FlyBase Allele Report: Dmel\Keap1[KK102175]

General Information

Symbol

Dmel\Keap1^KK102175

Species

D. melanogaster

Name

FlyBase ID

FBal0231649

Feature type

allele

Associated gene

Dmel\Keap1

Associated Insertion(s)

Carried in Construct

P{KK102175}

Also Known As

Keap1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK102175}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK102175

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal gravitaxis | adult stage, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

abnormal sleep | adult stage, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

long lived, with Scer\GAL4^elav-C155

(Spiers et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Keap1^KK102175 under the control of Scer\GAL4^elav-C155 leads to significant changes in electrophysiology of third instar larval neuromuscular junctions (NMJs): potently increased spontaneous EJC (mEJC) amplitudes (without any changes in mEJC frequencies or evoked EJC (eEJC) amplitudes), decreased quantal content (QC), reduced number of releasable quanta and increased paired-pulse ratios compared to controls. It also leads to an enhanced performance in negative geotaxis-mediated activities in adults while it does not lead to any changes in third instar larval crawling distances compared to controls.

Expression of Keap1^KK102175 under the control of Scer\GAL4^elav-C155 does not lead to any changes in survival of adults under continual heat stress-challenged conditions when compared to controls.

Expression of Keap1^KK102175 under the control of Scer\GAL4^elav-C155 does not lead to any changes in EJC amplitudes (spontaneous or evoked), QC or number of releasable quanta of third instar larval NMJs 24 hours after a 1 hour of heat shock challenge compared to controls.

Expression of Keap1^KK102175 under the control of Scer\GAL4^elav-C155 leads to a significant increase in sleep bout length during both day and night time while it leads to a significant decrease in sleep bout number during day but not night time when compared to control adults. And it leads to a significant decrease in the night but not day time activity of adults as compared to controls.

(Spiers et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Keap1^KK102175, Scer\GAL4^HCH.Hand is an enhancer of short lived phenotype of LamC^G489V.UAS, Scer\GAL4^HCH.Hand

(Bhide et al., 2018)

Other