FlyBase Allele Report: Dmel\Mrp4[EP3177]

General Information

Symbol

Dmel\Mrp4^EP3177

Species

D. melanogaster

Name

FlyBase ID

FBal0261006

Feature type

allele

Associated gene

Dmel\Mrp4

Associated Insertion(s)

P{EP}Mrp4^EP3177

Carried in Construct

Also Known As

EP3177

Genomic Maps

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Allele class

hypomorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Huang and Haddad, 2007)

Mutagen

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable element insertion site

3R:11,569,162..11,569,162 [+]

(Huang et al., 2014, Huang and Haddad, 2007)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

P{EP}Mrp4^EP3177 is inserted 88 bp upstream of the Mrp4 transcription start site and directs over-expression of Mrp4 in the presence of Scer\GAL4.

(Huang and Haddad, 2007)

Insertion components

P{EP}Mrp4^EP3177

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

misexpression element

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Scer\GAL4^yolk

(Huang et al., 2014)

abnormal oxidative stress response | adult stage | RU486 conditional, with Scer\GAL4^Switch1.106

(Huang et al., 2014)

abnormal oxidative stress response | adult stage | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Huang et al., 2014)

abnormal stress response, with Scer\GAL4^Act.PU

(Huang and Haddad, 2007)

abnormal stress response, with Scer\GAL4^da.G32

(Huang and Haddad, 2007)

abnormal stress response, with Scer\GAL4^Ddc.PL

(Huang and Haddad, 2007)

abnormal stress response, with Scer\GAL4^drl.442

(Huang and Haddad, 2007)

abnormal stress response, with Scer\GAL4^elav.PU

(Huang and Haddad, 2007)

abnormal stress response (with Mrp4^EP3221)

(Huang and Haddad, 2007)

abnormal stress response (with Mrp4^EP3328)

(Huang and Haddad, 2007)

abnormal stress response (with Mrp4^ex621)

(Huang and Haddad, 2007)

abnormal stress response (with Mrp4^ex661)

(Huang and Haddad, 2007)

abnormal stress response | recessive

(Huang and Haddad, 2007)

lethal - all die before end of larval stage | conditional, with Scer\GAL4^da.G32

(Huang and Haddad, 2007)

lethal - all die before end of larval stage | conditional, with Scer\GAL4^elav.PU

(Huang and Haddad, 2007)

long lived | RU486 conditional, with Scer\GAL4^{αTub84B.Switch.PK}

(Huang et al., 2014)

short lived, with Scer\GAL4^da.PU

(Huang et al., 2014)

some die during larval stage | conditional, with Scer\GAL4^da.G32

(Huang and Haddad, 2007)

some die during larval stage | conditional, with Scer\GAL4^elav.PU

(Huang and Haddad, 2007)

Phenotype Manifest In

Detailed Description

Statement

Reference

In adult flies fed RU486, Mrp4^EP3177 overexpressed by Scer\GAL4^{αTub84B.Switch.PK} or Scer\GAL4^Switch1.106 significantly improves survival rates in response to oxidative stress compared to wild type. Overexpression of Mrp4^EP3177 by Scer\GAL4^yolk increases survival rates in response to oxidative stress (paraquat) compared to wild type.

With RU486 feeding, Mrp4^EP3177 overexpressed by Scer\GAL4^{αTub84B.Switch.PK} (but not Scer\GAL4^Switch1.106) extends lifespan compared to siblings not fed RU486. Mrp4^EP3177 overexpression by Scer\GAL4^da.PU shortens lifespan compared to wild type.

(Huang et al., 2014)

Flies expressing Mrp4^EP3177 using Scer\GAL4^da.G32, Scer\GAL4^Act.PU, Scer\GAL4^elav.PU, Scer\GAL4⁴⁴² or Scer\GAL4^Ddc.PL, but not Scer\GAL4^repo.PU or Scer\GAL4^how-24B, display a profound increase in recovery time after anoxia compared with controls.

Mrp4^EP3177/Mrp4^EP3177, Mrp4^EP3177/Mrp4^EP3221 and Mrp4^EP3177/Mrp4^EP3328 flies show an increase in recovery time after anoxia compared with controls.

Mrp4^ex11/Mrp4^EP3177 and Mrp4^ex31/Mrp4^EP3177 flies show the same recovery time after anoxia as controls.

Mrp4^EP3177/Mrp4^ex661 and Mrp4^EP3177/Mrp4^ex621 flies show an increase in recovery time after anoxia compared with controls.

No pupae form when Mrp4^EP3177 is expressed with Scer\GAL4^da.G32 or Scer\GAL4^elav.PU under chronic hypoxia conditions. This is in contrast to Scer\GAL4^how-24B Mrp4^EP3177 flies and controls which show no larval developmental defects and die during late pupal stages under the same conditions.

(Huang and Haddad, 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Mrp4^EP3177

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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