a non-suppressor of indirect flight muscle cell | adult stage phenotype of LamC^R205W.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

AMPKα^T184D.UAS, Scer\GAL4^fln.IFM is a non-suppressor of indirect flight muscle cell | adult stage phenotype of LamC^G489V.UAS, Scer\GAL4^fln.IFM

(Chandran et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of LamC^UAS.cSa and AMPKα^T184D.UAS under the control of Scer\GAL4^fln.IFM does not lead to any obvious indirect flight muscle, wing posture or flight defects.

(Chandran et al., 2019)

Ectopic expression of AMPKα^{T184D.Scer\UAS} under the control of the Scer\GAL4^elav-C155 driver does not cause further decrease in the number of neuromuscular junction (NMJ) boutons in the already NMJ boutons-deficient Trpml¹ mutant third instar larvae.

(Wong et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32110

w¹¹¹⁸; P{UAS-AMPKα.T184D}2/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

AMPK^T184D

(Suzuta et al., 2022)

AMPKα^{T184D.Scer\UAS}

AMPKα^T184D.UAS

SNF1A^{T184D.Scer\UAS}

ampk^T184D

(Wong et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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